Abstract
The optimal light intensity required for photosynthesis by mesophyll protoplasts of pea (Pisum sativum) is about 1250 microeinsteins per square meter per second. On exposure to supra-optimal light intensity (2500 microeinsteins per square meter per second) for 10 min, the protoplasts lost 30 to 40% of their photosynthetic capacity. Illumination with normal light intensity (1250 microeinsteins per square meter per second) for 10 min enhanced the rate of dark respiration in protoplasts. On the other hand, when protoplasts were exposed to photoinhibitory light, their dark respiration also was markedly reduced along with photosynthesis. The extent of photoinhibition was increased when protoplasts were incubated with even low concentrations of classic respiratory inhibitors: 1 micromolar antimycin A, 1 micromolar sodium azide, and 1 microgram per milliliter oligomycin. At these concentrations, the test inhibitors had very little or no effect directly on the process of photosynthetic oxygen evolution. The promotion of photoinhibition by inhibitors of oxidative electron transport (antimycin A, sodium azide) and phosphorylation (oligomycin) was much more pronounced than that by inhibitors of glycolysis and tricarboxylic acid cycle (sodium fluoride and sodium malonate, respectively). We suggest that the oxidative electron transport and phosphorylation in mitochondria play an important role in protecting the protoplasts against photoinhibition of photosynthesis. Our results also demonstrate that protoplasts offer an additional experimental system for studies on photoinhibition.
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Claims 9
Claim · model-written summary
Optimal light intensity for pea protoplast photosynthesis was about 1250 microeinsteins per square meter per second.
- Taxon
- pea (Pisum sativum)
Lathyrus oleraceuse0 - Organ / stage
- mesophyll protoplastse0
- Outcome
- optimal light intensity required for photosynthesise0
- Value
- 1250 microeinsteins per square meter per seconde0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The optimal light intensity required for photosynthesis by mesophyll protoplasts of pea (Pisum sativum) is about 1250 microeinsteins per square meter per second”
Claim · model-written summary
Ten minutes of supra-optimal light reduced photosynthetic capacity by 30 to 40%.
- Taxon
- Pea (Pisum sativum)Lathyrus oleraceust0
- Organ / stage
- protoplastse0
- Treatment
- supra-optimal light intensity (2500 microeinsteins per square meter per second) for 10 mine0
- Outcome
- photosynthetic capacitye0
- Direction
- loste0
- Value
- 30 to 40%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On exposure to supra-optimal light intensity (2500 microeinsteins per square meter per second) for 10 min, the protoplasts lost 30 to 40% of their photosynthetic capacity”
t0“Pea (Pisum sativum)”
Claim · model-written summary
Normal light for 10 minutes enhanced dark respiration.
- Taxon
- Pea (Pisum sativum)Lathyrus oleraceust0
- Organ / stage
- protoplastse0
- Treatment
- normal light intensity (1250 microeinsteins per square meter per second) for 10 mine0
- Outcome
- rate of dark respiratione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Illumination with normal light intensity (1250 microeinsteins per square meter per second) for 10 min enhanced the rate of dark respiration in protoplasts”
t0“Pea (Pisum sativum)”
Claim · model-written summary
Photoinhibitory light markedly reduced dark respiration and photosynthesis.
- Taxon
- Pea (Pisum sativum)Lathyrus oleraceust0
- Organ / stage
- protoplastse0
- Treatment
- photoinhibitory lighte0
- Outcome
- dark respiration also was markedly reduced along with photosynthesise0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“when protoplasts were exposed to photoinhibitory light, their dark respiration also was markedly reduced along with photosynthesis”
t0“Pea (Pisum sativum)”
Claim · model-written summary
Low concentrations of respiratory inhibitors increased photoinhibition.
- Taxon
- Pea (Pisum sativum)Lathyrus oleraceust0
- Organ / stage
- protoplastse0
- Treatment
- classic respiratory inhibitorse0
- Outcome
- extent of photoinhibitione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extent of photoinhibition was increased when protoplasts were incubated with even low concentrations of classic respiratory inhibitors: 1 micromolar antimycin A, 1 micromolar sodium azide, and 1 microgram per milliliter oligomycin”
t0“Pea (Pisum sativum)”
Claim · model-written summary
At these concentrations, the inhibitors had little or no direct effect on photosynthetic oxygen evolution.
- Taxon
- Pea (Pisum sativum)Lathyrus oleraceust0
- Treatment
- test inhibitorse0
- Outcome
- photosynthetic oxygen evolutione0
- Direction
- very little or no effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At these concentrations, the test inhibitors had very little or no effect directly on the process of photosynthetic oxygen evolution”
t0“Pea (Pisum sativum)”
Claim · model-written summary
Oxidative electron transport and phosphorylation inhibitors promoted photoinhibition more than glycolysis and tricarboxylic acid cycle inhibitors.
- Taxon
- Pea (Pisum sativum)Lathyrus oleraceust0
- Treatment
- inhibitors of oxidative electron transport (antimycin A, sodium azide) and phosphorylation (oligomycin)e0
- Outcome
- promotion of photoinhibitione0
- Direction
- much more pronouncede0
- Comparator
- inhibitors of glycolysis and tricarboxylic acid cycle (sodium fluoride and sodium malonate, respectively)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The promotion of photoinhibition by inhibitors of oxidative electron transport (antimycin A, sodium azide) and phosphorylation (oligomycin) was much more pronounced than that by inhibitors of glycolysis and tricarboxylic acid cycle (sodium fluoride and sodium malonate, respectively)”
t0“Pea (Pisum sativum)”
Claim · model-written summary
The authors infer mitochondrial oxidative electron transport and phosphorylation protect protoplasts from photoinhibition.
- Taxon
- Pea (Pisum sativum)Lathyrus oleraceust0
- Outcome
- protecting the protoplasts against photoinhibition of photosynthesise0
- Qualifier
- oxidative electron transport and phosphorylation in mitochondriae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“oxidative electron transport and phosphorylation in mitochondria play an important role in protecting the protoplasts against photoinhibition of photosynthesis”
t0“Pea (Pisum sativum)”
Claim · model-written summary
Pea protoplasts were proposed as an additional experimental system for photoinhibition studies.
- Taxon
- Pea (Pisum sativum)Lathyrus oleraceust0
- Outcome
- experimental system for studies on photoinhibitione0
- Comparator
- additionale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protoplasts offer an additional experimental system for studies on photoinhibition”
t0“Pea (Pisum sativum)”
The same questions in other papers
oligomycin · physiology: photosynthesis · Lathyrus oleraceus (pea)Results agree
1 paper in the same direction as this paper
- negative
light · physiology: respiration · Lathyrus oleraceus (pea)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- no effect
light · physiology: photosynthesis · Lathyrus oleraceus (pea)Positive and negative results
13 papers in the same direction as this paper · 10 in the opposite direction
- positive
- positive
- positive
20 papers more in this group
See all 24 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.