Abstract

Barley kernels germinated in water, in solutions of gibberellic acid (GA), in chloramphenicol sodium succinate (CAL), and in combinations of GA and CAL, for 5 days were analyzed daily to determine growth and α- and β-amylase, proteinase, and respiratory activities. Respiration, growth, and amylase activities were inhibited by CAL, but were accelerated by GA. Combined treatment of CAL and GA partially reversed the inhibitive effects of CAL on respiration and growth, but amylase development approached that of GA-treated seeds. No correlations were found between proteinase activity and respiration or α-or β-amylase activities. The significance of these findings with respect to α-amylase formation and mode of action of GA is discussed. It is suggested that α-amylase is synthesized during germination and that GA accelerates this synthesis.

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Claims 8

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

CAL inhibited barley respiration and growth.

Treatment
CALe0t0
Outcome
Respiration, growthe0t0
Direction
inhibitede0t0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Respiration, growth, and amylase activities were inhibited by CAL, but were accelerated by GA.”

✓ located verbatim in the source · abstract, characters 266–360 · show in text ↑
t0

“MECHANISM OF ACTION OF GIBBERELLIC ACID: INHIBITION OF α-AMYLASE DEVELOPMENT DURING GERMINATION OF BARLEY BY CHLORAMPHENICOL AND ITS REVERSAL BY GIBBERELLIC ACID”

✓ located verbatim in the source · title, characters 0–161 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

CAL inhibited barley amylase activity.

Treatment
CALe0t0
Outcome
amylase activitiese0t0
Direction
inhibitede0t0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Respiration, growth, and amylase activities were inhibited by CAL, but were accelerated by GA.”

✓ located verbatim in the source · abstract, characters 266–360 · show in text ↑
t0

“MECHANISM OF ACTION OF GIBBERELLIC ACID: INHIBITION OF α-AMYLASE DEVELOPMENT DURING GERMINATION OF BARLEY BY CHLORAMPHENICOL AND ITS REVERSAL BY GIBBERELLIC ACID”

✓ located verbatim in the source · title, characters 0–161 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GA accelerated barley respiration and growth.

Treatment
GAe0t0
Outcome
Respiration, growthe0t0
Direction
acceleratede0t0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Respiration, growth, and amylase activities were inhibited by CAL, but were accelerated by GA.”

✓ located verbatim in the source · abstract, characters 266–360 · show in text ↑
t0

“MECHANISM OF ACTION OF GIBBERELLIC ACID: INHIBITION OF α-AMYLASE DEVELOPMENT DURING GERMINATION OF BARLEY BY CHLORAMPHENICOL AND ITS REVERSAL BY GIBBERELLIC ACID”

✓ located verbatim in the source · title, characters 0–161 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GA accelerated barley amylase activity.

Treatment
GAe0t0
Outcome
amylase activitiese0t0
Direction
acceleratede0t0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Respiration, growth, and amylase activities were inhibited by CAL, but were accelerated by GA.”

✓ located verbatim in the source · abstract, characters 266–360 · show in text ↑
t0

“MECHANISM OF ACTION OF GIBBERELLIC ACID: INHIBITION OF α-AMYLASE DEVELOPMENT DURING GERMINATION OF BARLEY BY CHLORAMPHENICOL AND ITS REVERSAL BY GIBBERELLIC ACID”

✓ located verbatim in the source · title, characters 0–161 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

GA partially reversed CAL inhibition of respiration and growth.

Treatment
Combined treatment of CAL and GAe0t0
Outcome
respiration and growthe0t0
Direction
partially reversede0t0
Comparator
CALe0t0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“Combined treatment of CAL and GA partially reversed the inhibitive effects of CAL on respiration and growth”

✓ located verbatim in the source · abstract, characters 361–468 · show in text ↑
t0

“MECHANISM OF ACTION OF GIBBERELLIC ACID: INHIBITION OF α-AMYLASE DEVELOPMENT DURING GERMINATION OF BARLEY BY CHLORAMPHENICOL AND ITS REVERSAL BY GIBBERELLIC ACID”

✓ located verbatim in the source · title, characters 0–161 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Amylase development with combined CAL and GA approached that of GA-treated seeds.

Treatment
CAL and GAe0t0e3
Outcome
amylase developmente0t0e3
Direction
approachede0t0e3
Comparator
GA-treated seedse0t0e3

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“amylase development approached that of GA-treated seeds.”

✓ located verbatim in the source · abstract, characters 474–530 · show in text ↑
t0

“MECHANISM OF ACTION OF GIBBERELLIC ACID: INHIBITION OF α-AMYLASE DEVELOPMENT DURING GERMINATION OF BARLEY BY CHLORAMPHENICOL AND ITS REVERSAL BY GIBBERELLIC ACID”

✓ located verbatim in the source · title, characters 0–161 · show in text ↑
e3

“Combined treatment of CAL and GA partially reversed the inhibitive effects of CAL on respiration and growth”

✓ located verbatim in the source · abstract, characters 361–468 · show in text ↑
Associationobservedoriginal resultno effectmeasured

Claim · model-written summary

Proteinase activity did not correlate with respiration or either amylase activity.

Gene
α-or β-amylasee0t0
Outcome
proteinase activitye0t0
Direction
No correlationse0t0
Qualifier
respiration or α-or β-amylase activitiese0t0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“No correlations were found between proteinase activity and respiration or α-or β-amylase activities.”

✓ located verbatim in the source · abstract, characters 531–631 · show in text ↑
t0

“MECHANISM OF ACTION OF GIBBERELLIC ACID: INHIBITION OF α-AMYLASE DEVELOPMENT DURING GERMINATION OF BARLEY BY CHLORAMPHENICOL AND ITS REVERSAL BY GIBBERELLIC ACID”

✓ located verbatim in the source · title, characters 0–161 · show in text ↑
Mechanismhypothesisoriginal resultbasis n/a

Claim · model-written summary

Authors suggest α-amylase is synthesized during germination and GA accelerates this synthesis.

Treatment
GAe0t0
Gene
α-amylasee0t0
Outcome
α-amylase is synthesized during germinatione0t0
Direction
acceleratese0t0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Hordeum vulgare
Hordeum vulgare · TaxonJackyM59 · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“It is suggested that α-amylase is synthesized during germination and that GA accelerates this synthesis.”

✓ located verbatim in the source · abstract, characters 742–846 · show in text ↑
t0

“MECHANISM OF ACTION OF GIBBERELLIC ACID: INHIBITION OF α-AMYLASE DEVELOPMENT DURING GERMINATION OF BARLEY BY CHLORAMPHENICOL AND ITS REVERSAL BY GIBBERELLIC ACID”

✓ located verbatim in the source · title, characters 0–161 · show in text ↑

Gibberellic acid · physiology: enzyme activity · Hordeum vulgare (barley)Positive and negative results

17 papers: 14 positive, 3 negative or no effect

13 papers in the same direction as this paper · 3 in the opposite direction

  1. no effect
  2. negative
    GA with calcium chloride reduced xylanase activity versus GA or GA/ABA.

    Time Course for the Development of Enzymes in Barley1 · 2007 · compared with treatment with GA or with GA/ABA mixtures

    Opposite direction

  3. no effect

13 papers more in this group

See all 17 papers →

Gibberellic acid · physiology: respiration · Hordeum vulgare (barley)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive

1 paper more in this group

See all 5 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.