Abstract
ABSTRACT Ultraviolet‐B‐induced lesions and their photorepair in nuclear and chloroplast DNA of spinach ( Spinacia oleracea L.) leaves were examined with two photoproducts, cyclobutane pyrimidine dimers (CPD) and pyrimidine (6‐4) pyrimidinone photoproducts (6‐4PP). These photoproducts were induced both in nuclear and chloroplast DNA by UVB irradiation and could be detected by enzyme‐linked immunosorbent assay using their respective monoclonal antibodies. Formation of CPD was greater in nuclear DNA than in chloroplast DNA (about 10 to 7), whereas 6‐4PP formation was comparable in both DNA. On subsequent exposure of leaves to blue/UVA after UVB irradiation, photorepair of CPD and 6‐4PP occurred in nuclear DNA but not in chloroplast DNA. When isolated chloroplasts were irradiated with UVB, CPD was also induced in their DNA. But photorepair of CPD did not occur in them by subsequent exposure to blue/UVA, suggesting that no photorepair system operates in chloroplasts.
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Claims 6
Claim · model-written summary
UVB induced CPD and 6-4PP in both nuclear and chloroplast DNA.
- Taxon
- Spinacia oleracea L.
Spinacia oleraceae2 - Organ / stage
- nuclear and chloroplast DNAe0
- Treatment
- UVB irradiatione0
- Outcome
- photoproductse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These photoproducts were induced both in nuclear and chloroplast DNA by UVB irradiation”
e1“cyclobutane pyrimidine dimers (CPD) and pyrimidine (6‐4) pyrimidinone photoproducts (6‐4PP)”
e2“Spinacia oleracea L.”
Claim · model-written summary
CPD formation was greater in nuclear than chloroplast DNA at about 10 to 7.
- Taxon
- Spinacia oleracea L.Spinacia oleraceae1
- Outcome
- Formation of CPDe0
- Direction
- greatere0
- Value
- about 10 to 7e0
- Comparator
- chloroplast DNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Formation of CPD was greater in nuclear DNA than in chloroplast DNA (about 10 to 7)”
e1“Spinacia oleracea L.”
Claim · model-written summary
6-4PP formation was comparable in nuclear and chloroplast DNA.
- Taxon
- Spinacia oleracea L.Spinacia oleraceae1
- Outcome
- 6‐4PP formatione0
- Direction
- comparablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“6‐4PP formation was comparable in both DNA”
e1“Spinacia oleracea L.”
Claim · model-written summary
Blue/UVA exposure after UVB repaired nuclear CPD and 6-4PP but not chloroplast lesions.
- Taxon
- Spinacia oleracea L.Spinacia oleraceae1
- Treatment
- exposure of leaves to blue/UVA after UVB irradiatione0
- Outcome
- photorepair of CPD and 6‐4PPe0
- Comparator
- chloroplast DNAe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“On subsequent exposure of leaves to blue/UVA after UVB irradiation, photorepair of CPD and 6‐4PP occurred in nuclear DNA but not in chloroplast DNA”
e1“Spinacia oleracea L.”
Claim · model-written summary
UVB induced CPD in DNA of isolated chloroplasts.
- Taxon
- Spinacia oleracea L.Spinacia oleraceae1
- Organ / stage
- isolated chloroplastse0
- Treatment
- irradiated with UVBe0
- Outcome
- CPD was also induced in their DNAe0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When isolated chloroplasts were irradiated with UVB, CPD was also induced in their DNA”
e1“Spinacia oleracea L.”
Claim · model-written summary
Blue/UVA did not photorepair CPD in isolated chloroplasts.
- Taxon
- Spinacia oleracea L.Spinacia oleraceae2
- Organ / stage
- isolated chloroplastse1
- Treatment
- subsequent exposure to blue/UVAe0
- Outcome
- photorepair of CPD did not occure0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“photorepair of CPD did not occur in them by subsequent exposure to blue/UVA”
e1“isolated chloroplasts were irradiated with UVB”
e2“Spinacia oleracea L.”
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See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.