Abstract
Digestion of spinach chloroplasts with pancreatic lipase or trypsin effectively uncoupled electron transport. Continued digestion led to inhibition of saturated rates of Hill reaction activity and a decrease in quantum yield. Irradiation with ultraviolet light decreased the quantum yield and inhibited Hill activity, but did not uncouple. Ascorbate-dichlorophenol-indophenol-mediated reduction of nicotinamide adenine dinucleotide phosphate was not appreciably inhibited by treatment with either of the enzymes or by ultraviolet irradiation.Carbonylcyanide m-chlorophenylhydrazone became a potent inhibitor of electron transport after trypsin treatment of chloroplasts. It also inhibited, rather than uncoupled, electron transport in glutaraldehyde-fixed chloroplasts. No other uncouplers tested showed these effects. Glutaraldehyde fixation of chloroplasts also greatly reduced the inhibitory effects of lipase and trypsin digestion but not the inhibition by ultraviolet irradiation.The inhibitory effects of trypsin and pancreatic lipase, and probably ultraviolet irradiation as well, appear to be due to a general breakdown of the membrane structure rather than inactivation of specific sites in the electron transport chain.
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Claims 9
Claim · model-written summary
Pancreatic lipase uncoupled electron transport in spinach chloroplasts.
- Taxon
- spinach
Spinacia oleraceae0 - Treatment
- pancreatic lipasee0
- Outcome
- uncoupled electron transporte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Digestion of spinach chloroplasts with pancreatic lipase or trypsin effectively uncoupled electron transport”
Claim · model-written summary
Trypsin uncoupled electron transport in spinach chloroplasts.
- Taxon
- spinachSpinacia oleraceae0
- Treatment
- trypsine0
- Outcome
- uncoupled electron transporte0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Digestion of spinach chloroplasts with pancreatic lipase or trypsin effectively uncoupled electron transport”
Claim · model-written summary
Continued digestion inhibited saturated Hill reaction rates and decreased quantum yield.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- Continued digestione0
- Outcome
- saturated rates of Hill reaction activity and a decrease in quantum yielde0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Continued digestion led to inhibition of saturated rates of Hill reaction activity and a decrease in quantum yield”
t0“spinach chloroplasts”
Claim · model-written summary
Ultraviolet irradiation decreased quantum yield and inhibited Hill activity without uncoupling.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- ultraviolet lighte0
- Outcome
- quantum yield and inhibited Hill activitye0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Irradiation with ultraviolet light decreased the quantum yield and inhibited Hill activity, but did not uncouple”
t0“spinach chloroplasts”
Claim · model-written summary
Enzyme treatments and ultraviolet irradiation did not appreciably inhibit NADP reduction.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- either of the enzymes or by ultraviolet irradiatione0
- Outcome
- reduction of nicotinamide adenine dinucleotide phosphatee0
- Direction
- not appreciably inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“reduction of nicotinamide adenine dinucleotide phosphate was not appreciably inhibited by treatment with either of the enzymes or by ultraviolet irradiation”
t0“spinach chloroplasts”
Claim · model-written summary
Carbonylcyanide m-chlorophenylhydrazone became a potent electron-transport inhibitor after trypsin treatment.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- Carbonylcyanide m-chlorophenylhydrazonee0
- Outcome
- inhibitor of electron transporte0
- Qualifier
- after trypsin treatmente0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Carbonylcyanide m-chlorophenylhydrazone became a potent inhibitor of electron transport after trypsin treatment of chloroplasts”
t0“spinach chloroplasts”
Claim · model-written summary
Carbonylcyanide m-chlorophenylhydrazone inhibited electron transport in fixed chloroplasts.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- glutaraldehyde-fixed chloroplastse0
- Outcome
- electron transporte0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“It also inhibited, rather than uncoupled, electron transport in glutaraldehyde-fixed chloroplasts”
t0“spinach chloroplasts”
Claim · model-written summary
Glutaraldehyde fixation greatly reduced inhibitory effects of lipase and trypsin digestion.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- Glutaraldehyde fixatione0
- Outcome
- inhibitory effects of lipase and trypsin digestione0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Glutaraldehyde fixation of chloroplasts also greatly reduced the inhibitory effects of lipase and trypsin digestion”
t0“spinach chloroplasts”
Claim · model-written summary
Glutaraldehyde fixation did not reduce inhibition by ultraviolet irradiation.
- Taxon
- spinachSpinacia oleraceat0
- Treatment
- ultraviolet irradiatione0
- Outcome
- inhibitione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but not the inhibition by ultraviolet irradiation”
t0“spinach chloroplasts”
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