Paper · 1974 · Physiologia Plantarum

Action Spectrum for Photoactivation of the Water‐splitting System in Plastids of Intermittently Illuminated Wheat Leaves

YORINAO INOUE; YOSHICHIKA KOBAYASHI; EMIKO SAKAMOTO; et al.

7
claims
12
verbatim quotes
25
citations
1
research areas
—
Not open access

Abstract

Abstract The chloroplasts from wheat leaves developed under intermittent illumination (1 ms light + 12 min dark) were able to photoreduce DPIP with DPC as electron donor but unable to photoreduce DPIP with water as electron donor. On exposure of these leaves to continuous light, the Hill activity with water as electron donor was rapidly induced. The photoactivation was sensitive to the treatment with DCMU prior to exposure to continuous light. The action spectrum for the photoactivation showed a sharp band at 680 nm with a distinct shoulder at 650 nm, and was similar to the absorption spectrum of photosytem‐2 particles. These data suggest that the electron transfer driven by photosystem 2 is essential for the activation of the water‐splitting system in the chloroplasts of intermittently illuminated leaves.

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Claims 7

Observationobservedoriginal resultmeasured

Claim · model-written summary

Chloroplasts from wheat leaves grown under intermittent illumination could photoreduce DPIP with DPC as electron donor.

Treatment
intermittent illumination (1 ms light + 12 min dark)e0
Outcome
photoreduce DPIP with DPC as electron donore0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The chloroplasts from wheat leaves developed under intermittent illumination (1 ms light + 12 min dark) were able to photoreduce DPIP with DPC as electron donor”

✓ located verbatim in the source · abstract, characters 9–169 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Chloroplasts from wheat leaves grown under intermittent illumination were unable to photoreduce DPIP with water as electron donor.

Treatment
intermittent illumination (1 ms light + 12 min dark)e1
Outcome
unable to photoreduce DPIP with water as electron donore1

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e1

“The chloroplasts from wheat leaves developed under intermittent illumination (1 ms light + 12 min dark) were able to photoreduce DPIP with DPC as electron donor but unable to photoreduce DPIP with water as electron donor”

✓ located verbatim in the source · abstract, characters 9–229 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Exposure of the intermittently illuminated wheat leaves to continuous light rapidly induced Hill activity with water as electron donor.

Treatment
continuous lighte2
Outcome
Hill activity with water as electron donore2
Direction
inducede2

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e2

“On exposure of these leaves to continuous light, the Hill activity with water as electron donor was rapidly induced”

✓ located verbatim in the source · abstract, characters 231–346 · show in text ↑
t0

“Wheat Leaves”

✓ located verbatim in the source · title, characters 108–120 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

The photoactivation was sensitive to DCMU treatment given before exposure to continuous light.

Treatment
DCMUe3
Outcome
photoactivatione3

Not reportedComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e3

“The photoactivation was sensitive to the treatment with DCMU prior to exposure to continuous light”

✓ located verbatim in the source · abstract, characters 348–446 · show in text ↑
t0

“Wheat Leaves”

✓ located verbatim in the source · title, characters 108–120 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The action spectrum for the photoactivation showed a sharp band at 680 nm with a distinct shoulder at 650 nm.

Outcome
action spectrum for the photoactivatione4
Value
680 nme4
Qualifier
distinct shoulder at 650 nme4

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e4

“The action spectrum for the photoactivation showed a sharp band at 680 nm with a distinct shoulder at 650 nm”

✓ located verbatim in the source · abstract, characters 448–556 · show in text ↑
t0

“Wheat Leaves”

✓ located verbatim in the source · title, characters 108–120 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The action spectrum was similar to the absorption spectrum of photosystem 2 particles.

Outcome
similar to the absorption spectrume5

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e5

“was similar to the absorption spectrum of photosytem‐2 particles”

✓ located verbatim in the source · abstract, characters 562–626 · show in text ↑
t0

“Wheat Leaves”

✓ located verbatim in the source · title, characters 108–120 · show in text ↑
Mechanismauthor interpretationoriginal resultbasis n/a

Claim · model-written summary

The authors suggest that photosystem 2-driven electron transfer is essential for activating the water-splitting system in chloroplasts of intermittently illuminated leaves.

Outcome
activation of the water‐splitting systeme6
Qualifier
electron transfer driven by photosystem 2 is essentiale6

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Triticum aestivum
Triticum aestivum · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e6

“electron transfer driven by photosystem 2 is essential for the activation of the water‐splitting system in the chloroplasts of intermittently illuminated leaves”

✓ located verbatim in the source · abstract, characters 656–816 · show in text ↑
t0

“Wheat Leaves”

✓ located verbatim in the source · title, characters 108–120 · show in text ↑

light · molecular: metabolites · Triticum aestivum (wheat)Positive and negative results

29 papers: 14 positive, 15 negative or no effect

13 papers in the same direction as this paper · 15 in the opposite direction

  1. no effect
  2. no effect
  3. negative

25 papers more in this group

See all 29 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.