Abstract
A tomato (Lycopersicon esculentum Mill.) monodehydroascorbate reductase gene (LeMDAR) was isolated. The LeMDAR-green fluorescence protein (GFP) fusion protein was targeted to chloroplast in Arabidopsis mesophyll protoplast. RNA and protein gel blot analyses confirmed that the sense- and antisense- LeMDAR were integrated into the tomato genome. The MDAR activities and the levels of reduced ascorbate (AsA) were markedly increased in sense transgenic lines and decreased in antisense transgenic lines compared with wild-type (WT) plants. Under low and high temperature stresses, the sense transgenic plants showed lower level of hydrogen peroxide (H(2)O(2)), lower thiobarbituric acid reactive substance (TBARS) content, higher net photosynthetic rate (P(n)), higher maximal photochemical efficiency of PSII (F(v)/F(m)) and fresh weight compared with WT plants. The oxidizable P700 decreased more obviously in WT and antisense plants than that in sense plants at chilling temperature under low irradiance. Furthermore, the sense transgenic plants exhibited significantly lower H(2)O(2) level, higher ascorbate peroxidase (APX) activity, greater P(n) and F(v)/F(m) under methyl viologen (MV)-mediated oxidative stresses. These results indicated that overexpression of chloroplastic MDAR played an important role in alleviating photoinhibition of PSI and PSII and enhancing the tolerance to various abiotic stresses by elevating AsA level.
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Claims 15
Claim · model-written summary
Tomato LeMDAR was isolated.
- Taxon
- tomato
Solanum lycopersicumt0 - Gene
- LeMDARe1
- Outcome
- was isolatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“A tomato (Lycopersicon esculentum Mill.) monodehydroascorbate reductase gene (LeMDAR) was isolated”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
LeMDAR-GFP localized to chloroplasts in Arabidopsis mesophyll protoplasts.
- Taxon
- Arabidopsis
Arabidopsist0 - Organ / stage
- mesophyll protoplaste1
- Gene
- LeMDAR-green fluorescence protein (GFP)e1
- Outcome
- targeted to chloroplaste1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“LeMDAR-green fluorescence protein (GFP) fusion protein was targeted to chloroplast in Arabidopsis mesophyll protoplast”
t0“Arabidopsis mesophyll protoplast”
Claim · model-written summary
Sense LeMDAR tomato lines had increased MDAR activity versus wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- sense transgenic linese1
- Outcome
- MDAR activitiese1
- Direction
- increasede1
- Comparator
- wild-type (WT) plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“MDAR activities and the levels of reduced ascorbate (AsA) were markedly increased in sense transgenic lines and decreased in antisense transgenic lines compared with wild-type (WT) plants”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Sense LeMDAR tomato lines had increased reduced ascorbate versus wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- sense transgenic linese1
- Outcome
- levels of reduced ascorbate (AsA)e1
- Direction
- increasede1
- Comparator
- wild-type (WT) plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“MDAR activities and the levels of reduced ascorbate (AsA) were markedly increased in sense transgenic lines and decreased in antisense transgenic lines compared with wild-type (WT) plants”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Antisense LeMDAR tomato lines had decreased MDAR activity versus wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- antisense transgenic linese1
- Outcome
- MDAR activitiese1
- Direction
- decreasede1
- Comparator
- wild-type (WT) plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“MDAR activities and the levels of reduced ascorbate (AsA) were markedly increased in sense transgenic lines and decreased in antisense transgenic lines compared with wild-type (WT) plants”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Antisense LeMDAR tomato lines had decreased reduced ascorbate versus wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- antisense transgenic linese1
- Outcome
- levels of reduced ascorbate (AsA)e1
- Direction
- decreasede1
- Comparator
- wild-type (WT) plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“MDAR activities and the levels of reduced ascorbate (AsA) were markedly increased in sense transgenic lines and decreased in antisense transgenic lines compared with wild-type (WT) plants”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Sense lines had less hydrogen peroxide under low and high temperature stress than wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- sense transgenic plantse1
- Outcome
- level of hydrogen peroxide (H(2)O(2))e1
- Direction
- lowere1
- Qualifier
- low and high temperature stressese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Under low and high temperature stresses, the sense transgenic plants showed lower level of hydrogen peroxide (H(2)O(2))”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Sense lines had less TBARS under temperature stress than wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- thiobarbituric acid reactive substance (TBARS) contente1
- Direction
- lowere1
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“lower thiobarbituric acid reactive substance (TBARS) content”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Sense lines had higher net photosynthetic rate and PSII efficiency under temperature stress than wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- net photosynthetic rate (P(n)), higher maximal photochemical efficiency of PSII (F(v)/F(m))e1
- Direction
- highere1
- Comparator
- WT plantse1
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“higher net photosynthetic rate (P(n)), higher maximal photochemical efficiency of PSII (F(v)/F(m)) and fresh weight compared with WT plants”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Sense lines had higher fresh weight under temperature stress than wild type.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- fresh weighte1
- Direction
- highere1
- Comparator
- WT plantse1
Not reportedTreatment
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“higher maximal photochemical efficiency of PSII (F(v)/F(m)) and fresh weight compared with WT plants”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Oxidizable P700 fell more in wild type and antisense than sense plants during chilling under low irradiance.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- chilling temperature under low irradiancee1
- Outcome
- oxidizable P700e1
- Direction
- decreasede1
- Qualifier
- WT and antisense plantse1
- Comparator
- sense plantse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“oxidizable P700 decreased more obviously in WT and antisense plants than that in sense plants at chilling temperature under low irradiance”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Sense lines had significantly lower hydrogen peroxide under methyl viologen stress.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- sense transgenic plantse1
- Outcome
- H(2)O(2) levele1
- Direction
- lowere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“sense transgenic plants exhibited significantly lower H(2)O(2) level”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Sense lines had higher APX activity under methyl viologen stress.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- ascorbate peroxidase (APX) activitye1
- Direction
- highere1
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“higher ascorbate peroxidase (APX) activity”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Sense lines had greater photosynthetic rate and PSII efficiency under methyl viologen stress.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- methyl viologen (MV)-mediated oxidative stressese1
- Outcome
- P(n) and F(v)/F(m)e1
- Direction
- greatere1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“greater P(n) and F(v)/F(m) under methyl viologen (MV)-mediated oxidative stresses”
t0“A tomato (Lycopersicon esculentum Mill.)”
Claim · model-written summary
Authors infer chloroplastic MDAR overexpression alleviates PSI and PSII photoinhibition and boosts abiotic stress tolerance via AsA.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- chloroplastic MDARe1
- Compound
- AsAe1
- Outcome
- photoinhibition of PSI and PSII and enhancing the tolerance to various abiotic stressese1
- Qualifier
- by elevating AsA levele1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“overexpression of chloroplastic MDAR played an important role in alleviating photoinhibition of PSI and PSII and enhancing the tolerance to various abiotic stresses by elevating AsA level”
t0“A tomato (Lycopersicon esculentum Mill.)”
The same questions in other papers
transgenic · molecular: metabolites · Solanum lycopersicum (tomato)Positive and negative results
15 papers in the same direction as this paper · 9 in the opposite direction
- negative
- no effect
- negative
21 papers more in this group
See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.