Abstract
We have identified two major groups of pectin methylesterase (PME, EC 3.1.1.11) isoforms in various tissues of tomatoes (Lycopersicon esculentum). These two groups exhibited differential immuno-cross-reactivity with polyclonal antibodies raised against tomato fruit PME or flax callus PME and differences in their accumulation patterns in tissues of wild-type and transgenic tomato plants expressing a PME antisense gene. The group I isoforms with isoelectric points (pls) of 8.2, 8.4, and 8.5 are specific to fruit tissue, where they are the major forms of PME activity. The group II PME isoforms, with pl values of 9 and above, are observed in both vegetative and fruit tissues. The group I isoforms cross-react with polyclonal antibodies raised to a PME isoform purified from fruit, whereas the group II isoforms cross-react with antibodies to a PME purified from flax callus. Expression of a fruit-specific PME anti-sense gene impairs accumulation of the group I PME isoforms, with no apparent effect on the accumulation of the group II PME isoforms. The absence of any noticeable effects on growth and development of transgenic plants suggests that the group I PME isoforms are not involved in plant growth and development and may play a role under special circumstances such as cell separation during fruit ripening.
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Claims 10
Claim · model-written summary
Two major PME isoform groups were identified in tomato tissues.
- Taxon
- tomatoes (Lycopersicon esculentum)
Solanum lycopersicume0 - Outcome
- two major groups of pectin methylesterase (PME, EC 3.1.1.11) isoformse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified two major groups of pectin methylesterase (PME, EC 3.1.1.11) isoforms in various tissues of tomatoes (Lycopersicon esculentum)”
Claim · model-written summary
The two PME groups differed in antibody cross-reactivity and tissue accumulation patterns.
- Taxon
- tomatoSolanum lycopersicume0
- Gene
- PMEe0
- Outcome
- differential immuno-cross-reactivitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These two groups exhibited differential immuno-cross-reactivity with polyclonal antibodies raised against tomato fruit PME or flax callus PME and differences in their accumulation patterns in tissues of wild-type and transgenic tomato plants expressing a PME antisense gene.”
Claim · model-written summary
Group I isoforms have pIs of 8.2, 8.4 and 8.5 and are the major fruit PME activity forms.
- Taxon
- TomatoSolanum lycopersicumt0
- Organ / stage
- fruit tissuee0
- Gene
- group I isoformse0
- Outcome
- major forms of PME activitye0
- Value
- 8.2, 8.4, and 8.5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The group I isoforms with isoelectric points (pls) of 8.2, 8.4, and 8.5 are specific to fruit tissue, where they are the major forms of PME activity.”
t0“Tomato”
Claim · model-written summary
Group II PME isoforms occur in vegetative and fruit tissues and have pI at least 9.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- group II PME isoformse0
- Outcome
- observed in both vegetative and fruit tissuese0
- Value
- 9 and abovee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The group II PME isoforms, with pl values of 9 and above, are observed in both vegetative and fruit tissues.”
t0“Tomato”
Claim · model-written summary
Group I isoforms react with antibodies to fruit PME.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- group I isoformse0
- Outcome
- cross-react with polyclonal antibodiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The group I isoforms cross-react with polyclonal antibodies raised to a PME isoform purified from fruit”
t0“Tomato”
Claim · model-written summary
Group II isoforms react with antibodies to flax callus PME.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- group II isoformse0
- Outcome
- cross-react with antibodiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the group II isoforms cross-react with antibodies to a PME purified from flax callus”
t0“Tomato”
Claim · model-written summary
Fruit-specific PME antisense expression impairs group I isoform accumulation.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Expression of a fruit-specific PME anti-sense genee0
- Outcome
- accumulation of the group I PME isoformse0
- Direction
- impairse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of a fruit-specific PME anti-sense gene impairs accumulation of the group I PME isoforms”
t0“Tomato”
Claim · model-written summary
Fruit-specific PME antisense expression has no apparent effect on group II isoform accumulation.
- Taxon
- TomatoSolanum lycopersicumt0
- Treatment
- Expression of a fruit-specific PME anti-sense genee0
- Outcome
- accumulation of the group II PME isoformse0
- Direction
- no apparent effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of a fruit-specific PME anti-sense gene impairs accumulation of the group I PME isoforms, with no apparent effect on the accumulation of the group II PME isoforms.”
t0“Tomato”
Claim · model-written summary
Transgenic plants had no noticeable growth or developmental effects.
- Taxon
- TomatoSolanum lycopersicumt0
- Outcome
- growth and developmente0
- Direction
- absence of any noticeable effectse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The absence of any noticeable effects on growth and development of transgenic plants”
t0“Tomato”
Claim · model-written summary
The authors suggest group I isoforms may function in cell separation during fruit ripening.
- Taxon
- TomatoSolanum lycopersicumt0
- Gene
- group I PME isoformse0
- Outcome
- cell separation during fruit ripeninge0
- Qualifier
- may play a rolee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the group I PME isoforms are not involved in plant growth and development and may play a role under special circumstances such as cell separation during fruit ripening”
t0“Tomato”
The same questions in other papers
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See all 25 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.