Abstract
As a part of our effort to study the mode of action of abscisic acid (ABA) and its metabolites during seed germination, we have investigated the regulation of ABA metabolism in barley (Hordeum vulgare) aleurone layers and a few other plant tissues. The rate of conversion of [(3)H]ABA to [(3)H]phaseic acid (PA), the first stable metabolite of ABA, is enhanced by 2- to 5-fold in barley aleurone layers when the tissue is pretreated with ABA. However, the conversion of [(3)H]PA to [(3)H] dihydrophaseic acid (DPA), the next metabolite after PA, is not enhanced by pretreatment with either ABA or PA. The ABA enhancement of its own metabolism in barley aleurone layers is detectable with a pretreatment of ABA ranging from 10(-3) to 10(-4) molar. This apparent self-induction of ABA conversion to PA can be observed after the barley aleurone layers have been treated with 10(-5) molar ABA for as short as 2 hours, and is inhibited by the transcription inhibitor, cordycepin (3'-deoxyadenosine), or the translation inhibitor, cycloheximide. The self-induction of ABA conversion to PA also occurs in wheat aleurone layers, but not in other plant tissues that have been investigated, including corn root tips, barley embryos, barley, and soybean leaf discs. It is probably a phenomenon unique to the aleurone layers of some cereal grains. In view of the recent observations that ABA is able to induce new proteins in barley aleurone layers, we suggest that some of these ABA-induced proteins are involved in the conversion from ABA to PA in this tissue.
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Claims 7
Claim · model-written summary
The rate of conversion of [(3)H]ABA to [(3)H]phaseic acid was enhanced 2- to 5-fold in barley aleurone layers pretreated with ABA.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Organ / stage
- aleurone layerse0
- Treatment
- pretreated with ABAe0
- Outcome
- rate of conversion of [(3)H]ABA to [(3)H]phaseic acid (PA)e0
- Direction
- enhancede0
- Value
- 2- to 5-folde0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The rate of conversion of [(3)H]ABA to [(3)H]phaseic acid (PA), the first stable metabolite of ABA, is enhanced by 2- to 5-fold in barley aleurone layers when the tissue is pretreated with ABA”
Claim · model-written summary
The conversion of [(3)H]PA to [(3)H]dihydrophaseic acid was not enhanced by pretreatment with either ABA or PA.
- Taxon
- Hordeum vulgareHordeum vulgaree0
- Organ / stage
- aleurone layerse0
- Treatment
- pretreatment with either ABA or PAe1
- Outcome
- conversion of [(3)H]PA to [(3)H] dihydrophaseic acid (DPA)e1
- Direction
- not enhancede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley (Hordeum vulgare) aleurone layers”
e1“the conversion of [(3)H]PA to [(3)H] dihydrophaseic acid (DPA), the next metabolite after PA, is not enhanced by pretreatment with either ABA or PA”
Claim · model-written summary
ABA enhancement of its own metabolism in barley aleurone layers was detectable with ABA pretreatment ranging from 10(-3) to 10(-4) molar.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- aleurone layerse0
- Treatment
- pretreatment of ABA ranging from 10(-3) to 10(-4) molare0
- Outcome
- ABA enhancement of its own metabolisme0
- Direction
- enhancemente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The ABA enhancement of its own metabolism in barley aleurone layers is detectable with a pretreatment of ABA ranging from 10(-3) to 10(-4) molar”
Claim · model-written summary
Self-induction of ABA conversion to PA was observed after barley aleurone layers were treated with 10(-5) molar ABA for as short as 2 hours.
- Taxon
- barleyHordeum vulgaree0
- Organ / stage
- aleurone layerse0
- Treatment
- 10(-5) molar ABA for as short as 2 hourse0
- Outcome
- self-induction of ABA conversion to PAe0
- Direction
- self-inductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This apparent self-induction of ABA conversion to PA can be observed after the barley aleurone layers have been treated with 10(-5) molar ABA for as short as 2 hours”
Claim · model-written summary
Self-induction of ABA conversion to PA also occurs in wheat aleurone layers.
- Taxon
- wheat
Triticum aestivume0 - Organ / stage
- aleurone layerse0
- Outcome
- self-induction of ABA conversion to PAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The self-induction of ABA conversion to PA also occurs in wheat aleurone layers”
Claim · model-written summary
Self-induction of ABA conversion to PA did not occur in other tissues investigated, including corn root tips, barley embryos, barley and soybean leaf discs.
- Organ / stage
- corn root tips, barley embryos, barley, and soybean leaf discse0
- Outcome
- not in other plant tissuese0
Not reportedTaxon
Verbatim
e0“but not in other plant tissues that have been investigated, including corn root tips, barley embryos, barley, and soybean leaf discs”
Claim · model-written summary
The authors suggest that some ABA-induced proteins are involved in conversion of ABA to PA in barley aleurone layers.
- Taxon
- Hordeum vulgareHordeum vulgaree0
- Organ / stage
- aleurone layerse0
- Gene
- ABA-induced proteinse1
- Outcome
- conversion from ABA to PAe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“barley (Hordeum vulgare) aleurone layers”
e1“we suggest that some of these ABA-induced proteins are involved in the conversion from ABA to PA in this tissue”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- positive
- no effect
- negative
11 papers more in this group
See all 15 papers →phaseic acid · molecular: metabolites · Hordeum vulgare (barley)Results agree
1 paper in the same direction as this paper
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.