Abstract
[(35)S]Methionine labeling experiments showed that abscisic acid (ABA) induced the synthesis of at least 25 polypeptides in mature barley (Hordeum vulgare) aleurone cells. The polypeptides were not secreted. Whereas most of the proteins extracted from aleurone cells were coagulated by heating to 100 degrees C for 10 minutes, most of the ABA-induced polypeptides remained in solution (heat-stable). ABA had little effect on the spectrum of polypeptides that were synthesized and secreted by aleurone cells, and most of these secreted polypeptides were also heatstable. Coomassie blue staining of sodium dodecyl sulfate polyacrylamide gels indicated that ABA-induced polypeptides already occurred in high amounts in mature aleurone layers having accumulated during grain development. About 60% of the total protein extracted from mature aleurone was heat stable. Amino acid analyses of total preparations of heat-stable and heat-labile proteins showed that, compared to heat-labile proteins, heat-stable intracellular proteins were characterized by higher glutamic acid/glutamine (Glx) and glycine levels and lower levels of neutral amino acids. Secreted heat-stable proteins were rich in Glx and proline. The possibilities that the accumulation of the heat-stable polypeptides during grain development is controlled by ABA and that the function of these polypeptides is related to their abundance and extraordinary heat stability are considered.
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Claims 9
Claim · model-written summary
ABA induced at least 25 polypeptides in mature barley aleurone cells.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaree0 - Organ / stage
- aleurone cellse0
- Treatment
- abscisic acid (ABA)e0
- Outcome
- synthesis of at least 25 polypeptidese0
- Direction
- inducede0
- Value
- at least 25 polypeptidese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“abscisic acid (ABA) induced the synthesis of at least 25 polypeptides in mature barley (Hordeum vulgare) aleurone cells.”
Claim · model-written summary
The ABA-induced polypeptides were not secreted.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- not secretede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The polypeptides were not secreted.”
t0“Barley Aleurone Cells”
Claim · model-written summary
Most ABA-induced polypeptides remained soluble after heating.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- remained in solution (heat-stable)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most of the ABA-induced polypeptides remained in solution (heat-stable).”
t0“Barley Aleurone Cells”
Claim · model-written summary
ABA had little effect on synthesized and secreted polypeptide spectrum.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- ABAe0
- Outcome
- spectrum of polypeptidese0
- Direction
- little effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA had little effect on the spectrum of polypeptides that were synthesized and secreted by aleurone cells”
t0“Barley Aleurone Cells”
Claim · model-written summary
Most secreted polypeptides were heat stable.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- heatstablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most of these secreted polypeptides were also heatstable.”
t0“Barley Aleurone Cells”
Claim · model-written summary
ABA-induced polypeptides were already abundant in mature aleurone layers.
- Taxon
- barleyHordeum vulgaret0
- Organ / stage
- mature aleurone layerse0
- Outcome
- ABA-induced polypeptides already occurred in high amountse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA-induced polypeptides already occurred in high amounts in mature aleurone layers having accumulated during grain development.”
t0“Barley Aleurone Cells”
Claim · model-written summary
About 60% of protein extracted from mature aleurone was heat stable.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- total protein extracted from mature aleurone was heat stablee0
- Value
- About 60%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“About 60% of the total protein extracted from mature aleurone was heat stable.”
t0“Barley Aleurone Cells”
Claim · model-written summary
Heat-stable intracellular proteins had higher Glx and glycine and lower neutral amino acids than heat-labile proteins.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- higher glutamic acid/glutamine (Glx) and glycine levels and lower levels of neutral amino acidse0
- Comparator
- heat-labile proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“compared to heat-labile proteins, heat-stable intracellular proteins were characterized by higher glutamic acid/glutamine (Glx) and glycine levels and lower levels of neutral amino acids.”
t0“Barley Aleurone Cells”
Claim · model-written summary
Secreted heat-stable proteins were rich in Glx and proline.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- rich in Glx and prolinee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Secreted heat-stable proteins were rich in Glx and proline.”
t0“Barley Aleurone Cells”
The same questions in other papers
(+)-abscisic acid · molecular: metabolites · Hordeum vulgare (barley)Positive and negative results
7 papers in the same direction as this paper · 7 in the opposite direction
- positive
- no effect
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.