Abstract
A highly oxidative stress-tolerant japonica rice line was isolated by T-DNA insertion mutation followed by screening in the presence of 50 mM H(2)O(2). The T-DNA insertion was mapped to locus Os09g0547500, the gene product of which was annotated as lysine decarboxylase-like protein (GenBank accession No. AK062595). We termed this gene OsLDC-like 1, for Oryza sativa lysine decarboxylase-like 1. The insertion site was in the second exon and resulted in a 27 amino acid N-terminal deletion. Despite this defect in OsLDC-like 1, the mutant line exhibited enhanced accumulation of the polyamines (PAs) putrescine, spermidine, and spermine under conditions of oxidative stress. The generation of reactive oxygen species (ROS) in the mutant line was assessed by qRT-PCR analysis of NADPH oxidase (RbohD and RbohF), and by DCFH-DA staining. Cellular levels of ROS in osldc-like 1 leaves were significantly lower than those in the wild-type (WT) rice after exposure to oxidative, high salt and acid stresses. Exogenously-applied PAs such as spermidine and spermine significantly inhibited the stress-induced accumulation of ROS and cell damage in WT leaves. Additionally, the activities of ROS-detoxifying enzymes were increased in the homozygous mutant line in the presence or absence of H(2)O(2). Thus, mutation of OsLDC-like 1 conferred an oxidative stress-tolerant phenotype. These results suggest that increased cellular PA levels have a physiological role in preventing stress-induced ROS and ethylene accumulation and the resultant cell damage.
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Claims 6
Claim · model-written summary
The OsLDC-like 1 mutant line accumulated more polyamines under oxidative stress.
- Taxon
- Oryza sativa
Oryza sativat0 - Material
- mutant linee1
- Treatment
- oxidative stresse1
- Outcome
- polyamines (PAs) putrescine, spermidine, and sperminee1
- Direction
- enhancede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e1“the mutant line exhibited enhanced accumulation of the polyamines (PAs) putrescine, spermidine, and spermine under conditions of oxidative stress”
Claim · model-written summary
Cellular ROS levels in osldc-like 1 leaves were significantly lower than in wild-type rice after oxidative, high salt and acid stresses.
- Taxon
- Oryza sativaOryza sativat0
- Organ / stage
- leavese2
- Treatment
- oxidative, high salt and acid stressese2
- Gene
- osldc-like 1e2
- Outcome
- Cellular levels of ROSe2
- Direction
- lowere2
- Comparator
- wild-type (WT) ricee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e2“Cellular levels of ROS in osldc-like 1 leaves were significantly lower than those in the wild-type (WT) rice after exposure to oxidative, high salt and acid stresses”
Claim · model-written summary
Exogenously applied spermidine and spermine significantly inhibited stress-induced ROS accumulation and cell damage in WT rice leaves.
- Taxon
- Oryza sativaOryza sativat0
- Organ / stage
- leavese3
- Treatment
- Exogenously-applied PAs such as spermidine and sperminee3
- Outcome
- stress-induced accumulation of ROS and cell damagee3
- Direction
- inhibitede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e3“Exogenously-applied PAs such as spermidine and spermine significantly inhibited the stress-induced accumulation of ROS and cell damage in WT leaves”
Claim · model-written summary
Activities of ROS-detoxifying enzymes were higher in the homozygous mutant line, with or without H2O2.
- Taxon
- Oryza sativaOryza sativat0
- Material
- homozygous mutant linee4
- Treatment
- H(2)O(2)e4
- Outcome
- activities of ROS-detoxifying enzymese4
- Direction
- increasede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e4“the activities of ROS-detoxifying enzymes were increased in the homozygous mutant line in the presence or absence of H(2)O(2)”
Claim · model-written summary
Mutation of OsLDC-like 1 conferred an oxidative stress-tolerant phenotype in rice.
- Taxon
- Oryza sativaOryza sativat0
- Treatment
- mutation of OsLDC-like 1e5
- Gene
- OsLDC-like 1e5
- Outcome
- oxidative stress-tolerant phenotypee5
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e5“mutation of OsLDC-like 1 conferred an oxidative stress-tolerant phenotype”
Claim · model-written summary
The authors suggest that increased cellular polyamine levels play a physiological role in preventing stress-induced ROS and ethylene accumulation.
- Taxon
- Oryza sativaOryza sativat0
- Compound
- PAe6
- Outcome
- stress-induced ROS and ethylene accumulatione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa”
e6“increased cellular PA levels have a physiological role in preventing stress-induced ROS and ethylene accumulation”
The same questions in other papers
loss of function · physiology: oxidative · Oryza sativa (rice)Positive and negative results
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