Abstract
Iron toxicity is one of the most widely-spread mineral disorders in anaerobic soils, but the tolerance mechanisms in plants are poorly understood. Here we characterize the involvement of a rice potassium ion channel gene, OsAKT1, in Fe toxic conditions. Two knock-down lines of OsAKT1 together with azygous lines were investigated. Mutant lines did not differ from azygous lines regarding plant growth, gas exchange rate or chlorophyll fluorescence in control conditions. However, loss-of-function of OsAKT1 increased the sensitivity to excess Fe regarding leaf bronzing symptoms, reactive oxygen species generation, leaf spectral reflectance indices, and chlorophyll fluorescence. Fe toxicity leads to largely reduced uptake of other nutrients into shoots, which illustrates the complexity of Fe stress related to multiple mineral disorders. Less potassium uptake in the mutants compared to azygous lines co-occurred with higher amounts of Fe accumulated in the shoot tissues but not in the roots. These results were consistent with a higher level of Fe loaded into the xylem sap of mutants compared to azygous lines in the early phase of Fe toxicity. In conclusion, OsAKT1 is crucial for the tolerance of rice against Fe toxicity as K homeostasis affects Fe translocation from root to shoot.
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Claims 10
Claim · model-written summary
OsAKT1 knock down did not alter growth, gas exchange or fluorescence under control conditions.
- Taxon
- Rice Plants
Oryza sativat0 - Material
- Mutant linese0
- Outcome
- plant growth, gas exchange rate or chlorophyll fluorescencee0
- Direction
- did not differe0
- Comparator
- azygous linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mutant lines did not differ from azygous lines regarding plant growth, gas exchange rate or chlorophyll fluorescence in control conditions.”
t0“Rice Plants”
Claim · model-written summary
OsAKT1 loss increased leaf bronzing sensitivity under excess Fe.
- Taxon
- Rice PlantsOryza sativat0
- Treatment
- loss-of-function of OsAKT1e0
- Gene
- OsAKT1e0
- Outcome
- leaf bronzing symptomse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss-of-function of OsAKT1 increased the sensitivity to excess Fe regarding leaf bronzing symptoms, reactive oxygen species generation, leaf spectral reflectance indices, and chlorophyll fluorescence.”
t0“Rice Plants”
Claim · model-written summary
OsAKT1 loss increased reactive oxygen species generation sensitivity under excess Fe.
- Taxon
- Rice PlantsOryza sativat0
- Treatment
- loss-of-function of OsAKT1e0
- Gene
- OsAKT1e0
- Outcome
- reactive oxygen species generatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss-of-function of OsAKT1 increased the sensitivity to excess Fe regarding leaf bronzing symptoms, reactive oxygen species generation, leaf spectral reflectance indices, and chlorophyll fluorescence.”
t0“Rice Plants”
Claim · model-written summary
OsAKT1 loss increased sensitivity measured by leaf reflectance and chlorophyll fluorescence.
- Taxon
- Rice PlantsOryza sativat0
- Treatment
- loss-of-function of OsAKT1e0
- Gene
- OsAKT1e0
- Outcome
- leaf spectral reflectance indices, and chlorophyll fluorescencee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“loss-of-function of OsAKT1 increased the sensitivity to excess Fe regarding leaf bronzing symptoms, reactive oxygen species generation, leaf spectral reflectance indices, and chlorophyll fluorescence.”
t0“Rice Plants”
Claim · model-written summary
Fe toxicity reduced uptake of other nutrients into shoots.
- Taxon
- Rice PlantsOryza sativat0
- Outcome
- uptake of other nutrients into shootse0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fe toxicity leads to largely reduced uptake of other nutrients into shoots”
t0“Rice Plants”
Claim · model-written summary
Mutants had less potassium uptake than azygous lines.
- Taxon
- Rice PlantsOryza sativat0
- Material
- mutantse0
- Outcome
- potassium uptakee0
- Direction
- Lesse0
- Comparator
- azygous linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Less potassium uptake in the mutants compared to azygous lines”
t0“Rice Plants”
Claim · model-written summary
Mutants accumulated more Fe in shoots than azygous lines.
- Taxon
- Rice PlantsOryza sativat0
- Organ / stage
- shoot tissuese0
- Outcome
- Fe accumulated in the shoot tissuese0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher amounts of Fe accumulated in the shoot tissues but not in the roots.”
t0“Rice Plants”
Claim · model-written summary
Fe accumulation was not higher in mutant roots.
- Taxon
- Rice PlantsOryza sativat0
- Organ / stage
- rootse0
- Outcome
- Fe accumulated in the shoot tissues but not in the rootse0
- Direction
- note0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“higher amounts of Fe accumulated in the shoot tissues but not in the roots.”
t0“Rice Plants”
Claim · model-written summary
Mutants had more Fe loaded into xylem sap early in toxicity.
- Taxon
- Rice PlantsOryza sativat0
- Material
- mutantse0
- Outcome
- Fe loaded into the xylem sape0
- Direction
- highere0
- Comparator
- azygous linese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a higher level of Fe loaded into the xylem sap of mutants compared to azygous lines in the early phase of Fe toxicity.”
t0“Rice Plants”
Claim · model-written summary
Authors infer that OsAKT1 supports Fe tolerance through K homeostasis and Fe translocation.
- Taxon
- riceOryza sativae0
- Gene
- OsAKT1e0
- Outcome
- tolerance of rice against Fe toxicitye0
- Qualifier
- K homeostasis affects Fe translocation from root to shoote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsAKT1 is crucial for the tolerance of rice against Fe toxicity as K homeostasis affects Fe translocation from root to shoot.”
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