Abstract
Abstract Glutaredoxins (GRXs) modulate redox-dependent signaling pathways and have emerged as key mediators in plant responses to environmental stimuli. Here we report that RNAi-mediated suppression of Oryza sativa GRXS17 ( OsGRXS17 ) improved drought tolerance in rice. Gene expression studies showed that OsGRXS17 was present throughout the plant and that transcript abundance increased in response to drought stress and abscisic acid (ABA) treatment. Localization studies, utilizing GFP-OsGRXS17 fusion proteins, indicated that OsGRXS17 resides in both the cytoplasm and the nuclear envelope. Under drought stress conditions, rice plants with reduced OsGRXS17 expression showed lower rates of water loss and stomatal conductance, higher relative water content, and enhanced survival compared to wild-type controls. Further characterization of the OsGRXS17 down-regulated plants revealed an elevation in H 2 O 2 production within the guard cells, increased sensitivity to ABA, and a reduction in stomatal apertures. The findings demonstrate a critical link between OsGRXS17, the modulation of guard cell H 2 O 2 concentrations, and stomatal closure, expanding our understanding of the mechanisms governing plant responses to drought.
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Claims 11
Claim · model-written summary
Suppression of OsGRXS17 improved drought tolerance in rice.
- Taxon
- rice
Oryza sativae0 - Treatment
- RNAi-mediated suppression of Oryza sativa GRXS17e0
- Gene
- OsGRXS17e0
- Outcome
- drought tolerancee0
- Direction
- improvede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RNAi-mediated suppression of Oryza sativa GRXS17 ( OsGRXS17 ) improved drought tolerance in rice”
Claim · model-written summary
OsGRXS17 transcripts were present throughout the rice plant.
- Taxon
- riceOryza sativat0
- Gene
- OsGRXS17e0
- Outcome
- present throughout the plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsGRXS17 was present throughout the plant”
t0“rice”
Claim · model-written summary
Drought stress increased OsGRXS17 transcript abundance.
- Taxon
- riceOryza sativat0
- Treatment
- drought stresse0
- Gene
- OsGRXS17e1
- Outcome
- transcript abundancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcript abundance increased in response to drought stress”
e1“OsGRXS17 was present throughout the plant”
t0“rice”
Claim · model-written summary
ABA treatment increased OsGRXS17 transcript abundance.
- Taxon
- riceOryza sativat0
- Treatment
- abscisic acid (ABA) treatmente0
- Gene
- OsGRXS17e1
- Outcome
- transcript abundancee0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcript abundance increased in response to drought stress and abscisic acid (ABA) treatment”
e1“OsGRXS17 was present throughout the plant”
t0“rice”
Claim · model-written summary
OsGRXS17 localized to the cytoplasm and nuclear envelope.
- Taxon
- riceOryza sativat0
- Gene
- OsGRXS17e0
- Outcome
- resides in both the cytoplasm and the nuclear envelopee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsGRXS17 resides in both the cytoplasm and the nuclear envelope”
t0“rice”
Claim · model-written summary
Under drought, plants with reduced OsGRXS17 had lower water loss and stomatal conductance than wild type.
- Taxon
- riceOryza sativae0
- Treatment
- reduced OsGRXS17 expressione0
- Gene
- OsGRXS17e0
- Outcome
- rates of water loss and stomatal conductancee0
- Direction
- lowere0
- Qualifier
- Under drought stress conditionse0
- Comparator
- wild-type controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought stress conditions, rice plants with reduced OsGRXS17 expression showed lower rates of water loss and stomatal conductance, higher relative water content, and enhanced survival compared to wild-type controls.”
Claim · model-written summary
Under drought, plants with reduced OsGRXS17 had higher relative water content than wild type.
- Taxon
- riceOryza sativae0
- Treatment
- reduced OsGRXS17 expressione0
- Gene
- OsGRXS17e0
- Outcome
- relative water contente0
- Direction
- highere0
- Comparator
- wild-type controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought stress conditions, rice plants with reduced OsGRXS17 expression showed lower rates of water loss and stomatal conductance, higher relative water content, and enhanced survival compared to wild-type controls.”
Claim · model-written summary
Under drought, plants with reduced OsGRXS17 had enhanced survival over wild type.
- Taxon
- riceOryza sativae0
- Treatment
- reduced OsGRXS17 expressione0
- Gene
- OsGRXS17e0
- Outcome
- survivale0
- Direction
- enhancede0
- Comparator
- wild-type controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Under drought stress conditions, rice plants with reduced OsGRXS17 expression showed lower rates of water loss and stomatal conductance, higher relative water content, and enhanced survival compared to wild-type controls.”
Claim · model-written summary
OsGRXS17 downregulation elevated guard cell hydrogen peroxide production.
- Taxon
- riceOryza sativat0
- Treatment
- OsGRXS17 down-regulatede0
- Gene
- OsGRXS17e0
- Outcome
- H 2 O 2 production within the guard cellse0
- Direction
- elevatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsGRXS17 down-regulated plants revealed an elevation in H 2 O 2 production within the guard cells”
t0“rice”
Claim · model-written summary
OsGRXS17 downregulation increased ABA sensitivity.
- Taxon
- riceOryza sativat0
- Treatment
- OsGRXS17 down-regulatede0
- Gene
- OsGRXS17e0
- Outcome
- sensitivity to ABAe0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsGRXS17 down-regulated plants revealed an elevation in H 2 O 2 production within the guard cells, increased sensitivity to ABA”
t0“rice”
Claim · model-written summary
OsGRXS17 downregulation reduced stomatal apertures.
- Taxon
- riceOryza sativat0
- Treatment
- OsGRXS17 down-regulatede0
- Gene
- OsGRXS17e0
- Outcome
- stomatal aperturese0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsGRXS17 down-regulated plants revealed an elevation in H 2 O 2 production within the guard cells, increased sensitivity to ABA, and a reduction in stomatal apertures”
t0“rice”
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See all 31 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.