Abstract
Natural disasters, including drought and salt stress, seriously threaten food security. In previous work we cloned a key zinc finger transcription factor gene, Drought and Salt Tolerance (DST), a negative regulator of drought and salt tolerance that controls stomatal aperture in rice. However, the exact mechanism by which DST regulates the expression of target genes remains unknown. In the present study, we demonstrated that DST Co-activator 1 (DCA1), a previously unknown CHY zinc finger protein, acts as an interacting co-activator of DST. DST was found to physically interact with itself and to form a heterologous tetramer with DCA1. This transcriptional complex appears to regulate the expression of peroxidase 24 precursor (Prx 24), a gene encoding an H2O2 scavenger that is more highly expressed in guard cells. Downregulation of DCA1 significantly enhanced drought and salt tolerance in rice, and overexpression of DCA1 increased sensitivity to stress treatment. These phenotypes were mainly influenced by DCA1 and negatively regulated stomatal closure through the direct modulation of genes associated with H2O2 homeostasis. Our findings establish a framework for plant drought and salt stress tolerance through the DCA1-DST-Prx24 pathway. Moreover, due to the evolutionary and functional conservation of DCA1 and DST in plants, engineering of this pathway has the potential to improve tolerance to abiotic stress in other important crop species.
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Claims 6
Claim · model-written summary
DCA1 acts as an interacting co-activator of DST.
- Taxon
- Rice
Oryza sativat0 - Gene
- DCA1e1
- Outcome
- interacting co-activator of DSTe1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“DST Co-activator 1 (DCA1), a previously unknown CHY zinc finger protein, acts as an interacting co-activator of DST”
t0“DCA1 Acts as a Transcriptional Co-activator of DST and Contributes to Drought and Salt Tolerance in Rice”
Claim · model-written summary
DST physically interacts with itself and forms a heterologous tetramer with DCA1.
- Taxon
- RiceOryza sativat0
- Gene
- DSTe2
- Outcome
- physically interact with itselfe2
- Qualifier
- to form a heterologous tetramer with DCA1e2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“DST was found to physically interact with itself and to form a heterologous tetramer with DCA1”
t0“DCA1 Acts as a Transcriptional Co-activator of DST and Contributes to Drought and Salt Tolerance in Rice”
Claim · model-written summary
The DCA1-DST complex appears to regulate expression of peroxidase 24 precursor (Prx 24), which is more highly expressed in guard cells.
- Taxon
- RiceOryza sativat0
- Organ / stage
- guard cellse3
- Gene
- peroxidase 24 precursor (Prx 24)e3
- Outcome
- expressione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“This transcriptional complex appears to regulate the expression of peroxidase 24 precursor (Prx 24), a gene encoding an H2O2 scavenger that is more highly expressed in guard cells”
t0“DCA1 Acts as a Transcriptional Co-activator of DST and Contributes to Drought and Salt Tolerance in Rice”
Claim · model-written summary
Downregulation of DCA1 significantly enhanced drought and salt tolerance in rice.
- Taxon
- riceOryza sativae4
- Treatment
- Downregulation of DCA1e4
- Gene
- DCA1e4
- Outcome
- drought and salt tolerancee4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Downregulation of DCA1 significantly enhanced drought and salt tolerance in rice”
Claim · model-written summary
Overexpression of DCA1 increased sensitivity to stress treatment in rice.
- Taxon
- RiceOryza sativat0
- Treatment
- overexpression of DCA1e5
- Gene
- DCA1e5
- Outcome
- sensitivity to stress treatmente5
- Direction
- increasede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e5“overexpression of DCA1 increased sensitivity to stress treatment”
t0“DCA1 Acts as a Transcriptional Co-activator of DST and Contributes to Drought and Salt Tolerance in Rice”
Claim · model-written summary
DCA1 negatively regulates stomatal closure through direct modulation of H2O2 homeostasis genes.
- Taxon
- RiceOryza sativat0
- Gene
- DCA1e6
- Outcome
- stomatal closuree6
- Qualifier
- through the direct modulation of genes associated with H2O2 homeostasise6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“These phenotypes were mainly influenced by DCA1 and negatively regulated stomatal closure through the direct modulation of genes associated with H2O2 homeostasis”
t0“DCA1 Acts as a Transcriptional Co-activator of DST and Contributes to Drought and Salt Tolerance in Rice”
The same questions in other papers
loss of function · tolerance: abiotic · Oryza sativa (rice)Positive and negative results
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