Abstract
Summary Cellular abscisic acid (ABA) concentration is determined by both de novo biosynthesis and recycling via β‐glucosidase(s). However, which rice β‐glucosidase(s) are involved in this process remains unknown. Here, we report on a chloroplastic β‐glucosidase isoenzyme, Os3BGlu6, that functions in ABA recycling in rice. Disruption of Os3BGlu6 in rice resulted in dwarfism, lower ABA content in leaves, drought‐sensitivity, lower photosynthesis rate and higher intercellular CO 2 concentration. Os3BGlu6 could hydrolyze ABA‐GE to ABA in vitro . The reversion and overexpression rice lines restored or increased the drought tolerance as shown by the higher β‐glucosidase activity, ABA concentrations and expressions of ABA‐ and drought‐responsive genes. Drought induced Os3BGlu6 to form dimers, and the degree of polymerization correlated well with the increase in cellular ABA concentrations and drought tolerance in rice. Os3BGlu6 was responsive to drought and ABA treatments, and the protein was localized to the chloroplast. Disruption of Os3BGlu6 resulted in the increased stomatal density and impaired stomatal movement. Transcriptomics revealed that disruption of Os3BGlu6 resulted in chloroplastic oxidative stress and lowered Rubisco activity even under normal conditions. Taken together, these results suggest that chloroplastically localized Os3BGlu6 significantly affects cellular ABA pools, thereby affecting drought tolerance and photosynthesis in rice.
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Claims 18
Claim · model-written summary
Disruption of Os3BGlu6 caused dwarfism.
- Taxon
- rice
Oryza sativat0 - Treatment
- Disruption of Os3BGlu6e0
- Gene
- Os3BGlu6t0
- Outcome
- dwarfisme0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“Disruption of Os3BGlu6 in rice resulted in dwarfism, lower ABA content in leaves, drought‐sensitivity, lower photosynthesis rate and higher intercellular CO 2 concentration.”
Claim · model-written summary
Disruption of Os3BGlu6 caused ABA content in leaves.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of Os3BGlu6e0
- Gene
- Os3BGlu6t0
- Outcome
- ABA content in leavese0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“Disruption of Os3BGlu6 in rice resulted in dwarfism, lower ABA content in leaves, drought‐sensitivity, lower photosynthesis rate and higher intercellular CO 2 concentration.”
Claim · model-written summary
Disruption of Os3BGlu6 caused drought‐sensitivity.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of Os3BGlu6e0
- Gene
- Os3BGlu6t0
- Outcome
- drought‐sensitivitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“Disruption of Os3BGlu6 in rice resulted in dwarfism, lower ABA content in leaves, drought‐sensitivity, lower photosynthesis rate and higher intercellular CO 2 concentration.”
Claim · model-written summary
Disruption of Os3BGlu6 caused photosynthesis rate.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of Os3BGlu6e0
- Gene
- Os3BGlu6t0
- Outcome
- photosynthesis ratee0
- Direction
- lowere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“Disruption of Os3BGlu6 in rice resulted in dwarfism, lower ABA content in leaves, drought‐sensitivity, lower photosynthesis rate and higher intercellular CO 2 concentration.”
Claim · model-written summary
Disruption of Os3BGlu6 caused intercellular CO 2 concentration.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of Os3BGlu6e0
- Gene
- Os3BGlu6t0
- Outcome
- intercellular CO 2 concentratione0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“Disruption of Os3BGlu6 in rice resulted in dwarfism, lower ABA content in leaves, drought‐sensitivity, lower photosynthesis rate and higher intercellular CO 2 concentration.”
Claim · model-written summary
Os3BGlu6 hydrolyzed ABA‐GE to ABA in vitro.
- Taxon
- riceOryza sativat0
- Gene
- Os3BGlu6t0
- Compound
- ABA‐GEe0
- Outcome
- ABA‐GE to ABAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“Os3BGlu6 could hydrolyze ABA‐GE to ABA in vitro”
Claim · model-written summary
Reversion and overexpression lines improved drought tolerance.
- Taxon
- riceOryza sativat0
- Material
- reversion and overexpression rice linese0
- Gene
- Os3BGlu6t0
- Outcome
- drought tolerancee0
- Direction
- restored or increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“The reversion and overexpression rice lines restored or increased the drought tolerance as shown by the higher β‐glucosidase activity, ABA concentrations and expressions of ABA‐ and drought‐responsive genes.”
Claim · model-written summary
Reversion and overexpression lines improved β‐glucosidase activity.
- Taxon
- riceOryza sativat0
- Material
- reversion and overexpression rice linese0
- Gene
- Os3BGlu6t0
- Outcome
- β‐glucosidase activitye0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“The reversion and overexpression rice lines restored or increased the drought tolerance as shown by the higher β‐glucosidase activity, ABA concentrations and expressions of ABA‐ and drought‐responsive genes.”
Claim · model-written summary
Reversion and overexpression lines improved ABA concentrations.
- Taxon
- riceOryza sativat0
- Material
- reversion and overexpression rice linese0
- Gene
- Os3BGlu6t0
- Outcome
- ABA concentrationse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“The reversion and overexpression rice lines restored or increased the drought tolerance as shown by the higher β‐glucosidase activity, ABA concentrations and expressions of ABA‐ and drought‐responsive genes.”
Claim · model-written summary
Reversion and overexpression lines improved expressions of ABA‐ and drought‐responsive genes.
- Taxon
- riceOryza sativat0
- Material
- reversion and overexpression rice linese0
- Gene
- Os3BGlu6t0
- Outcome
- expressions of ABA‐ and drought‐responsive genese0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“The reversion and overexpression rice lines restored or increased the drought tolerance as shown by the higher β‐glucosidase activity, ABA concentrations and expressions of ABA‐ and drought‐responsive genes.”
Claim · model-written summary
Drought induced Os3BGlu6 dimers.
- Taxon
- riceOryza sativat0
- Treatment
- Droughte0
- Gene
- Os3BGlu6t0
- Outcome
- Os3BGlu6 to form dimerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“Drought induced Os3BGlu6 to form dimers”
Claim · model-written summary
Os3BGlu6 polymerization correlated with increased ABA concentrations and drought tolerance.
- Taxon
- riceOryza sativat0
- Gene
- Os3BGlu6t0
- Outcome
- increase in cellular ABA concentrations and drought tolerancee0
- Direction
- increasee0
- Qualifier
- degree of polymerizatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“degree of polymerization correlated well with the increase in cellular ABA concentrations and drought tolerance in rice”
Claim · model-written summary
Os3BGlu6 responded to drought and ABA treatments.
- Taxon
- riceOryza sativat0
- Treatment
- drought and ABA treatmentse0
- Gene
- Os3BGlu6t0
- Outcome
- Os3BGlu6 was responsivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“Os3BGlu6 was responsive to drought and ABA treatments”
Claim · model-written summary
Os3BGlu6 protein localized to chloroplasts.
- Taxon
- riceOryza sativat0
- Gene
- Os3BGlu6t0
- Outcome
- localized to the chloroplaste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“protein was localized to the chloroplast”
Claim · model-written summary
Os3BGlu6 disruption increased stomatal density.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of Os3BGlu6e0
- Gene
- Os3BGlu6t0
- Outcome
- stomatal densitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“Disruption of Os3BGlu6 resulted in the increased stomatal density and impaired stomatal movement”
Claim · model-written summary
Os3BGlu6 disruption impaired stomatal movement.
- Taxon
- riceOryza sativat0
- Treatment
- Disruption of Os3BGlu6e0
- Gene
- Os3BGlu6t0
- Outcome
- stomatal movemente0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“Disruption of Os3BGlu6 resulted in the increased stomatal density and impaired stomatal movement”
Claim · model-written summary
Os3BGlu6 disruption caused chloroplast oxidative stress.
- Taxon
- riceOryza sativat0
- Treatment
- disruption of Os3BGlu6e0
- Gene
- Os3BGlu6t0
- Outcome
- chloroplastic oxidative stresse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“disruption of Os3BGlu6 resulted in chloroplastic oxidative stress and lowered Rubisco activity even under normal conditions”
Claim · model-written summary
Os3BGlu6 disruption lowered Rubisco activity.
- Taxon
- riceOryza sativat0
- Treatment
- disruption of Os3BGlu6e0
- Gene
- Os3BGlu6t0
- Outcome
- Rubisco activitye0
- Direction
- lowerede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Os3BGlu6 contributes significantly to cellular ABA pools and impacts drought tolerance and photosynthesis in rice”
e0“disruption of Os3BGlu6 resulted in chloroplastic oxidative stress and lowered Rubisco activity even under normal conditions”
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