Abstract
MicroRNAs function in a range of developmental processes. Here, we demonstrate that miR847 targets the mRNA of the auxin/indole acetic acid (Aux/IAA) repressor-encoding gene IAA28 for cleavage. The rapidly increased accumulation of miR847 in Arabidopsis thaliana coincided with reduced IAA28 mRNA levels upon auxin treatment. This induction of miR847 by auxin was abolished in auxin receptor tir1-1 and auxin-resistant axr1-3 mutants. Further analysis demonstrates that miR847 functions as a positive regulator of auxin-mediated lateral organ development by cleaving IAA28 mRNA. Importantly, the ectopic expression of miR847 increases the expression of cell cycle genes as well as the neoplastic activity of leaf cells, prolonging later-stage rosette leaf growth and producing leaves with serrated margins. Moreover, both miR847 and IAA28 mRNAs are specifically expressed in marginal meristems of rosette leaves and lateral root initiation sites. Our data indicate that auxin-dependent induction of miR847 positively regulates meristematic competence by clearing IAA28 mRNA to upregulate auxin signaling, thereby determining the duration of cell proliferation and lateral organ growth in Arabidopsis. IAA28 mRNA encodes an Aux/IAA repressor protein, which is degraded through the proteasome in response to auxin. Altered signal sensitization to IAA28 mRNA levels, together with targeted IAA28 degradation, ensures a robust signal derepression.
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Claims 12
Claim · model-written summary
miR847 cleaved IAA28 mRNA.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- IAA28e0
- Outcome
- cleavagee0
- Qualifier
- miR847 targets the mRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“miR847 targets the mRNA of the auxin/indole acetic acid (Aux/IAA) repressor-encoding gene IAA28 for cleavage”
Claim · model-written summary
Auxin treatment increased miR847 accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- auxin treatmente0
- Gene
- miR847e0
- Outcome
- accumulation of miR847e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“rapidly increased accumulation of miR847 in Arabidopsis thaliana coincided with reduced IAA28 mRNA levels upon auxin treatment”
Claim · model-written summary
IAA28 mRNA levels fell after auxin treatment.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- auxin treatmente0
- Gene
- IAA28e0
- Outcome
- IAA28 mRNA levelse0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“reduced IAA28 mRNA levels upon auxin treatment”
Claim · model-written summary
Auxin induction of miR847 was abolished in tir1-1 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- tir1-1e0
- Gene
- miR847e0
- Outcome
- induction of miR847 by auxin was abolishede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“induction of miR847 by auxin was abolished in auxin receptor tir1-1 and auxin-resistant axr1-3 mutants”
Claim · model-written summary
Auxin induction of miR847 was abolished in axr1-3 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- axr1-3 mutantse0
- Gene
- miR847e0
- Outcome
- induction of miR847 by auxin was abolishede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“induction of miR847 by auxin was abolished in auxin receptor tir1-1 and auxin-resistant axr1-3 mutants”
Claim · model-written summary
miR847 positively regulated auxin-mediated lateral organ development by cleaving IAA28 mRNA.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- miR847e0
- Outcome
- auxin-mediated lateral organ developmente0
- Qualifier
- by cleaving IAA28 mRNAe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“miR847 functions as a positive regulator of auxin-mediated lateral organ development by cleaving IAA28 mRNA”
Claim · model-written summary
Ectopic miR847 expression increased cell cycle gene expression.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ectopic expression of miR847e0
- Gene
- miR847e0
- Outcome
- expression of cell cycle genese0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“ectopic expression of miR847 increases the expression of cell cycle genes”
Claim · model-written summary
Ectopic miR847 expression increased neoplastic activity of leaf cells.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- leaf cellse0
- Treatment
- ectopic expression of miR847e0
- Gene
- miR847e0
- Outcome
- neoplastic activity of leaf cellse0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“ectopic expression of miR847 increases the expression of cell cycle genes as well as the neoplastic activity of leaf cells”
Claim · model-written summary
Ectopic miR847 expression prolonged later rosette leaf growth.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- rosette leafe0
- Treatment
- ectopic expression of miR847e0
- Gene
- miR847e0
- Outcome
- later-stage rosette leaf growthe0
- Direction
- prolonginge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“ectopic expression of miR847 increases the expression of cell cycle genes as well as the neoplastic activity of leaf cells, prolonging later-stage rosette leaf growth”
Claim · model-written summary
Ectopic miR847 expression produced leaves with serrated margins.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ectopic expression of miR847e0
- Gene
- miR847e0
- Outcome
- leaves with serrated marginse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“ectopic expression of miR847 increases the expression of cell cycle genes as well as the neoplastic activity of leaf cells, prolonging later-stage rosette leaf growth and producing leaves with serrated margins”
Claim · model-written summary
miR847 and IAA28 mRNAs were expressed in rosette leaf marginal meristems and lateral root initiation sites.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- marginal meristems of rosette leaves and lateral root initiation sitese0
- Gene
- miR847 and IAA28 mRNAse0
- Outcome
- specifically expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“both miR847 and IAA28 mRNAs are specifically expressed in marginal meristems of rosette leaves and lateral root initiation sites”
Claim · model-written summary
Auxin-induced miR847 was interpreted to clear IAA28 mRNA and increase auxin signaling and meristematic competence.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- miR847e0
- Outcome
- meristematic competencee0
- Qualifier
- by clearing IAA28 mRNA to upregulate auxin signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis”
e0“auxin-dependent induction of miR847 positively regulates meristematic competence by clearing IAA28 mRNA to upregulate auxin signaling”
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See all 109 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.