Abstract
Glycolysis is a ubiquitous pathway thought to be essential for the production of oil in developing seeds of Arabidopsis thaliana and oil crops. Compartmentation of primary metabolism in developing embryos poses a significant challenge for testing this hypothesis and for the engineering of seed biomass production. It also raises the question whether there is a preferred route of carbon from imported photosynthate to seed oil in the embryo. Plastidic pyruvate kinase catalyzes a highly regulated, ATP-producing reaction of glycolysis. The Arabidopsis genome encodes 14 putative isoforms of pyruvate kinases. Three genes encode subunits alpha, beta(1), and beta(2) of plastidic pyruvate kinase. The plastid enzyme prevalent in developing seeds likely has a subunit composition of 4alpha4beta(1), is most active at pH 8.0, and is inhibited by Glu. Disruption of the gene encoding the beta(1) subunit causes a reduction in plastidic pyruvate kinase activity and 60% reduction in seed oil content. The seed oil phenotype is fully restored by expression of the beta(1) subunit-encoding cDNA and partially by the beta(2) subunit-encoding cDNA. Therefore, the identified pyruvate kinase catalyzes a crucial step in the conversion of photosynthate into oil, suggesting a preferred plastid route from its substrate phosphoenolpyruvate to fatty acids.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Disrupting the beta(1) subunit gene reduced plastidic pyruvate kinase activity.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Treatment
- Disruption of the gene encoding the beta(1) subunite1
- Gene
- beta(1) subunite1
- Outcome
- plastidic pyruvate kinase activitye1
- Direction
- reductione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing seeds of Arabidopsis thaliana”
e1“Disruption of the gene encoding the beta(1) subunit causes a reduction in plastidic pyruvate kinase activity”
Claim · model-written summary
Disrupting the beta(1) subunit gene reduced seed oil content by 60%.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Disruption of the gene encoding the beta(1) subunite1
- Gene
- beta(1) subunite1
- Outcome
- seed oil contente2
- Direction
- reductione2
- Value
- 60%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing seeds of Arabidopsis thaliana”
e1“Disruption of the gene encoding the beta(1) subunit causes a reduction in plastidic pyruvate kinase activity”
e2“60% reduction in seed oil content”
Claim · model-written summary
Expression of the beta(1) subunit-encoding cDNA fully restored the seed oil phenotype.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- expression of the beta(1) subunit-encoding cDNAe1
- Gene
- beta(1) subunite1
- Outcome
- seed oil phenotypee1
- Direction
- restorede1
- Qualifier
- fullye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing seeds of Arabidopsis thaliana”
e1“The seed oil phenotype is fully restored by expression of the beta(1) subunit-encoding cDNA and partially by the beta(2) subunit-encoding cDNA”
Claim · model-written summary
Expression of the beta(2) subunit-encoding cDNA partially restored the seed oil phenotype.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- beta(2) subunit-encoding cDNAe1
- Gene
- beta(2) subunite1
- Outcome
- seed oil phenotypee1
- Direction
- restorede1
- Qualifier
- partiallye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing seeds of Arabidopsis thaliana”
e1“The seed oil phenotype is fully restored by expression of the beta(1) subunit-encoding cDNA and partially by the beta(2) subunit-encoding cDNA”
Claim · model-written summary
The plastidic pyruvate kinase in developing seeds likely has a 4alpha4beta(1) subunit composition.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Organ / stage
- developing seedse1
- Outcome
- subunit compositione1
- Value
- 4alpha4beta(1)e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing seeds of Arabidopsis thaliana”
e1“The plastid enzyme prevalent in developing seeds likely has a subunit composition of 4alpha4beta(1)”
Claim · model-written summary
The plastidic pyruvate kinase is most active at pH 8.0.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Outcome
- most activee1
- Value
- pH 8.0e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing seeds of Arabidopsis thaliana”
e1“is most active at pH 8.0”
Claim · model-written summary
The plastidic pyruvate kinase is inhibited by Glu.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Compound
- Glue1
- Outcome
- is inhibited by Glue1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing seeds of Arabidopsis thaliana”
e1“is inhibited by Glu”
Claim · model-written summary
Three genes encode the alpha, beta(1) and beta(2) subunits of plastidic pyruvate kinase.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- alpha, beta(1), and beta(2)e1
- Outcome
- Three genes encode subunitse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing seeds of Arabidopsis thaliana”
e1“Three genes encode subunits alpha, beta(1), and beta(2) of plastidic pyruvate kinase”
Claim · model-written summary
The pyruvate kinase suggests a preferred plastid route from phosphoenolpyruvate to fatty acids.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- pyruvate kinasee1
- Outcome
- preferred plastid routee1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing seeds of Arabidopsis thaliana”
e1“the identified pyruvate kinase catalyzes a crucial step in the conversion of photosynthate into oil, suggesting a preferred plastid route from its substrate phosphoenolpyruvate to fatty acids”
Claim · model-written summary
The identified pyruvate kinase catalyzes a crucial step in the conversion of photosynthate into oil.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- pyruvate kinasee1
- Outcome
- conversion of photosynthate into oile1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“developing seeds of Arabidopsis thaliana”
e1“the identified pyruvate kinase catalyzes a crucial step in the conversion of photosynthate into oil, suggesting a preferred plastid route from its substrate phosphoenolpyruvate to fatty acids”
The same questions in other papers
loss of function · quality: oil · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.