Abstract
Seed oils are important natural resources used in the processing and preparation of food. Histone modifications represent key epigenetic mechanisms that regulate gene expression, plant growth and development. However, histone modification events during fatty acid (FA) biosynthesis are not well understood. Here, we demonstrate that a mutation of the histone acetyltransferase GCN5 can decrease the ratio of α-linolenic acid (ALA) to linoleic acid (LA) in seed oil. Using RNA-Seq and ChIP assays, we identified FAD3, LACS2, LPP3 and PLAIIIβ as the targets of GCN5. Notably, the GCN5-dependent H3K9/14 acetylation of FAD3 determined the expression levels of FAD3 in Arabidopsis thaliana seeds, and the ratio of ALA/LA in the gcn5 mutant was rescued to the wild-type levels through the overexpression of FAD3. The results of this study indicated that GCN5 modulated FA biosynthesis by affecting the acetylation levels of FAD3. We provide evidence that histone acetylation is involved in FA biosynthesis in Arabidopsis seeds and might contribute to the optimization of the nutritional structure of edible oils through epigenetic engineering.
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Claims 5
Claim · model-written summary
GCN5 mutation decreases the seed oil ALA to LA ratio.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- mutation of the histone acetyltransferase GCN5e0
- Gene
- GCN5e0
- Outcome
- ratio of α-linolenic acid (ALA) to linoleic acid (LA) in seed oile0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“a mutation of the histone acetyltransferase GCN5 can decrease the ratio of α-linolenic acid (ALA) to linoleic acid (LA) in seed oil”
Claim · model-written summary
FAD3, LACS2, LPP3 and PLAIIIβ were identified as GCN5 targets.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- GCN5e0
- Outcome
- FAD3, LACS2, LPP3 and PLAIIIβ as the targets of GCN5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“we identified FAD3, LACS2, LPP3 and PLAIIIβ as the targets of GCN5”
Claim · model-written summary
GCN5-dependent H3K9/14 acetylation of FAD3 determined FAD3 expression in seeds.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- FAD3e0
- Outcome
- expression levels of FAD3e0
- Qualifier
- GCN5-dependent H3K9/14 acetylation of FAD3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“the GCN5-dependent H3K9/14 acetylation of FAD3 determined the expression levels of FAD3 in Arabidopsis thaliana seeds”
Claim · model-written summary
FAD3 overexpression restored the mutant ALA/LA ratio to wild-type levels.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Material
- gcn5 mutante0
- Treatment
- overexpression of FAD3e0
- Gene
- FAD3e0
- Outcome
- ratio of ALA/LAe0
- Direction
- rescuede0
- Comparator
- wild-type levelse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“the ratio of ALA/LA in the gcn5 mutant was rescued to the wild-type levels through the overexpression of FAD3”
Claim · model-written summary
GCN5 modulates fatty acid biosynthesis through FAD3 acetylation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Gene
- GCN5e0
- Outcome
- FA biosynthesise0
- Qualifier
- affecting the acetylation levels of FAD3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Arabidopsis thaliana”
e0“GCN5 modulated FA biosynthesis by affecting the acetylation levels of FAD3”
The same questions in other papers
loss of function · quality: oil · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- positive
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.