Abstract
Brassinosteroids (BRs) are a class of plant-specific steroidal hormones that play important roles in multiple biological processes. In this paper, a classic rice mutant gsor300084, showing erect leaves and semi-dwarf stature, was characterized. Morphological analysis in darkness showed that the mesocotyl of the gsor300084 mutant did not elongate when grown in darkness. Coleoptile elongation and root growth were less affected by the exogenous application of brassinolide (BL), the most active form of BR, in gsor300084 than in the wild-type rice variety Matsumae. Lamina joint bending analysis also showed that gsor300084 was less sensitive to exogenous BL than Matsumae. These results suggested that the gsor300084 mutant is defective in BR sensitivity. Map-based cloning indicated that gsor300084 is a novel allelic mutant of the DWARF61 (D61) gene, which encodes the putative BR receptor OsBRI1. A single-base mutation appears in the LRR domain of OsBRI1, changing the 444th amino acid from tryptophan (W) to arginine (R). Subcellular localization analysis suggested that both the wild-type and mutant OsBRI1 protein are localized at the cytoplasmic membrane. Structure modeling revealed that the W444R substitution may affect the perception of BRs by the LRR domain.
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Claims 10
Claim · model-written summary
The gsor300084 rice mutant had erect leaves and a semi-dwarf stature.
- Taxon
- rice
Oryza sativae0 - Material
- gsor300084e0
- Outcome
- erect leaves and semi-dwarf staturee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a classic rice mutant gsor300084, showing erect leaves and semi-dwarf stature”
Claim · model-written summary
The gsor300084 mesocotyl did not elongate in darkness.
- Taxon
- riceOryza sativat0
- Material
- gsor300084e0
- Organ / stage
- mesocotyle0
- Treatment
- darknesse0
- Outcome
- mesocotyl of the gsor300084 mutant did not elongatee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the mesocotyl of the gsor300084 mutant did not elongate when grown in darkness”
t0“rice”
Claim · model-written summary
With exogenous brassinolide, gsor300084 coleoptile elongation responded less than Matsumae.
- Taxon
- riceOryza sativae0
- Material
- gsor300084e0
- Treatment
- exogenous application of brassinolide (BL)e0
- Outcome
- Coleoptile elongatione0
- Direction
- less affectede0
- Comparator
- Matsumaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coleoptile elongation and root growth were less affected by the exogenous application of brassinolide (BL), the most active form of BR, in gsor300084 than in the wild-type rice variety Matsumae.”
Claim · model-written summary
With exogenous brassinolide, gsor300084 root growth responded less than Matsumae.
- Taxon
- riceOryza sativae0
- Material
- gsor300084e0
- Treatment
- exogenous application of brassinolide (BL)e0
- Outcome
- root growthe0
- Direction
- less affectede0
- Comparator
- Matsumaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Coleoptile elongation and root growth were less affected by the exogenous application of brassinolide (BL), the most active form of BR, in gsor300084 than in the wild-type rice variety Matsumae.”
Claim · model-written summary
gsor300084 lamina joint bending was less sensitive to exogenous brassinolide than Matsumae.
- Taxon
- riceOryza sativat0
- Material
- gsor300084e0
- Treatment
- exogenous BLe0
- Outcome
- Lamina joint bendinge0
- Direction
- less sensitivee0
- Comparator
- Matsumaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Lamina joint bending analysis also showed that gsor300084 was less sensitive to exogenous BL than Matsumae.”
t0“rice”
Claim · model-written summary
The authors inferred that gsor300084 has defective brassinosteroid sensitivity.
- Taxon
- riceOryza sativat0
- Material
- gsor300084e0
- Outcome
- BR sensitivitye0
- Direction
- defectivee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the gsor300084 mutant is defective in BR sensitivity”
t0“rice”
Claim · model-written summary
Map-based cloning identified gsor300084 as a novel D61 allelic mutant.
- Taxon
- riceOryza sativat0
- Material
- gsor300084e0
- Gene
- DWARF61 (D61)e0
- Outcome
- novel allelic mutante0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“gsor300084 is a novel allelic mutant of the DWARF61 (D61) gene, which encodes the putative BR receptor OsBRI1.”
t0“rice”
Claim · model-written summary
A single-base OsBRI1 LRR-domain mutation changed amino acid 444 from tryptophan to arginine.
- Taxon
- riceOryza sativat0
- Gene
- OsBRI1e0
- Outcome
- changing the 444th amino acid from tryptophan (W) to arginine (R)e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A single-base mutation appears in the LRR domain of OsBRI1, changing the 444th amino acid from tryptophan (W) to arginine (R).”
t0“rice”
Claim · model-written summary
Wild-type and mutant OsBRI1 localized at the cytoplasmic membrane.
- Taxon
- riceOryza sativat0
- Gene
- OsBRI1e0
- Outcome
- localized at the cytoplasmic membranee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both the wild-type and mutant OsBRI1 protein are localized at the cytoplasmic membrane”
t0“rice”
Claim · model-written summary
Structure modeling suggested that W444R may affect brassinosteroid perception by the LRR domain.
- Taxon
- riceOryza sativat0
- Gene
- W444Re0
- Outcome
- perception of BRse0
- Qualifier
- by the LRR domaine0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the W444R substitution may affect the perception of BRs by the LRR domain”
t0“rice”
The same questions in other papers
brassinolide · growth · Oryza sativa (rice)Results differ
4 papers in the same direction as this paper · 27 in the opposite direction
- positive
- positive
- positive
28 papers more in this group
See all 32 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.