Abstract
Lateral roots of intact summer squash seedlings (Cucurbita pepo L.) were used to quantify the effects of boron deficiency on DNA synthesis, protein synthesis, and respiration. The temporal relationship between changes in these metabolic activities and the cessation of root elongation caused by boron deprivation was determined. Transferring 5-day-old squash seedlings to a hydroponic culture medium without boron for 6 hours resulted in a 62% reduction in net root elongation and a 30% decrease in the incorporation of [(3)H]thymidine into DNA by root tips (apical 5-millimeter segments). At this time, root tips from both boron-deficient and boron-sufficient plants exhibited nearly identical rates of incorporation of [(14)C]leucine into protein and respiration as measured by O(2) consumption. After an additional 6 hours of boron deprivation, root elongation had nearly ceased. Concomitantly, DNA synthesis in root apices was 66% less than in the boron-sufficient control plants and protein synthesis was reduced 43%. O(2) consumption remained the same for both treatments. The decline and eventual cessation of root elongation correlated temporally with the decrease in DNA synthesis, but preceded changes in protein synthesis and respiration. These results suggest that boron is required for continued DNA synthesis and cell division in root meristems.
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Claims 10
Claim · model-written summary
Six hours without boron reduced squash root elongation by 62%.
Not reportedComparator
Verbatim
e0“Transferring 5-day-old squash seedlings to a hydroponic culture medium without boron for 6 hours resulted in a 62% reduction in net root elongation”
Claim · model-written summary
Six hours without boron reduced thymidine incorporation into root tip DNA by 30%.
- Taxon
- Cucurbita pepo
Cucurbita pepot0 - Outcome
- incorporation of [(3)H]thymidine into DNAe0
- Direction
- decreasee0
- Value
- 30%e0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a 30% decrease in the incorporation of [(3)H]thymidine into DNA by root tips (apical 5-millimeter segments)”
t0“Metabolic Requirement of Cucurbita pepo for Boron”
Claim · model-written summary
At six hours protein synthesis rates were nearly identical in deficient and sufficient plants.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Treatment
- boron-deficiente0
- Outcome
- rates of incorporation of [(14)C]leucine into proteine0
- Direction
- nearly identicale0
- Comparator
- boron-sufficiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root tips from both boron-deficient and boron-sufficient plants exhibited nearly identical rates of incorporation of [(14)C]leucine into protein”
t0“Cucurbita pepo”
Claim · model-written summary
At six hours respiration rates were nearly identical in deficient and sufficient plants.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Treatment
- boron-deficiente0
- Outcome
- respiration as measured by O(2) consumptione0
- Direction
- nearly identicale0
- Comparator
- boron-sufficiente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“root tips from both boron-deficient and boron-sufficient plants exhibited nearly identical rates of incorporation of [(14)C]leucine into protein and respiration as measured by O(2) consumption”
t0“Cucurbita pepo”
Claim · model-written summary
After twelve hours without boron, root elongation had nearly ceased.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Treatment
- boron deprivatione0
- Outcome
- root elongatione0
- Direction
- nearly ceasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After an additional 6 hours of boron deprivation, root elongation had nearly ceased”
t0“Cucurbita pepo”
Claim · model-written summary
After twelve hours DNA synthesis was 66% less than in sufficient controls.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Treatment
- Boront0
- Outcome
- DNA synthesis in root apicese0
- Direction
- lesse0
- Value
- 66%e0
- Comparator
- boron-sufficient control plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA synthesis in root apices was 66% less than in the boron-sufficient control plants”
t0“Cucurbita pepo for Boron”
Claim · model-written summary
After twelve hours protein synthesis was reduced 43%.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Treatment
- Boront0
- Outcome
- protein synthesise0
- Direction
- reducede0
- Value
- 43%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“protein synthesis was reduced 43%”
t0“Cucurbita pepo for Boron”
Claim · model-written summary
Oxygen consumption remained the same under both boron treatments.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Treatment
- Boront0
- Outcome
- O(2) consumptione0
- Direction
- remained the samee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“O(2) consumption remained the same for both treatments”
t0“Cucurbita pepo for Boron”
Claim · model-written summary
Root elongation decline coincided with DNA synthesis decline and preceded protein synthesis and respiration changes.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Organ / stage
- roote0
- Outcome
- decline and eventual cessation of root elongatione0
- Qualifier
- decrease in DNA synthesise0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The decline and eventual cessation of root elongation correlated temporally with the decrease in DNA synthesis, but preceded changes in protein synthesis and respiration”
t0“Cucurbita pepo”
Claim · model-written summary
Boron may be required for continued DNA synthesis and cell division in root meristems.
- Taxon
- Cucurbita pepoCucurbita pepot0
- Organ / stage
- root meristemse0
- Outcome
- continued DNA synthesis and cell divisione0
- Qualifier
- boron is requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These results suggest that boron is required for continued DNA synthesis and cell division in root meristems”
t0“Cucurbita pepo”
The same questions in other papers
boron atom · growth · Cucurbita (pumpkins)Results agree
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This paper is about lacking the nutrient, so it counts the other way round here.
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.