Paper · 2001 · PLANT PHYSIOLOGY

Formation of Rhamnogalacturonan II-Borate Dimer in Pectin Determines Cell Wall Thickness of Pumpkin Tissue

Tadashi Ishii; Toshiro Matsunaga; Noriko Hayashi

8
claims
16
verbatim quotes
187
citations
2
research areas
✓
Open access

Abstract

Boron (B) deficiency results in inhibition of pumpkin (Cucurbia moschata Duchesne) growth that is accompanied by swelling of the cell walls. Monomeric rhamnogalacturonan II (mRG-II) accounted for 80% to 90% of the total RG-II in B-deficient walls, whereas the borate ester cross-linked RG-II dimer (dRG-II-B) accounted for more than 80% of the RG-II in control plants. The results of glycosyl residue and glycosyl linkage composition analyses of the RG-II from control and B-deficient plants were similar. Thus, B deficiency does not alter the primary structure of RG-II. The addition of (10)B-enriched boric acid to B-deficient plants resulted within 5 h in the conversion of mRG-II to dRG-II-(10)B. The wall thickness of the (10)B-treated plants and control plants was similar. The formation and possible functions of a borate ester cross-linked RG-II in the cell walls are discussed.

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Claims 8

Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Boron deficiency inhibited pumpkin growth.

Taxon
Pumpkint0
Treatment
Boron (B) deficiencye1
Outcome
growthe1
Direction
inhibitione1

Not reportedComparator

Verbatim

t0

“Formation of Rhamnogalacturonan II-Borate Dimer in Pectin Determines Cell Wall Thickness of Pumpkin Tissue”

✓ located verbatim in the source · title, characters 0–106 · show in text ↑
e1

“Boron (B) deficiency results in inhibition of pumpkin (Cucurbia moschata Duchesne) growth that is accompanied by swelling of the cell walls”

✓ located verbatim in the source · abstract, characters 0–139 · show in text ↑
Observationobservedoriginal resultnegativemeasured

Claim · model-written summary

Boron deficiency was accompanied by swelling of the cell walls in pumpkin.

Taxon
Pumpkint0
Organ / stage
cell wallse1
Treatment
Boron (B) deficiencye1
Outcome
swelling of the cell wallse1

Verbatim

t0

“Formation of Rhamnogalacturonan II-Borate Dimer in Pectin Determines Cell Wall Thickness of Pumpkin Tissue”

✓ located verbatim in the source · title, characters 0–106 · show in text ↑
e1

“Boron (B) deficiency results in inhibition of pumpkin (Cucurbia moschata Duchesne) growth that is accompanied by swelling of the cell walls”

✓ located verbatim in the source · abstract, characters 0–139 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Monomeric RG-II (mRG-II) accounted for 80% to 90% of the total RG-II in boron-deficient pumpkin cell walls.

Taxon
Pumpkint0
Treatment
B-deficiente2
Outcome
Monomeric rhamnogalacturonan II (mRG-II)e2
Value
80% to 90%e2

Verbatim

t0

“Formation of Rhamnogalacturonan II-Borate Dimer in Pectin Determines Cell Wall Thickness of Pumpkin Tissue”

✓ located verbatim in the source · title, characters 0–106 · show in text ↑
e2

“Monomeric rhamnogalacturonan II (mRG-II) accounted for 80% to 90% of the total RG-II in B-deficient walls”

✓ located verbatim in the source · abstract, characters 141–246 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

The borate ester cross-linked RG-II dimer (dRG-II-B) accounted for more than 80% of the RG-II in control pumpkin plants.

Taxon
Pumpkint0
Outcome
borate ester cross-linked RG-II dimer (dRG-II-B)e3
Value
more than 80%e3
Qualifier
in control plantse3

Verbatim

t0

“Formation of Rhamnogalacturonan II-Borate Dimer in Pectin Determines Cell Wall Thickness of Pumpkin Tissue”

✓ located verbatim in the source · title, characters 0–106 · show in text ↑
e3

“the borate ester cross-linked RG-II dimer (dRG-II-B) accounted for more than 80% of the RG-II in control plants”

✓ located verbatim in the source · abstract, characters 256–367 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Glycosyl residue and glycosyl linkage composition of RG-II were similar between control and boron-deficient pumpkin plants.

Taxon
Pumpkint0
Treatment
B-deficient plantse4
Outcome
glycosyl residue and glycosyl linkage compositione4
Direction
similare4
Comparator
controle4

Verbatim

t0

“Formation of Rhamnogalacturonan II-Borate Dimer in Pectin Determines Cell Wall Thickness of Pumpkin Tissue”

✓ located verbatim in the source · title, characters 0–106 · show in text ↑
e4

“The results of glycosyl residue and glycosyl linkage composition analyses of the RG-II from control and B-deficient plants were similar”

✓ located verbatim in the source · abstract, characters 369–504 · show in text ↑
Intervention effectauthor interpretationoriginal resultno effectmeasured

Claim · model-written summary

Boron deficiency does not alter the primary structure of RG-II in pumpkin.

Taxon
Pumpkint0
Treatment
B deficiencye5
Outcome
primary structure of RG-IIe5
Direction
does not altere5

Not reportedComparator

Verbatim

t0

“Formation of Rhamnogalacturonan II-Borate Dimer in Pectin Determines Cell Wall Thickness of Pumpkin Tissue”

✓ located verbatim in the source · title, characters 0–106 · show in text ↑
e5

“Thus, B deficiency does not alter the primary structure of RG-II”

✓ located verbatim in the source · abstract, characters 506–570 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Adding 10B-enriched boric acid to boron-deficient pumpkin plants converted mRG-II to dRG-II-10B within 5 h.

Taxon
Pumpkint0
Treatment
addition of (10)B-enriched boric acide6
Outcome
conversion of mRG-II to dRG-II-(10)Be6
Direction
increaseinferrede6
Qualifier
within 5 he6
Comparator
B-deficient plantse6

Verbatim

t0

“Formation of Rhamnogalacturonan II-Borate Dimer in Pectin Determines Cell Wall Thickness of Pumpkin Tissue”

✓ located verbatim in the source · title, characters 0–106 · show in text ↑
e6

“The addition of (10)B-enriched boric acid to B-deficient plants resulted within 5 h in the conversion of mRG-II to dRG-II-(10)B”

✓ located verbatim in the source · abstract, characters 572–699 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

Wall thickness of (10)B-treated pumpkin plants was similar to that of control plants.

Taxon
Pumpkint0
Treatment
(10)B-treated plantse7
Outcome
wall thicknesse7
Direction
similare7
Comparator
control plantse7

Verbatim

t0

“Formation of Rhamnogalacturonan II-Borate Dimer in Pectin Determines Cell Wall Thickness of Pumpkin Tissue”

✓ located verbatim in the source · title, characters 0–106 · show in text ↑
e7

“The wall thickness of the (10)B-treated plants and control plants was similar”

✓ located verbatim in the source · abstract, characters 701–778 · show in text ↑

boron atom · growth · Cucurbita (pumpkins)Results agree

5 papers: 5 positive, 0 negative or no effect

4 papers in the same direction as this paper

This paper is about lacking the nutrient, so it counts the other way round here.

  1. positive
  2. positive
  3. positive

1 paper more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.