Abstract
Plants raised from magnesium nitrate [Mg(NO3)2] primed seeds showed stress ameliorating characters. On the basis of this, during the study of somatic embryogenesis a comparative characterization of the calli were made by using the dehusked, sterilized whole rice (var-Swarna/MTU-7029) seeds, obtained from Mg(NO3)2 primed and non-primed one. For callus induction 30gL−1 sucrose + 1gL−1 casein hydrolysate and 30gL−1 maltose + 1gL−1 casein hydrolysate were added in MS medium separately which was supplemented with 2 mgL−1 2,4-D. In the regeneration medium different concentration of BAP, kinetin and NAA were used. For the regeneration of roots 1mgL−1 IBA was used. Different concentrations (2–8 mM) of the Mg(NO3)2 used for priming before using the seeds on culture medium, showed promising results regarding the high callus induction frequency with faster greening ability and stout shoots in respect to non primed one. 4 mM of Mg(NO3)2 was found best among all nitrate treatment. The fresh and dry weights and proline content were found maximum in the calli obtained from the 4 mM Mg(NO3)2 primed seeds. The Mg(NO3)2 primed sets have also more number of roots reflected more rooting ability in respect to non- primed one.
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Claims 6
Claim · model-written summary
Magnesium nitrate priming increased rice callus induction frequency relative to nonprimed seeds.
- Taxon
- rice
Oryza sativae1 - Material
- var-Swarna/MTU-7029e1
- Treatment
- Mg(NO3)2 used for priminge0
- Outcome
- callus induction frequencye0
- Direction
- highe0
- Value
- 2–8 mMe0
- Comparator
- non primed onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Different concentrations (2–8 mM) of the Mg(NO3)2 used for priming before using the seeds on culture medium, showed promising results regarding the high callus induction frequency with faster greening ability and stout shoots in respect to non primed one.”
e1“whole rice (var-Swarna/MTU-7029) seeds”
Claim · model-written summary
Priming produced faster greening and stout shoots compared with nonprimed seeds.
- Taxon
- riceOryza sativae1
- Material
- var-Swarna/MTU-7029e1
- Treatment
- Mg(NO3)2 used for priminge0
- Outcome
- faster greening ability and stout shootse0
- Direction
- fastere0
- Comparator
- non primed onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Different concentrations (2–8 mM) of the Mg(NO3)2 used for priming before using the seeds on culture medium, showed promising results regarding the high callus induction frequency with faster greening ability and stout shoots in respect to non primed one.”
e1“whole rice (var-Swarna/MTU-7029) seeds”
Claim · model-written summary
The 4 mM magnesium nitrate priming treatment performed best among nitrate treatments.
- Taxon
- riceOryza sativae1
- Treatment
- 4 mM of Mg(NO3)2e0
- Outcome
- beste0
- Direction
- beste0
- Comparator
- all nitrate treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“4 mM of Mg(NO3)2 was found best among all nitrate treatment.”
e1“whole rice (var-Swarna/MTU-7029) seeds”
Claim · model-written summary
Fresh and dry callus weights were maximal after 4 mM magnesium nitrate seed priming.
- Taxon
- riceOryza sativae1
- Treatment
- 4 mM Mg(NO3)2 primed seedse0
- Outcome
- fresh and dry weightse0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fresh and dry weights and proline content were found maximum in the calli obtained from the 4 mM Mg(NO3)2 primed seeds.”
e1“whole rice (var-Swarna/MTU-7029) seeds”
Claim · model-written summary
Callus proline content was maximal after 4 mM magnesium nitrate seed priming.
- Taxon
- riceOryza sativae1
- Treatment
- 4 mM Mg(NO3)2 primed seedse0
- Outcome
- proline contente0
- Direction
- maximume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The fresh and dry weights and proline content were found maximum in the calli obtained from the 4 mM Mg(NO3)2 primed seeds.”
e1“whole rice (var-Swarna/MTU-7029) seeds”
Claim · model-written summary
Magnesium nitrate priming produced more roots than nonpriming.
- Taxon
- riceOryza sativae1
- Treatment
- Mg(NO3)2 primed setse0
- Outcome
- number of rootse0
- Direction
- moree0
- Comparator
- non- primed onee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Mg(NO3)2 primed sets have also more number of roots reflected more rooting ability in respect to non- primed one.”
e1“whole rice (var-Swarna/MTU-7029) seeds”
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See all 26 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.