Abstract
SUMMARY DspA/E is a pathogenicity factor of Erwinia amylovora that is translocated into the plant cell cytoplasm through an Hrp type III secretion system. Transient expression of dspA/E in Nicotiana benthamiana or yeast induced cell death, as it does in N. tabacum and apple as described previously. DspA/E-induced cell death in N. benthamiana was not inhibited by coexpression of AvrPtoB of Pseudomonas syringae pv. tomato, which inhibits programmed cell death (PCD) induced by several other elicitors in plants. Silencing of NbSGT1, the expression of which is required for PCD mediated by several resistance proteins of plants, prevented DspA/E-induced cell death in N. benthamiana. However, silencing of NbRAR1, or two MAP kinase kinase genes, which are required for PCD associated with many resistance genes in plants, did not prevent cell death induced by DspA/E. Silencing of NbSGT1 also compromised non-host resistance against E. amylovora. E. amylovora grew rapidly within the first 24 h after infiltration in N. benthamiana, and DspA/E was required for this early rapid growth. However, bacterial cell numbers decreased after 24 h in TRV-vector-transformed plants, whereas a dspA/E mutant strain grew to high populations in NbSGT1-silenced plants. Our results indicate that DspA/E enhances virulence of E. amylovora in N. benthamiana, but the bacteria are then recognized by the plant, resulting in PCD and death of bacterial cells or restriction of bacterial cell growth.
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Claims 9
Claim · model-written summary
Coexpressing AvrPtoB did not inhibit DspA/E-induced cell death in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianat1 - Treatment
- coexpression of AvrPtoBe2
- Interactor
- Erwinia amylovora
Erwinia amylovorat0pathogen - Second interactor
- Pseudomonas syringae pv. tomato
Pseudomonas syringae pv. tomatoe2pathogen - Outcome
- DspA/E-induced cell deathe2
- Direction
- not inhibitede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“a type III effector of Erwinia amylovora”
t1“is required for early rapid growth in Nicotiana benthamiana”
e2“DspA/E-induced cell death in N. benthamiana was not inhibited by coexpression of AvrPtoB of Pseudomonas syringae pv. tomato”
Claim · model-written summary
Silencing NbSGT1 prevented DspA/E-induced cell death in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Treatment
- Silencing of NbSGT1e3
- Gene
- NbSGT1e3
- Outcome
- DspA/E-induced cell deathe3
- Direction
- preventede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“is required for early rapid growth in Nicotiana benthamiana”
e3“Silencing of NbSGT1, the expression of which is required for PCD mediated by several resistance proteins of plants, prevented DspA/E-induced cell death in N. benthamiana”
Claim · model-written summary
Silencing NbRAR1 or two MAP kinase kinase genes did not prevent DspA/E-induced cell death in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Treatment
- silencing of NbRAR1, or two MAP kinase kinase genese4
- Gene
- NbRAR1e4
- Outcome
- cell death induced by DspA/Ee4
- Direction
- did not prevente4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“is required for early rapid growth in Nicotiana benthamiana”
e4“silencing of NbRAR1, or two MAP kinase kinase genes, which are required for PCD associated with many resistance genes in plants, did not prevent cell death induced by DspA/E”
Claim · model-written summary
Silencing NbSGT1 compromised non-host resistance of Nicotiana benthamiana against Erwinia amylovora.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Treatment
- Silencing of NbSGT1e5
- Interactor
- E. amylovoraErwinia amylovorae5pathogen
- Gene
- NbSGT1e5
- Outcome
- non-host resistance against E. amylovorae5
- Direction
- compromisede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“is required for early rapid growth in Nicotiana benthamiana”
e5“Silencing of NbSGT1 also compromised non-host resistance against E. amylovora”
Claim · model-written summary
Erwinia amylovora grew rapidly within the first 24 h in Nicotiana benthamiana, and DspA/E was required for this growth.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Interactor
- E. amylovoraErwinia amylovorae6pathogen
- Outcome
- early rapid growthe6
- Qualifier
- DspA/E was requirede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“is required for early rapid growth in Nicotiana benthamiana”
e6“E. amylovora grew rapidly within the first 24 h after infiltration in N. benthamiana, and DspA/E was required for this early rapid growth”
Claim · model-written summary
Bacterial cell numbers decreased after 24 h in TRV-vector-transformed Nicotiana benthamiana plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Outcome
- bacterial cell numberse7
- Direction
- decreasede7
- Qualifier
- after 24 h in TRV-vector-transformed plantse7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“is required for early rapid growth in Nicotiana benthamiana”
e7“However, bacterial cell numbers decreased after 24 h in TRV-vector-transformed plants”
Claim · model-written summary
A dspA/E mutant strain grew to high populations in NbSGT1-silenced Nicotiana benthamiana plants.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Material
- NbSGT1-silenced plantse0
- Interactor
- dspA/E mutant straine0pathogen
- Outcome
- grew to high populationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“is required for early rapid growth in Nicotiana benthamiana”
e0“whereas a dspA/E mutant strain grew to high populations in NbSGT1-silenced plants”
Claim · model-written summary
Nicotiana benthamiana recognizes the bacteria, resulting in programmed cell death and restriction of bacterial growth.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Outcome
- death of bacterial cells or restriction of bacterial cell growthe1
- Qualifier
- recognized by the plant, resulting in PCDe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“is required for early rapid growth in Nicotiana benthamiana”
e1“the bacteria are then recognized by the plant, resulting in PCD and death of bacterial cells or restriction of bacterial cell growth”
Claim · model-written summary
DspA/E enhances the virulence of Erwinia amylovora in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamianaNicotiana benthamianat1
- Interactor
- E. amylovoraErwinia amylovorae0pathogen
- Outcome
- virulence of E. amylovorae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t1“is required for early rapid growth in Nicotiana benthamiana”
e0“Our results indicate that DspA/E enhances virulence of E. amylovora in N. benthamiana”
The same questions in other papers
loss of function · cytology · Nicotiana benthamianaPositive and negative results
23 papers in the same direction as this paper · 9 in the opposite direction
- positive
- positive
- positive
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See all 33 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.