Paper · 1946 · Botanical Gazette

Effect of Spray Applications of 2,4-Dichlorophenoxyacetic Acid on Subsequent Growth of Various Parts of Red Kidney Bean and Soybean Plants

Robert J. Weaver

20
claims
35
verbatim quotes
5
citations
2
research areas
—
Not open access

Abstract

1. Plots containing young red kidney bean and soybean plants were sprayed with 2,4-dichlorophenoxyacetic acid at rates of 0.1, 0.01, and 0.001 gm. per sq. yd. The effects of the agent on the growth of various plant parts were studied. Linear, and fresh and dry weight measurements were obtained at several intervals following treatment. 2. Hypocotyls and first internodes increased greatly in diameter. The increase in fresh weight was due mostly to increased water content. 3. Primary leaves of treated plants were much heavier than those of controls. Those of red kidney beans remained green and attached to the plants long after those of controls had withered and fallen. 4. Total weights of trifoliate leaf blades, trifoliate leaves, and weight of plants above second node were decreased by the growth regulator. There was good correlation between decrease in weight and increase in amount of compound applied. These criteria would be excellent in studying the relative inhibitory or stimulating actions of growth regulators on red kidney beans. Ten days after treatment there were highly significant differences between controls and treated plants, and between treated plants, when fresh weights of trifoliate leaves or leaf blades were used as criteria. The differences were significant when the fresh weight of kidney bean plants above the second node was used as a criterion. With soybeans, only fresh weight of trifoliate leaf blades gave significant differences between controls and treated plants and between plants that received various rates of compound when plants were harvested 21 days after treatment. 5. The growth in length and width of certain leaflets which developed after treatment was inhibited by the growth regulator. The two higher levels of compound (0.1 or 0.01 gm. per sq. yd.) inhibited the linear growth of certain petioles. The lowest level (0.001 gm. per sq. yd.) stimulated growth in length of a soybean petiole but had little effect on a bean petiole. 6 The fresh weight of tops was decreased by the growth regulator. Heights were usually decreased but differences caused by various treatments were sometimes small. 7. The compound at rates of 0.01 and 0.1 gm. per sq. yd. decreased dry weight of roots of kidney bean, but 0.001 gm. had little effect. With soybeans the two lower rates of application significantly increased weight of roots at 16 days after treatment. However, by 21 days the weight of control roots was about the same as that of the treated plants. 8. With kidney beans there was little effect on the shoot-to-root ratio when two lower amounts of the compound were used. With soybeans, the ratio was reduced by all treatments. 9. The growth regulator delayed the onset and decreased the amount of pod formation.

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Claims 20

Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Hypocotyl and internode diameter increased.

Taxon
Red Kidney Bean and Soybean Plantst0
Treatment
2,4-dichlorophenoxyacetic acide1
Outcome
Hypocotyls and first internodese0
Direction
increasede0

Not reportedComparator

Verbatim

e0

“Hypocotyls and first internodes increased greatly in diameter”

✓ located verbatim in the source · abstract, characters 340–401 · show in text ↑
t0

“Red Kidney Bean and Soybean Plants”

✓ located verbatim in the source · title, characters 104–138 · show in text ↑
e1

“sprayed with 2,4-dichlorophenoxyacetic acid”

✓ located verbatim in the source · abstract, characters 66–109 · show in text ↑
Mechanismobservedoriginal resultmeasured

Claim · model-written summary

Increased fresh weight was mostly due to water content.

Taxon
Red Kidney Bean and Soybean Plantst0
Outcome
increase in fresh weighte0
Qualifier
increased water contente0

Verbatim

e0

“The increase in fresh weight was due mostly to increased water content”

✓ located verbatim in the source · abstract, characters 403–473 · show in text ↑
t0

“Red Kidney Bean and Soybean Plants”

✓ located verbatim in the source · title, characters 104–138 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Treated primary leaves were heavier than controls.

Taxon
Red Kidney Bean and Soybean Plantst0
Treatment
2,4-dichlorophenoxyacetic acide1
Outcome
Primary leavese0
Direction
heaviere0
Comparator
controlse0

Verbatim

e0

“Primary leaves of treated plants were much heavier than those of controls”

✓ located verbatim in the source · abstract, characters 478–551 · show in text ↑
t0

“Red Kidney Bean and Soybean Plants”

✓ located verbatim in the source · title, characters 104–138 · show in text ↑
e1

“sprayed with 2,4-dichlorophenoxyacetic acid”

✓ located verbatim in the source · abstract, characters 66–109 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Treated kidney bean leaves stayed green and attached longer.

Taxon
red kidney beanse0
Treatment
2,4-dichlorophenoxyacetic acide1
Outcome
remained green and attached to the plantse0
Comparator
controlse0

Not reportedDirection

Verbatim

e0

“Those of red kidney beans remained green and attached to the plants long after those of controls had withered and fallen”

✓ located verbatim in the source · abstract, characters 553–673 · show in text ↑
e1

“sprayed with 2,4-dichlorophenoxyacetic acid”

✓ located verbatim in the source · abstract, characters 66–109 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The regulator decreased trifoliate blades, leaves and above-node weight.

Taxon
Red Kidney Bean and Soybean Plantst0
Treatment
growth regulatore0
Outcome
Total weights of trifoliate leaf blades, trifoliate leaves, and weight of plants above second nodee0
Direction
decreasede0

Not reportedComparator

Verbatim

e0

“Total weights of trifoliate leaf blades, trifoliate leaves, and weight of plants above second node were decreased by the growth regulator”

✓ located verbatim in the source · abstract, characters 678–815 · show in text ↑
t0

“Red Kidney Bean and Soybean Plants”

✓ located verbatim in the source · title, characters 104–138 · show in text ↑
Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Greater dose correlated with greater weight reduction.

Taxon
Red Kidney Bean and Soybean Plantst0
Treatment
compound appliede0
Outcome
decrease in weighte0
Direction
decreasee0

Verbatim

e0

“There was good correlation between decrease in weight and increase in amount of compound applied”

✓ located verbatim in the source · abstract, characters 817–913 · show in text ↑
t0

“Red Kidney Bean and Soybean Plants”

✓ located verbatim in the source · title, characters 104–138 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

At ten days kidney bean trifoliate leaf weights differed significantly among controls and treatments.

Taxon
red kidney beanse1
Treatment
treatmente0
Outcome
fresh weights of trifoliate leaves or leaf bladese0
Qualifier
Ten days after treatmente0
Comparator
controlse0

Not reportedDirection

Verbatim

e0

“Ten days after treatment there were highly significant differences between controls and treated plants, and between treated plants, when fresh weights of trifoliate leaves or leaf blades were used as criteria”

✓ located verbatim in the source · abstract, characters 1050–1258 · show in text ↑
e1

“red kidney beans remained green”

✓ located verbatim in the source · abstract, characters 562–593 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

Kidney bean above-node fresh weight differed significantly.

Taxon
kidney beanPhaseolus vulgarise0
Outcome
fresh weight of kidney bean plants above the second nodee0

Not reportedTreatmentComparatorDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The differences were significant when the fresh weight of kidney bean plants above the second node was used as a criterion”

✓ located verbatim in the source · abstract, characters 1260–1382 · show in text ↑
Intervention effectobservedoriginal resultsignificant (as stated)measured

Claim · model-written summary

In soybean only trifoliate blade fresh weight differed significantly at day 21.

Taxon
soybeansGlycine maxe0
Treatment
various rates of compounde0
Outcome
fresh weight of trifoliate leaf bladese0
Qualifier
21 days after treatmente0
Comparator
controlse0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“With soybeans, only fresh weight of trifoliate leaf blades gave significant differences between controls and treated plants and between plants that received various rates of compound when plants were harvested 21 days after treatment”

✓ located verbatim in the source · abstract, characters 1384–1617 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The regulator inhibited leaflet length and width growth.

Taxon
Red Kidney Bean and Soybean Plantst0
Treatment
growth regulatore0
Outcome
growth in length and width of certain leafletse0
Direction
inhibitede0

Not reportedComparator

Verbatim

e0

“The growth in length and width of certain leaflets which developed after treatment was inhibited by the growth regulator”

✓ located verbatim in the source · abstract, characters 1622–1742 · show in text ↑
t0

“Red Kidney Bean and Soybean Plants”

✓ located verbatim in the source · title, characters 104–138 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The two higher doses inhibited petiole linear growth.

Taxon
Red Kidney Bean and Soybean Plantst0
Treatment
0.1 or 0.01 gm. per sq. yd.e0
Outcome
linear growth of certain petiolese0
Direction
inhibitede0

Not reportedComparator

Verbatim

e0

“The two higher levels of compound (0.1 or 0.01 gm. per sq. yd.) inhibited the linear growth of certain petioles”

✓ located verbatim in the source · abstract, characters 1744–1855 · show in text ↑
t0

“Red Kidney Bean and Soybean Plants”

✓ located verbatim in the source · title, characters 104–138 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The lowest dose stimulated soybean petiole length.

Taxon
soybeanGlycine maxe0
Treatment
0.001 gm. per sq. yd.e0
Outcome
growth in length of a soybean petiolee0
Direction
stimulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“The lowest level (0.001 gm. per sq. yd.) stimulated growth in length of a soybean petiole”

✓ located verbatim in the source · abstract, characters 1857–1946 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

The lowest dose had little effect on bean petiole length.

Taxon
beane0
Treatment
0.001 gm. per sq. yd.e0
Outcome
bean petiolee0
Direction
little effecte0

Not reportedComparator

Verbatim

e0

“The lowest level (0.001 gm. per sq. yd.) stimulated growth in length of a soybean petiole but had little effect on a bean petiole”

✓ located verbatim in the source · abstract, characters 1857–1986 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The regulator decreased top fresh weight and usually plant height.

Taxon
Red Kidney Bean and Soybean Plantst0
Treatment
growth regulatore0
Outcome
fresh weight of topse0
Direction
decreasede0

Not reportedComparator

Verbatim

e0

“The fresh weight of tops was decreased by the growth regulator. Heights were usually decreased”

✓ located verbatim in the source · abstract, characters 1990–2084 · show in text ↑
t0

“Red Kidney Bean and Soybean Plants”

✓ located verbatim in the source · title, characters 104–138 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The two higher rates decreased kidney bean root dry weight.

Taxon
kidney beanPhaseolus vulgarise0
Treatment
0.01 and 0.1 gm. per sq. yd.e0
Outcome
dry weight of rootse0
Direction
decreasede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The compound at rates of 0.01 and 0.1 gm. per sq. yd. decreased dry weight of roots of kidney bean”

✓ located verbatim in the source · abstract, characters 2155–2253 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

The lowest rate had little effect on kidney bean roots.

Taxon
kidney beanPhaseolus vulgarise1
Treatment
0.001 gm.e0
Outcome
dry weight of rootse1
Direction
little effecte0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“but 0.001 gm. had little effect”

✓ located verbatim in the source · abstract, characters 2255–2286 · show in text ↑
e1

“dry weight of roots of kidney bean”

✓ located verbatim in the source · abstract, characters 2219–2253 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

Two lower rates increased soybean root weight at 16 days.

Taxon
soybeansGlycine maxe0
Treatment
two lower rates of applicatione0
Outcome
weight of rootse0
Direction
increasede0
Qualifier
16 days after treatmente0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“With soybeans the two lower rates of application significantly increased weight of roots at 16 days after treatment”

✓ located verbatim in the source · abstract, characters 2288–2403 · show in text ↑
Intervention effectobservedoriginal resultno effectmeasured

Claim · model-written summary

By day 21 soybean control and treated root weights were similar.

Taxon
soybeansGlycine maxe1
Treatment
treated plantse0
Outcome
weight of control rootse0
Direction
about the samee0
Qualifier
by 21 dayse0
Comparator
control rootse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Glycine max
Glycine max · TaxonPublic domain · Wikimedia Commons ↗

Verbatim

e0

“by 21 days the weight of control roots was about the same as that of the treated plants”

✓ located verbatim in the source · abstract, characters 2414–2501 · show in text ↑
e1

“With soybeans the two lower rates of application”

✓ located verbatim in the source · abstract, characters 2288–2336 · show in text ↑
Observationobservedoriginal resultno effectmeasured

Claim · model-written summary

Bean shoot-to-root ratio changed little at lower doses while soybean ratio declined at all doses.

Taxon
kidney beansPhaseolus vulgarise0
Outcome
shoot-to-root ratioe0
Direction
little effecte0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Phaseolus vulgaris
Phaseolus vulgaris · TaxonRasbak · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“With kidney beans there was little effect on the shoot-to-root ratio when two lower amounts of the compound were used. With soybeans, the ratio was reduced by all treatments”

✓ located verbatim in the source · abstract, characters 2506–2679 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

The regulator delayed and reduced pod formation.

Taxon
Red Kidney Bean and Soybean Plantst0
Treatment
growth regulatore0
Outcome
pod formatione0
Direction
decreasede0

Not reportedComparator

Verbatim

e0

“The growth regulator delayed the onset and decreased the amount of pod formation”

✓ located verbatim in the source · abstract, characters 2684–2764 · show in text ↑
t0

“Red Kidney Bean and Soybean Plants”

✓ located verbatim in the source · title, characters 104–138 · show in text ↑

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62 papers: 47 positive, 15 negative or no effect

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.