Abstract
We have investigated the effect of aluminum (Al) on the activity of glucose‐6‐phosphate dehydrogenase (G6PDH; EC 1.1.1.49) and 6‐phosphogluconate dehydrogenase (6PGDH; EC 1.1.1.44) isolated from 5‐mm root apices of 4‐day‐old wheat ( Triticum aestivum ) cultivars differing in resistance to Al. Rapid increases in G6PDH and 6PGDH activities were observed in Al‐resistant cultivars (PT741 and Atlas 66) during the first 10 h of treatment with 100 μ M Al, while no change in the activity of either enzyme was observed in Al‐sensitive cultivars (Katepwa and Neepawa) during a 24‐h exposure to Al. The Al‐induced increases in enzyme activities observed in the Al‐resistant PT741 appear to reflect an induction of protein synthesis since the increases were completely abolished by 1 m M cycloheximide. No differences in G6PDH and 6PGDH activities were observed between the Al‐sensitive and the Al‐resistant genotypes when Al was supplied in vitro. Under these conditions, an increase in Al concentration from 0 to 1.4 m M caused a gradual decrease in activity of both enzymes, irrespective of the Al‐resistance of whole seedlings. Aluminum‐sensitive and aluminum‐resistant cultivars also differed in the rate and extent of accumulation of slowly‐exchanging Al in 5‐mm root apices. During the first 6 h of Al treatment, Al accumulation was only 10% more rapid in Katepwa than in PT741. After 24‐h exposure, accumulation in the Al‐sensitive Katepwa, was two‐fold higher. A decline in Al accumulation in a slowly‐exchanging compartment as well as a decrease in activities of G6PDH and 6PGDH were found in the Al‐resistant PT741, when seedlings were transferred to Al‐free treatment solutions after 16‐h exposure to 100 μ M Al. These results suggest that rapid induction of G6PDH and 6PGDH in the Al‐resistant line PT741 by Al may play a role in the mechanism of Al resistance, possibly by regulation of the pentose phosphate pathway.
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Claims 10
Claim · model-written summary
Al increased G6PDH and 6PGDH activities in resistant wheat cultivars during the first 10 hours.
- Taxon
- wheat
Triticum aestivumt0 - Material
- PT741 and Atlas 66e0
- Treatment
- 100 μ M Ale0
- Gene
- G6PDH and 6PGDHe0
- Outcome
- G6PDH and 6PGDH activitiese0
- Direction
- Rapid increasese0
- Qualifier
- first 10 he0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rapid increases in G6PDH and 6PGDH activities were observed in Al‐resistant cultivars (PT741 and Atlas 66) during the first 10 h of treatment with 100 μ M Al”
t0“wheat”
Claim · model-written summary
Al did not change either enzyme activity in sensitive wheat cultivars over 24 hours.
- Taxon
- wheatTriticum aestivumt0
- Material
- Katepwa and Neepawae0
- Treatment
- Ale0
- Outcome
- activity of either enzymee0
- Direction
- no changee0
- Qualifier
- 24‐h exposuree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“no change in the activity of either enzyme was observed in Al‐sensitive cultivars (Katepwa and Neepawa) during a 24‐h exposure to Al”
t0“wheat”
Claim · model-written summary
Cycloheximide abolished Al-induced enzyme activity increases in PT741.
- Taxon
- wheatTriticum aestivumt0
- Material
- PT741e0
- Treatment
- 1 m M cycloheximidee0
- Outcome
- increases in enzyme activitiese0
- Direction
- completely abolishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Al‐induced increases in enzyme activities observed in the Al‐resistant PT741 appear to reflect an induction of protein synthesis since the increases were completely abolished by 1 m M cycloheximide.”
t0“wheat”
Claim · model-written summary
In vitro Al produced no enzyme activity difference between resistant and sensitive wheat genotypes.
- Taxon
- wheatTriticum aestivumt0
- Gene
- G6PDH and 6PGDHe0
- Outcome
- No differences in G6PDH and 6PGDH activitiese0
- Comparator
- Al‐sensitive and the Al‐resistant genotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No differences in G6PDH and 6PGDH activities were observed between the Al‐sensitive and the Al‐resistant genotypes when Al was supplied in vitro.”
t0“wheat”
Claim · model-written summary
Increasing in vitro Al decreased both enzyme activities regardless of seedling resistance.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Al concentration from 0 to 1.4 m Me0
- Outcome
- activity of both enzymese0
- Direction
- gradual decreasee0
- Comparator
- 0e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in Al concentration from 0 to 1.4 m M caused a gradual decrease in activity of both enzymes, irrespective of the Al‐resistance of whole seedlings.”
t0“wheat”
Claim · model-written summary
Al accumulation in Katepwa was 10% more rapid than PT741 over the first 6 hours.
- Taxon
- wheatTriticum aestivumt0
- Material
- Katepwae0
- Outcome
- Al accumulatione0
- Direction
- more rapide0
- Value
- 10%e0
- Comparator
- PT741e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“During the first 6 h of Al treatment, Al accumulation was only 10% more rapid in Katepwa than in PT741.”
t0“wheat”
Claim · model-written summary
After 24 hours, Al accumulation in Katepwa was two-fold higher than PT741.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“After 24‐h exposure, accumulation in the Al‐sensitive Katepwa, was two‐fold higher.”
t0“wheat”
Claim · model-written summary
Transfer of PT741 to Al-free solution reduced slowly exchanging Al accumulation.
- Taxon
- wheatTriticum aestivumt0
- Material
- PT741e0
- Treatment
- Al‐free treatment solutionse0
- Outcome
- Al accumulation in a slowly‐exchanging compartmente0
- Direction
- declinee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A decline in Al accumulation in a slowly‐exchanging compartment as well as a decrease in activities of G6PDH and 6PGDH were found in the Al‐resistant PT741, when seedlings were transferred to Al‐free treatment solutions after 16‐h exposure to 100 μ M Al.”
t0“wheat”
Claim · model-written summary
Transfer of PT741 to Al-free solution reduced G6PDH and 6PGDH activities.
- Taxon
- wheatTriticum aestivumt0
- Material
- PT741e0
- Treatment
- Al‐free treatment solutionse0
- Gene
- G6PDH and 6PGDHe0
- Outcome
- activities of G6PDH and 6PGDHe0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A decline in Al accumulation in a slowly‐exchanging compartment as well as a decrease in activities of G6PDH and 6PGDH were found in the Al‐resistant PT741, when seedlings were transferred to Al‐free treatment solutions after 16‐h exposure to 100 μ M Al.”
t0“wheat”
Claim · model-written summary
The authors suggest rapid induction of G6PDH and 6PGDH may contribute to PT741 aluminum resistance through pentose phosphate pathway regulation.
- Taxon
- wheatTriticum aestivumt0
- Gene
- G6PDH and 6PGDHe0
- Outcome
- Al resistancee0
- Qualifier
- possibly by regulation of the pentose phosphate pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rapid induction of G6PDH and 6PGDH in the Al‐resistant line PT741 by Al may play a role in the mechanism of Al resistance, possibly by regulation of the pentose phosphate pathway.”
t0“wheat”
The same questions in other papers
aluminium atom · physiology: enzyme activity · Triticum aestivum (wheat)Results agree
8 papers in the same direction as this paper
- no effect
- negative
- negative
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.