Paper · 2014 · American Journal of Experimental Agriculture

Effects of Phosphorus and Bradyrhizobium japonicum on Growth and Chlorophyll Content of Cowpea (Vigna unguiculata (L) Walp)

Daniel Nyoki

11
claims
28
verbatim quotes
36
citations
2
research areas
✓
Open access

Abstract

The field and screen house experiment was conducted at Tanzania Coffee Research Institute and Seliani Agricultural Research Institute respectively in Tanzania, between March-July 2013, to assess the effects of Bradyrhizobium japonicum and phosphorus on growth and total leaf chlorophyll content of cowpea. The experiment was out down in splitplot design whereby the main plots comprised two inoculation treatments (with and without B. japonicum), while the sub-plots contained phosphorus (TSP) application at four different levels (0 kg/ha, 20 kg/ha, 40 kg/ha, 80 kg/ha). Both experiments were replicated four times. We measured plant growth parameters such as plant height; number of leaves per plant at different stages of plant growth, the stem girth was measured by vernier caliper at physiological maturity. The chlorophyll content was determined at 3, 5, and 7 weeks after planting (WAP). The chlorophyll was extracted by using dimethylsulphoxide (DMSO) and absorbance was determined at 645 and 663nm using UV/Visible spectrophotometer. B. japonicum inoculation significantly increased the plant height, number of leaves per plants, and stem girth above the control. The height was increased by 11.23, 10.43 and 8.99% (screen-house) and by 8.11, 24.05, 9.29% (field) in the measurements taken at 4, 6, and 8 WAP respectively. Number of leaves per plant counted at 6 and 8 WAP increased by 14 and 10.8% (screen-house) and 14 and 11.6% (field) respectively. B. japonicum also

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Claims 11

Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

B. japonicum inoculation significantly increased height, leaf number and stem girth.

Treatment
B. japonicum inoculatione0
Interactor
B. japonicumBradyrhizobium japonicume0symbiont
Outcome
plant height, number of leaves per plants, and stem girthe0
Direction
increasede0
Comparator
controle0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗
Bradyrhizobium japonicum
Bradyrhizobium japonicum · InteractorLouisa Howard - Dartmouth Electron Microscope Facility · Public domain · Wikimedia Commons ↗

Verbatim

e0

“B. japonicum inoculation significantly increased the plant height, number of leaves per plants, and stem girth above the control.”

✓ located verbatim in the source · abstract, characters 1043–1172 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased cowpea height by 11.23 at screen-house.

Treatment
inoculatione1
Outcome
heighte0
Direction
increasede0
Value
11.23e0
Setting
screen-housee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The height was increased by 11.23, 10.43 and 8.99% (screen-house) and by 8.11, 24.05, 9.29% (field) in the measurements taken at 4, 6, and 8 WAP respectively.”

✓ located verbatim in the source · abstract, characters 1173–1331 · show in text ↑
e1

“B. japonicum inoculation significantly increased the plant height, number of leaves per plants, and stem girth above the control.”

✓ located verbatim in the source · abstract, characters 1043–1172 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased cowpea height by 10.43 at screen-house.

Treatment
inoculatione1
Outcome
heighte0
Direction
increasede0
Value
10.43e0
Setting
screen-housee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The height was increased by 11.23, 10.43 and 8.99% (screen-house) and by 8.11, 24.05, 9.29% (field) in the measurements taken at 4, 6, and 8 WAP respectively.”

✓ located verbatim in the source · abstract, characters 1173–1331 · show in text ↑
e1

“B. japonicum inoculation significantly increased the plant height, number of leaves per plants, and stem girth above the control.”

✓ located verbatim in the source · abstract, characters 1043–1172 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased cowpea height by 8.99% at screen-house.

Treatment
inoculatione1
Outcome
heighte0
Direction
increasede0
Value
8.99%e0
Setting
screen-housee0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The height was increased by 11.23, 10.43 and 8.99% (screen-house) and by 8.11, 24.05, 9.29% (field) in the measurements taken at 4, 6, and 8 WAP respectively.”

✓ located verbatim in the source · abstract, characters 1173–1331 · show in text ↑
e1

“B. japonicum inoculation significantly increased the plant height, number of leaves per plants, and stem girth above the control.”

✓ located verbatim in the source · abstract, characters 1043–1172 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased cowpea height by 8.11 at field.

Treatment
inoculatione1
Outcome
heighte0
Direction
increasede0
Value
8.11e0
Setting
fielde0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The height was increased by 11.23, 10.43 and 8.99% (screen-house) and by 8.11, 24.05, 9.29% (field) in the measurements taken at 4, 6, and 8 WAP respectively.”

✓ located verbatim in the source · abstract, characters 1173–1331 · show in text ↑
e1

“B. japonicum inoculation significantly increased the plant height, number of leaves per plants, and stem girth above the control.”

✓ located verbatim in the source · abstract, characters 1043–1172 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased cowpea height by 24.05 at field.

Treatment
inoculatione1
Outcome
heighte0
Direction
increasede0
Value
24.05e0
Setting
fielde0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The height was increased by 11.23, 10.43 and 8.99% (screen-house) and by 8.11, 24.05, 9.29% (field) in the measurements taken at 4, 6, and 8 WAP respectively.”

✓ located verbatim in the source · abstract, characters 1173–1331 · show in text ↑
e1

“B. japonicum inoculation significantly increased the plant height, number of leaves per plants, and stem girth above the control.”

✓ located verbatim in the source · abstract, characters 1043–1172 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Inoculation increased cowpea height by 9.29% at field.

Treatment
inoculatione1
Outcome
heighte0
Direction
increasede0
Value
9.29%e0
Setting
fielde0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The height was increased by 11.23, 10.43 and 8.99% (screen-house) and by 8.11, 24.05, 9.29% (field) in the measurements taken at 4, 6, and 8 WAP respectively.”

✓ located verbatim in the source · abstract, characters 1173–1331 · show in text ↑
e1

“B. japonicum inoculation significantly increased the plant height, number of leaves per plants, and stem girth above the control.”

✓ located verbatim in the source · abstract, characters 1043–1172 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Leaf number increased by 14 at screen-house.

Outcome
Number of leaves per plante0
Direction
increasede0
Value
14e0
Setting
screen-housee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Number of leaves per plant counted at 6 and 8 WAP increased by 14 and 10.8% (screen-house) and 14 and 11.6% (field) respectively.”

✓ located verbatim in the source · abstract, characters 1332–1461 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Leaf number increased by 10.8% at screen-house.

Outcome
Number of leaves per plante0
Direction
increasede0
Value
10.8%e0
Setting
screen-housee0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Number of leaves per plant counted at 6 and 8 WAP increased by 14 and 10.8% (screen-house) and 14 and 11.6% (field) respectively.”

✓ located verbatim in the source · abstract, characters 1332–1461 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Leaf number increased by 14 at field.

Outcome
Number of leaves per plante0
Direction
increasede0
Value
14e0
Setting
fielde0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Number of leaves per plant counted at 6 and 8 WAP increased by 14 and 10.8% (screen-house) and 14 and 11.6% (field) respectively.”

✓ located verbatim in the source · abstract, characters 1332–1461 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑
Observationobservedoriginal resultpositivemeasured

Claim · model-written summary

Leaf number increased by 11.6% at field.

Outcome
Number of leaves per plante0
Direction
increasede0
Value
11.6%e0
Setting
fielde0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Vigna unguiculata
Vigna unguiculata · TaxonHeraldDesa at English Wikipedia · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Number of leaves per plant counted at 6 and 8 WAP increased by 14 and 10.8% (screen-house) and 14 and 11.6% (field) respectively.”

✓ located verbatim in the source · abstract, characters 1332–1461 · show in text ↑
t0

“Cowpea (Vigna unguiculata (L) Walp)”

✓ located verbatim in the source · title, characters 88–123 · show in text ↑

inoculation · yield · Vigna unguiculata (cowpea)Results differ

116 papers: 99 positive, 17 negative or no effect

98 papers in the same direction as this paper · 16 in the opposite direction · 1 reporting no difference from an alternative

  1. positive
  2. positive
  3. negative

112 papers more in this group

See all 116 papers →

inoculation · growth · Vigna unguiculata (cowpea)Positive and negative results

102 papers: 92 positive, 10 negative or no effect

91 papers in the same direction as this paper · 8 in the opposite direction · 2 reporting no difference from an alternative

  1. positive
  2. no effect
  3. negative

98 papers more in this group

See all 102 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.