Abstract
Mechanical damage to leaf tissue causes an increase in abscisic acid (ABA) which in turn activates the biosynthesis of jasmonic acid (JA). The resulting higher endogenous JA levels subsequently activate the expression of wound-inducible genes. This study shows that JA induces the expression of different sets of genes in roots and leaves of potato plants. When roots of intact plants were treated with JA, high levels of proteinase inhibitor II (pin2), cathepsin D inhibitor, leucine aminopeptidase and threonine deaminase mRNAs accumulated in the systemic leaves. However, in the treated roots, very low, if any, expression of these genes could be detected. In contrast, a novel, root-specific pin2 homologue accumulated in the JA-treated root tissue which could not be detected in leaves, either systemic or those directly treated with JA. Application of okadaic acid and staurosporine revealed that a protein phosphorylation step is involved in the regulation of this differential response. In leaves, a protein phosphatase is required for the JA-induced expression of pin2 and the other genes analysed. This phosphatase activity is not necessary for the JA-induced expression of a pin2 homologue in roots, suggesting the existence of different transduction pathways for the JA signal in these organs. The requirement of a protein phosphatase activity for JA-mediated gene induction has enabled identification of a JA-independent pathway for ABA induction of pin2 and the other wound-inducible genes. This alternative pathway involves a protein kinase, and appears to be selective for wound-inducible genes. Our data suggest the presence of a complex, organ-specific transduction network for regulating the effects of the plant hormones ABA and JA on gene expression upon wounding.
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Claims 9
Claim · model-written summary
JA induced different gene sets in potato roots and leaves.
- Taxon
- potato
Solanum tuberosume0 - Treatment
- JAe0
- Outcome
- expression of different sets of genese0
- Direction
- inducese0
- Comparator
- roots and leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“JA induces the expression of different sets of genes in roots and leaves of potato plants”
Claim · model-written summary
JA treatment of intact roots led to high pin2, cathepsin D inhibitor, leucine aminopeptidase and threonine deaminase mRNAs in systemic leaves.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- systemic leavese0
- Treatment
- treated with JAe0
- Outcome
- proteinase inhibitor II (pin2), cathepsin D inhibitor, leucine aminopeptidase and threonine deaminase mRNAse0
- Direction
- high levelse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“When roots of intact plants were treated with JA, high levels of proteinase inhibitor II (pin2), cathepsin D inhibitor, leucine aminopeptidase and threonine deaminase mRNAs accumulated in the systemic leaves.”
t0“potato”
Claim · model-written summary
Those JA-responsive mRNAs had very low or undetectable expression in treated roots.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- treated rootse0
- Outcome
- expression of these genese0
- Direction
- very low, if anye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the treated roots, very low, if any, expression of these genes could be detected”
t0“potato”
Claim · model-written summary
A root-specific pin2 homologue accumulated in JA-treated roots but was undetectable in leaves.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- root tissuee0
- Treatment
- JA-treatede0
- Gene
- pin2e0
- Outcome
- root-specific pin2 homologuee0
- Direction
- accumulatede0
- Comparator
- leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel, root-specific pin2 homologue accumulated in the JA-treated root tissue which could not be detected in leaves, either systemic or those directly treated with JA”
t0“potato”
Claim · model-written summary
Phosphorylation participates in regulation of the organ-specific JA response.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- differential responsee0
- Qualifier
- protein phosphorylation stepe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a protein phosphorylation step is involved in the regulation of this differential response”
t0“potato”
Claim · model-written summary
A protein phosphatase is required for JA-induced pin2 and other gene expression in leaves.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- leavese0
- Treatment
- JA-inducede0
- Gene
- pin2e0
- Outcome
- expression of pin2 and the other genes analysede0
- Qualifier
- a protein phosphatase is requirede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In leaves, a protein phosphatase is required for the JA-induced expression of pin2 and the other genes analysed”
t0“potato”
Claim · model-written summary
Phosphatase activity is unnecessary for JA-induced root pin2 homologue expression.
- Taxon
- potatoSolanum tuberosumt0
- Organ / stage
- rootse0
- Gene
- pin2e0
- Outcome
- expression of a pin2 homologuee0
- Qualifier
- phosphatase activity is not necessarye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This phosphatase activity is not necessary for the JA-induced expression of a pin2 homologue in roots”
t0“potato”
Claim · model-written summary
ABA can induce pin2 and other wound genes through a JA-independent pathway.
- Taxon
- potatoSolanum tuberosumt0
- Gene
- pin2e0
- Outcome
- induction of pin2 and the other wound-inducible genese0
- Qualifier
- JA-independent pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a JA-independent pathway for ABA induction of pin2 and the other wound-inducible genes”
t0“potato”
Claim · model-written summary
The alternative ABA pathway involves a protein kinase and appears selective for wound genes.
- Taxon
- potatoSolanum tuberosumt0
- Outcome
- wound-inducible genese0
- Qualifier
- involves a protein kinasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This alternative pathway involves a protein kinase, and appears to be selective for wound-inducible genes”
t0“potato”
The same questions in other papers
jasmonic acid · molecular: metabolites · Solanum tuberosum (potato)Results agree
2 papers in the same direction as this paper
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.