Abstract
Inoculation of leaves of Arabidopsis thaliana (L.) Heynh. with the wheat pathogen, Pseudomonas syringae pv syringae, resulted in the expression of the hypersensitive reaction and in phytoalexin accumulation. No phytoalexin accumulation was detected after infiltration of leaves with a mutant of P. s. syringae deficient in the ability to elicit a hypersensitive reaction; with the crucifer pathogen, Xanthomonas campestris pv campestris; or with 10 millimolar potassium phosphate buffer (pH 6.9). Phytoalexin accumulation was correlated with the restricted in vivo growth of P. s. syringae. A phytoalexin was purified by a combination of reverse phase flash chromatography, thin layer chromatography, followed by reverse phase high performance liquid chromatography. The Arabidopsis phytoalexin was identified as 3-thiazol-2'-yl-indole on the basis of ultraviolet, infrared, mass spectral, (1)H-nuclear magnetic resonance, and (13)C-nuclear magnetic resonance data.
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Claims 6
Claim · model-written summary
Pseudomonas inoculation elicited hypersensitive reaction and phytoalexin accumulation.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Organ / stage
- leavese0
- Treatment
- Inoculation of leaves of Arabidopsis thaliana (L.) Heynh. with the wheat pathogen, Pseudomonas syringae pv syringaee0
- Interactor
- Pseudomonas syringae pv syringae
Pseudomonas syringaee0pathogen - Outcome
- expression of the hypersensitive reaction and in phytoalexin accumulatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Inoculation of leaves of Arabidopsis thaliana (L.) Heynh. with the wheat pathogen, Pseudomonas syringae pv syringae, resulted in the expression of the hypersensitive reaction and in phytoalexin accumulation.”
Claim · model-written summary
The Pseudomonas mutant did not elicit detectable phytoalexin accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- infiltration of leaves with a mutant of P. s. syringaee0
- Interactor
- P. s. syringaee0pathogen
- Outcome
- No phytoalexin accumulatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No phytoalexin accumulation was detected after infiltration of leaves with a mutant of P. s. syringae deficient in the ability to elicit a hypersensitive reaction”
t0“Arabidopsis thaliana”
Claim · model-written summary
Xanthomonas infiltration did not elicit detectable phytoalexin accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- Xanthomonas campestris pv campestrise0
- Interactor
- Xanthomonas campestris pv campestris
_(25868012152).jpg?width=60)
Xanthomonas campestrise0pathogen - Outcome
- No phytoalexin accumulatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(25868012152).jpg?width=500)
Verbatim
e0“No phytoalexin accumulation was detected after infiltration of leaves with a mutant of P. s. syringae deficient in the ability to elicit a hypersensitive reaction; with the crucifer pathogen, Xanthomonas campestris pv campestris”
t0“Arabidopsis thaliana”
Claim · model-written summary
Potassium phosphate buffer infiltration did not elicit detectable phytoalexin accumulation.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- 10 millimolar potassium phosphate buffer (pH 6.9)e0
- Outcome
- No phytoalexin accumulatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“No phytoalexin accumulation was detected after infiltration of leaves with a mutant of P. s. syringae deficient in the ability to elicit a hypersensitive reaction; with the crucifer pathogen, Xanthomonas campestris pv campestris; or with 10 millimolar potassium phosphate buffer (pH 6.9).”
t0“Arabidopsis thaliana”
Claim · model-written summary
Phytoalexin accumulation correlated with restricted Pseudomonas growth.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Interactor
- P. s. syringaee0pathogen
- Outcome
- restricted in vivo growth of P. s. syringaee0
- Direction
- restrictede0
- Qualifier
- Phytoalexin accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phytoalexin accumulation was correlated with the restricted in vivo growth of P. s. syringae.”
t0“Arabidopsis thaliana”
Claim · model-written summary
The Arabidopsis phytoalexin was identified as 3-thiazol-2'-yl-indole.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- 3-thiazol-2'-yl-indolee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Arabidopsis phytoalexin was identified as 3-thiazol-2'-yl-indole”
t0“Arabidopsis thaliana”
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