Abstract
Protons (H+) in acidic soils arrest plant growth. However, the mechanisms by which plants optimize their biological processes to diminish the unfavorable effects of H+ stress remain largely unclear. Here, we showed that in the roots of Arabidopsis thaliana, the C2H2-type transcription factor STOP1 in the nucleus was enriched by low pH in a nitrate-independent manner, with the spatial expression pattern of NITRATE TRANSPORTER 1.1 (NRT1.1) established by low pH required the action of STOP1. Additionally, the nrt1.1 and stop1 mutants, as well as the nrt1.1 stop1 double mutant, had a similar hypersensitive phenotype to low pH, indicating that STOP1 and NRT1.1 function in the same pathway for H+ tolerance. Molecular assays revealed that STOP1 directly bound to the promoter of NRT1.1 to activate its transcription in response to low pH, thus upregulating its nitrate uptake. This action improved the nitrogen use efficiency (NUE) of plants and created a favorable rhizospheric pH for root growth by enhancing H+ depletion in the rhizosphere. Consequently, the constitutive expression of NRT1.1 in stop1 mutants abolished the hypersensitive phenotype to low pH. These results demonstrate that STOP1-NRT1.1 is a key module for plants to optimize NUE and ensure better plant growth in acidic media.
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Claims 6
Claim · model-written summary
Low pH enriched nuclear STOP1 in Arabidopsis roots independently of nitrate.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Organ / stage
- rootse0
- Treatment
- low pHe0
- Gene
- STOP1e0
- Outcome
- STOP1 in the nucleuse0
- Direction
- enrichede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“in the roots of Arabidopsis thaliana, the C2H2-type transcription factor STOP1 in the nucleus was enriched by low pH in a nitrate-independent manner”
Claim · model-written summary
STOP1 was required for the low pH spatial expression pattern of NRT1.1.
- Gene
- NITRATE TRANSPORTER 1.1 (NRT1.1)e0
- Outcome
- spatial expression patterne0
- Qualifier
- required the action of STOP1e0
Not reportedTaxon
Verbatim
e0“the spatial expression pattern of NITRATE TRANSPORTER 1.1 (NRT1.1) established by low pH required the action of STOP1.”
Claim · model-written summary
nrt1.1, stop1, and double mutants showed similar low pH hypersensitivity.
Not reportedTaxon
Verbatim
e0“the nrt1.1 and stop1 mutants, as well as the nrt1.1 stop1 double mutant, had a similar hypersensitive phenotype to low pH”
Claim · model-written summary
STOP1 bound the NRT1.1 promoter and activated its transcription under low pH.
- Gene
- NRT1.1e0
- Outcome
- transcriptione0
- Direction
- activatee0
- Qualifier
- STOP1 directly bound to the promotere0
Not reportedTaxon
Verbatim
e0“STOP1 directly bound to the promoter of NRT1.1 to activate its transcription in response to low pH”
Claim · model-written summary
STOP1 upregulated nitrate uptake, improving nitrogen use efficiency and rhizosphere pH for root growth.
- Taxon
- plantsViridiplantaee0
- Outcome
- nitrogen use efficiency (NUE)e0
- Direction
- improvede0
- Qualifier
- by enhancing H+ depletion in the rhizospheree0
Verbatim
e0“thus upregulating its nitrate uptake. This action improved the nitrogen use efficiency (NUE) of plants and created a favorable rhizospheric pH for root growth by enhancing H+ depletion in the rhizosphere.”
Claim · model-written summary
Constitutive NRT1.1 expression abolished low pH hypersensitivity in stop1 mutants.
- Material
- stop1 mutantse0
- Treatment
- constitutive expression of NRT1.1e0
- Gene
- NRT1.1e0
- Outcome
- hypersensitive phenotype to low pHe0
- Direction
- abolishede0
Not reportedTaxonComparator
Verbatim
e0“the constitutive expression of NRT1.1 in stop1 mutants abolished the hypersensitive phenotype to low pH.”
The same questions in other papers
ph · molecular: metabolites · Arabidopsis thaliana (thale cress)Positive and negative results
3 in the opposite direction
- negative
- negative
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.