Abstract
This work was conducted to evaluate the effect of N source (NH4+/NO3-) and the implication of NH4+-induced acidification on plant development in Arabidopsis thaliana. seedlings were hydroponically cultivated during 21 days under three ratios of NO3−/NH4+ (mM); 2.5/0.0; 1.25/1.25, and 0.0/2.5. For each ratio, plants were cultivated under not controlled pH and adjusted pH at 6.2. Ammonium-fed plants (0.0/2.5) showed chlorosis if cultivated under uncontrolled pH. This symptom was associated with suppressions in plant growth, chlorophyll and iron contents as well as shoots hydration and nutrient concentration. Growth was limited with increasing NH4+ medium-concentration. At pH 6.2, plants receiving only NH4+ produced the same biomass as NO3-fed ones. The mineral disturbances in plants grown on strictly NH4+-medium (0.0/2.5) were alleviated under controlled pH. The activities of nitrate reductase (NR) and nitrite reductase (NIR) were lower in plants amended only with NH4+ under uncontrolled pH. At adjusted pH, the NR activity was ameliorated but that of NIR was not ameliorated, suggesting hence that NR activity depended on medium pH but that of NIR was related only to the pool of NO2-. In conclusion, this work demonstrated that the adjustment of pH at 6.2 mitigated significantly the toxic effects of ammonium to A. thaliana.
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Claims 8
Claim · model-written summary
Ammonium-fed plants under uncontrolled pH showed chlorosis.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- Ammonium-fed plants (0.0/2.5)e0
- Outcome
- chlorosise0
- Qualifier
- uncontrolled pHe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammonium-fed plants (0.0/2.5) showed chlorosis if cultivated under uncontrolled pH.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Chlorosis was associated with suppressed growth, chlorophyll and iron contents, shoot hydration and nutrient concentration.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- NH 4 + -induced toxicityt0
- Outcome
- plant growth, chlorophyll and iron contents as well as shoots hydration and nutrient concentratione0
- Direction
- suppressionse0
- Qualifier
- This symptome0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This symptom was associated with suppressions in plant growth, chlorophyll and iron contents as well as shoots hydration and nutrient concentration.”
t0“NH 4 + -induced toxicity in Arabidopsis thaliana”
Claim · model-written summary
Growth fell as ammonium concentration increased.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- increasing NH4+ medium-concentratione0
- Outcome
- Growthe0
- Direction
- limitede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Growth was limited with increasing NH4+ medium-concentration.”
t0“Arabidopsis thaliana”
Claim · model-written summary
At pH 6.2, ammonium-only plants had biomass equal to nitrate-fed plants.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- only NH4+e0
- Outcome
- biomasse0
- Direction
- samee0
- Comparator
- NO3-fed onese0
- Setting
- pH 6.2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At pH 6.2, plants receiving only NH4+ produced the same biomass as NO3-fed ones.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Controlled pH alleviated mineral disturbances under ammonium-only medium.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- controlled pHe0
- Outcome
- mineral disturbancese0
- Direction
- alleviatede0
- Qualifier
- strictly NH4+-medium (0.0/2.5)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The mineral disturbances in plants grown on strictly NH4+-medium (0.0/2.5) were alleviated under controlled pH.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Nitrate reductase and nitrite reductase activities were lower with ammonium alone at uncontrolled pH.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- only with NH4+e0
- Outcome
- activities of nitrate reductase (NR) and nitrite reductase (NIR)e0
- Direction
- lowere0
- Qualifier
- uncontrolled pHe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activities of nitrate reductase (NR) and nitrite reductase (NIR) were lower in plants amended only with NH4+ under uncontrolled pH.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Adjusted pH improved nitrate reductase activity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- adjusted pHe0
- Outcome
- NR activitye0
- Direction
- amelioratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At adjusted pH, the NR activity was ameliorated but that of NIR was not ameliorated, suggesting hence that NR activity depended on medium pH but that of NIR was related only to the pool of NO2-.”
t0“Arabidopsis thaliana”
Claim · model-written summary
Adjusted pH did not improve nitrite reductase activity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- adjusted pHe0
- Outcome
- NIRe0
- Direction
- not amelioratede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“At adjusted pH, the NR activity was ameliorated but that of NIR was not ameliorated, suggesting hence that NR activity depended on medium pH but that of NIR was related only to the pool of NO2-.”
t0“Arabidopsis thaliana”
The same questions in other papers
ammonium · growth · Arabidopsis thaliana (thale cress)Results agree
3 papers in the same direction as this paper
- negative
- negative
- negative
ph · molecular: metabolites · Arabidopsis thaliana (thale cress)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- positive
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.