Abstract
When hypocotyl segments of the spinach plant were cultured on medium containing IAA (15mg/l) in the presence of GA, those cultivars in which the callus growth was profuse in terms of the concentrations of GA (10-7-10-4M), e.g. ‘HOYO’, ‘TOKAI’ and ‘WAKAKUSA’, showed generally low bud-forming activity. Furthermore, in the cultivars which had relatively high bud-forming capacity on the medium containing IAA plus GA, e.g. ‘NIPPON’ and ‘TOKO’, callus proliferation of their hypocotyl tissue segments were low overall. Root formation derived from hypocotyl segments of ‘TOKO’ and ‘NIPPON’ were markedly enhanced by the simultaneous application of GA to the medium with IAA. While the effective concentrations of GA on bud formation of spinach hypocotyl segments were different among these cultivars, the bud formation was enhanced by the addition of GA at the concentrations ranging from 10-7 to 10-4M, except in the case of ‘TOKAI’. Optimal concentrations of GA on the bud formation of ‘NIPPON’, ‘TOKO’ and ‘HOMO’ were 10-5, 10-6 and 10-4M, respectively. The induction of bud formation of hypocotyl segments in the spinach plant may take place during a relatively early stage of the incubation.
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Claims 8
Claim · model-written summary
Cultivars with profuse GA-associated callus growth generally had low bud-forming activity.
- Taxon
- Spinach
Spinacia oleraceat0 - Material
- ‘HOYO’, ‘TOKAI’ and ‘WAKAKUSA’e0
- Treatment
- GA (10-7-10-4M)e0
- Outcome
- bud-forming activitye0
- Direction
- lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those cultivars in which the callus growth was profuse in terms of the concentrations of GA (10-7-10-4M), e.g. ‘HOYO’, ‘TOKAI’ and ‘WAKAKUSA’, showed generally low bud-forming activity.”
t0“Spinach”
Claim · model-written summary
NIPPON and TOKO had high bud-forming capacity but low callus proliferation.
- Taxon
- SpinachSpinacia oleraceat0
- Material
- ‘NIPPON’ and ‘TOKO’e0
- Treatment
- IAA plus GAe1
- Outcome
- callus proliferatione0
- Direction
- lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“‘NIPPON’ and ‘TOKO’, callus proliferation of their hypocotyl tissue segments were low overall.”
e1“cultivars which had relatively high bud-forming capacity on the medium containing IAA plus GA”
t0“Spinach”
Claim · model-written summary
GA added with IAA markedly enhanced root formation in TOKO and NIPPON.
- Taxon
- SpinachSpinacia oleraceat0
- Material
- ‘TOKO’ and ‘NIPPON’e0
- Organ / stage
- hypocotyl segmentse0
- Treatment
- simultaneous application of GA to the medium with IAAe0
- Outcome
- Root formatione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Root formation derived from hypocotyl segments of ‘TOKO’ and ‘NIPPON’ were markedly enhanced by the simultaneous application of GA to the medium with IAA.”
t0“Spinach”
Claim · model-written summary
GA enhanced bud formation except in TOKAI across the reported concentrations.
- Taxon
- SpinachSpinacia oleraceat0
- Treatment
- addition of GA at the concentrations ranging from 10-7 to 10-4Me0
- Outcome
- bud formatione0
- Direction
- enhancede0
- Qualifier
- except in the case of ‘TOKAI’e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“bud formation was enhanced by the addition of GA at the concentrations ranging from 10-7 to 10-4M, except in the case of ‘TOKAI’.”
t0“Spinach”
Claim · model-written summary
The optimal GA concentration for bud formation in NIPPON was 10-5.
- Taxon
- SpinachSpinacia oleraceat0
- Material
- ‘NIPPON’e0
- Outcome
- bud formatione0
- Value
- 10-5e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal concentrations of GA on the bud formation of ‘NIPPON’, ‘TOKO’ and ‘HOMO’ were 10-5, 10-6 and 10-4M, respectively.”
t0“Spinach”
Claim · model-written summary
The optimal GA concentration for bud formation in TOKO was 10-6.
- Taxon
- SpinachSpinacia oleraceat0
- Material
- ‘TOKO’e0
- Outcome
- bud formatione0
- Value
- 10-6e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal concentrations of GA on the bud formation of ‘NIPPON’, ‘TOKO’ and ‘HOMO’ were 10-5, 10-6 and 10-4M, respectively.”
t0“Spinach”
Claim · model-written summary
The optimal GA concentration for bud formation in HOMO was 10-4M.
- Taxon
- SpinachSpinacia oleraceat0
- Material
- ‘HOMO’e0
- Outcome
- bud formatione0
- Value
- 10-4Me0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Optimal concentrations of GA on the bud formation of ‘NIPPON’, ‘TOKO’ and ‘HOMO’ were 10-5, 10-6 and 10-4M, respectively.”
t0“Spinach”
Claim · model-written summary
Bud induction may occur early in incubation.
- Taxon
- spinachSpinacia oleraceae0
- Outcome
- induction of bud formatione0
- Qualifier
- relatively early stage of the incubatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The induction of bud formation of hypocotyl segments in the spinach plant may take place during a relatively early stage of the incubation.”
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