Abstract
As both major macronutrients and signal molecules, nitrogen metabolites, such as nitrate and nitrite, play an important role in plant growth and development. In this study, the callus growth of indica rice cv. 9311 was significantly enhanced by nitrite, whereas the soluble protein content remained unchanged. The deep RNA sequencing technology (RNA-seq) showed that the transcriptional profiles of cv. 9311 calli were significantly changed after adding nitrite to the nitrate-free medium, and these nitrite-responsive genes were involved in a wide range of plant processes, particularly in the secondary metabolite pathways. Interestingly, most of the genes involved in phenylpropanoid-related pathways were coordinately down-regulated by nitrite, such as four cinnamoyl-CoA reductase, and these in turn resulted in the decrease of lignin content of indica calli. Furthermore, several candidate genes related to cell growth or stress responses were identified, such as genes coding for expansins, SMALL AUXIN UP RNA (SAUR) and HSP20s, and these suggested that nitrite could probably serve as a transcriptome signal to enhance the indica calli growth by regulation of various downstream genes expression. This study contributes to a better understanding of the function of nitrite during the process of plant tissue culture and could aid in the application of this technology to improved indica genetic transformation efficiency.
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Claims 7
Claim · model-written summary
Nitrite significantly enhanced indica rice callus growth.
- Taxon
- indica rice
Oryza sativa Indica Groupe0 - Material
- cv. 9311e0
- Organ / stage
- calluse0
- Treatment
- nitritee0
- Outcome
- callus growthe0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the callus growth of indica rice cv. 9311 was significantly enhanced by nitrite”
Claim · model-written summary
Soluble protein content remained unchanged under nitrite.
Not reportedTaxon
Verbatim
e0“whereas the soluble protein content remained unchanged.”
Claim · model-written summary
Nitrite significantly changed transcription profiles of rice calli.
- Material
- cv. 9311e0
- Organ / stage
- callie0
- Treatment
- adding nitrite to the nitrate-free mediume0
- Outcome
- transcriptional profilese0
- Direction
- changede0
Not reportedTaxonComparator
Verbatim
e0“the transcriptional profiles of cv. 9311 calli were significantly changed after adding nitrite to the nitrate-free medium”
Claim · model-written summary
Nitrite coordinately downregulated phenylpropanoid pathway genes including cinnamoyl-CoA reductase.
- Treatment
- nitritee0
- Gene
- cinnamoyl-CoA reductasee0
- Outcome
- genes involved in phenylpropanoid-related pathwayse0
- Direction
- down-regulatede0
Not reportedTaxonComparator
Verbatim
e0“most of the genes involved in phenylpropanoid-related pathways were coordinately down-regulated by nitrite, such as four cinnamoyl-CoA reductase”
Claim · model-written summary
Nitrite decreased lignin content of indica rice calli.
Not reportedTreatmentComparator
Verbatim
e0“these in turn resulted in the decrease of lignin content of indica calli.”
Claim · model-written summary
The study identified candidate growth and stress-response genes including expansins, SAUR and HSP20s.
- Gene
- expansins, SMALL AUXIN UP RNA (SAUR) and HSP20se0
- Outcome
- candidate genes related to cell growth or stress responsese0
Not reportedTaxon
Verbatim
e0“several candidate genes related to cell growth or stress responses were identified, such as genes coding for expansins, SMALL AUXIN UP RNA (SAUR) and HSP20s”
Claim · model-written summary
The authors suggest nitrite may stimulate callus growth by regulating downstream gene expression.
Verbatim
e0“nitrite could probably serve as a transcriptome signal to enhance the indica calli growth by regulation of various downstream genes expression.”
The same questions in other papers
nitrite · growth · Oryza sativa (rice)Positive and negative results
1 in the opposite direction
- no effect
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.