Abstract
Loading of cytosolic nitrite into the chloroplast in nitrate assimilation of rice was modified by introducing a gene for chloroplastic nitrite transport from cucumber. Rice (Oryza sativa L. japonica cv. Nipponbare) was transformed with a cDNA (CsNitr1-L) that encodes a nitrite transporter of cucumber under the control of the CaMV35S promoter. CsNitr1-L transgenic rice grown by hydroponics with nitrate supplied as the sole nitrogen source grew as well as plants grown with ammonia. In contrast the growth of the untransformed or mock-transformed control rice was retarded on a nitrate-containing medium by about 60% compared to plants grown with ammonia. Nitrite was not beneficial to the growth of the untransformed or mock-transformed control rice but sustained that of CsNitr1-L transgenic rice. Low steady-state concentration of nitrite in leaves as well as delayed non-photochemical quenching of chlorophyll fluorescence in CsNitr1-L transgenic rice seems indicative of higher nitrite use in chloroplasts.
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Claims 7
Claim · model-written summary
CsNitr1-L transgenic rice grown with nitrate grew as well as plants grown with ammonia.
- Taxon
- rice
Oryza sativae0 - Material
- CsNitr1-L transgenic ricee0
- Treatment
- nitrate supplied as the sole nitrogen sourcee0
- Gene
- CsNitr1-Le0
- Outcome
- grewe0
- Direction
- as well ase0
- Comparator
- plants grown with ammoniae0
- Setting
- hydroponicse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“CsNitr1-L transgenic rice grown by hydroponics with nitrate supplied as the sole nitrogen source grew as well as plants grown with ammonia.”
Claim · model-written summary
Control rice growth on nitrate was retarded by about 60% relative to ammonia.
- Taxon
- riceOryza sativae0
- Material
- untransformed or mock-transformed control ricee0
- Treatment
- nitrate-containing mediume0
- Outcome
- growthe0
- Direction
- retardede0
- Value
- about 60%e0
- Comparator
- plants grown with ammoniae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the growth of the untransformed or mock-transformed control rice was retarded on a nitrate-containing medium by about 60% compared to plants grown with ammonia.”
Claim · model-written summary
Nitrite did not benefit growth of untransformed or mock-transformed rice.
- Taxon
- RiceOryza sativae1
- Material
- untransformed or mock-transformed control ricee0
- Treatment
- Nitritee0
- Outcome
- growthe0
- Direction
- not beneficiale0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Nitrite was not beneficial to the growth of the untransformed or mock-transformed control rice”
e1“Rice (Oryza sativa L. japonica cv. Nipponbare)”
Claim · model-written summary
Nitrite sustained growth of CsNitr1-L transgenic rice.
- Taxon
- riceOryza sativae0
- Material
- CsNitr1-L transgenic ricee0
- Treatment
- Nitritee1
- Gene
- CsNitr1-Le0
- Outcome
- sustainede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“but sustained that of CsNitr1-L transgenic rice”
e1“Nitrite was not beneficial to the growth of the untransformed or mock-transformed control rice”
Claim · model-written summary
Transgenic rice leaves had low steady-state nitrite concentration.
- Taxon
- riceOryza sativae0
- Material
- CsNitr1-L transgenic ricee0
- Organ / stage
- leavese0
- Outcome
- Low steady-state concentration of nitritee0
- Direction
- Lowe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low steady-state concentration of nitrite in leaves as well as delayed non-photochemical quenching of chlorophyll fluorescence in CsNitr1-L transgenic rice”
e1“Rice (Oryza sativa L. japonica cv. Nipponbare)”
Claim · model-written summary
CsNitr1-L rice showed delayed non-photochemical quenching of chlorophyll fluorescence.
- Taxon
- riceOryza sativae0
- Material
- CsNitr1-L transgenic ricee0
- Outcome
- non-photochemical quenching of chlorophyll fluorescencee0
- Direction
- delayede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“delayed non-photochemical quenching of chlorophyll fluorescence in CsNitr1-L transgenic rice”
Claim · model-written summary
The leaf nitrite and fluorescence findings were interpreted as higher chloroplast nitrite use.
- Taxon
- riceOryza sativae0
- Material
- CsNitr1-L transgenic ricee0
- Outcome
- higher nitrite use in chloroplastse0
- Qualifier
- seems indicativee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Low steady-state concentration of nitrite in leaves as well as delayed non-photochemical quenching of chlorophyll fluorescence in CsNitr1-L transgenic rice seems indicative of higher nitrite use in chloroplasts.”
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See all 511 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.