Abstract
The objective of this study was to identify barley leaf proteins differentially regulated in response to drought and heat and the combined stresses in context of the morphological and physiological changes that also occur. The Syrian landrace Arta and the Australian cultivar Keel were subjected to drought, high temperature, or a combination of both treatments starting at heading. Changes in the leaf proteome were identified using differential gel electrophoresis and mass spectrometry. The drought treatment caused strong reductions of biomass and yield, while photosynthetic performance and the proteome were not significantly changed. In contrast, the heat treatment and the combination of heat and drought reduced photosynthetic performance and caused changes of the leaf proteome. The proteomic analysis identified 99 protein spots differentially regulated in response to heat treatment, 14 of which were regulated in a genotype-specific manner. Differentially regulated proteins predominantly had functions in photosynthesis, but also in detoxification, energy metabolism, and protein biosynthesis. The analysis indicated that de novo protein biosynthesis, protein quality control mediated by chaperones and proteases, and the use of alternative energy resources, i.e. glycolysis, play important roles in adaptation to heat stress. In addition, genetic variation identified in the proteome, in plant growth and photosynthetic performance in response to drought and heat represent stress adaption mechanisms to be exploited in future crop breeding efforts.
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Claims 11
Claim · model-written summary
Drought strongly reduced barley biomass and yield.
- Taxon
- barley
.jpg?width=60)
Hordeum vulgaret0 - Treatment
- drought treatmente0
- Outcome
- biomass and yielde0
- Direction
- strong reductionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The drought treatment caused strong reductions of biomass and yield”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Drought did not significantly change barley photosynthetic performance or the leaf proteome.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- photosynthetic performance and the proteomee0
- Direction
- not significantly changede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“photosynthetic performance and the proteome were not significantly changed”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Heat reduced barley photosynthetic performance.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- heat treatmente0
- Outcome
- photosynthetic performancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heat treatment and the combination of heat and drought reduced photosynthetic performance”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Combined heat and drought reduced barley photosynthetic performance.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- combination of heat and droughte0
- Outcome
- photosynthetic performancee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the combination of heat and drought reduced photosynthetic performance”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Heat changed the barley leaf proteome.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- heat treatmente0
- Outcome
- leaf proteomee0
- Direction
- changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the heat treatment and the combination of heat and drought reduced photosynthetic performance and caused changes of the leaf proteome”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Combined heat and drought changed the barley leaf proteome.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- combination of heat and droughte0
- Outcome
- leaf proteomee0
- Direction
- changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the combination of heat and drought reduced photosynthetic performance and caused changes of the leaf proteome”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Heat differentially regulated 99 barley protein spots.
- Taxon
- barleyHordeum vulgaret0
- Treatment
- heat treatmente0
- Outcome
- protein spots differentially regulatede0
- Value
- 99 protein spotse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“identified 99 protein spots differentially regulated in response to heat treatment”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Fourteen heat-responsive barley protein spots were regulated in a genotype-specific manner.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- regulated in a genotype-specific mannere0
- Value
- 14e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“14 of which were regulated in a genotype-specific manner”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Differentially regulated barley proteins mainly functioned in photosynthesis, with others in detoxification, energy metabolism and protein biosynthesis.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- functions in photosynthesis, but also in detoxification, energy metabolism, and protein biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Differentially regulated proteins predominantly had functions in photosynthesis, but also in detoxification, energy metabolism, and protein biosynthesis”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
The analysis indicated that protein synthesis, quality control and glycolysis contribute to barley heat adaptation.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- adaptation to heat stresse0
- Qualifier
- de novo protein biosynthesis, protein quality control mediated by chaperones and proteases, and the use of alternative energy resources, i.e. glycolysise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“de novo protein biosynthesis, protein quality control mediated by chaperones and proteases, and the use of alternative energy resources, i.e. glycolysis, play important roles in adaptation to heat stress”
t0“barley (Hordeum vulgare L.)”
Claim · model-written summary
Genetic variation in barley proteome, growth and photosynthesis under stress was identified as potentially useful for breeding.
- Taxon
- barleyHordeum vulgaret0
- Outcome
- genetic variatione0
- Qualifier
- in response to drought and heate0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“genetic variation identified in the proteome, in plant growth and photosynthetic performance in response to drought and heat represent stress adaption mechanisms to be exploited in future crop breeding efforts”
t0“barley (Hordeum vulgare L.)”
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See all 102 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.