Abstract
Amongst various antioxidant enzymes, superoxide dismutase (SOD) has been found to play most important role in the tolerance mechanism of the plants to the abiotic stresses. In this study, full length cDNA of 733 bp encoding superoxide dismutase, designated as TaSOD, was isolated and characterized from wheat (Triticum aestivum). A manganese iron-binding domain was observed in the sequence using CD search tool of NCBI. Mapping of wheat genome by southern blotting showed the presence of three copies of TaSOD gene. Quantitative transcript profiling of superoxide dismutase showed 1.3, 1.8, 1.9 fold increase in thermotolerant genotype (HDR77), 1.1, 1.3, 1.5 fold increase in thermosusceptible (PBW343) at pollination, milky dough and seed hardening stages respectively in leaves because of diurnal changes in environmental temperature. Abundance of TaSOD transcript and high SOD activity, total antioxidant capacity and cell membrane stability index were observed in HDR77 in response to the exogenous application of H2O2 (10 mmole L -1 ) compared to that of salicylic acid (5 mmole L -1 ) under differential heat shock. Isoenzymes analysis of SOD showed expression of three prominent isoenzymes in HDR77 and only two in PBW343 under the heat stress, with maximum activity at pollination and seed-hardening stages in both the cultivars. Thermotolerance capacity was analyzed using cell membrane stability and total antioxidant capacity; both the parameters were high in thermotolerant (HDR77) where abundance of SOD transcript was also observed as compared to thermosusceptible (PBW343). Superoxide dismutase regulates the expression of various heat stress associated genes through balancing the accumulation of H2O2 inside cells, which also act as signaling molecules. However, there is a greater need to further characterize SODs and over-expressed in the desirable crop-cultivars with a view to enhance the tolerance capacity against various abiotic stresses.
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Claims 16
Claim · model-written summary
A 733 bp full length TaSOD cDNA was isolated from wheat.
- Taxon
- wheat (Triticum aestivum)
Triticum aestivume0 - Gene
- TaSODe0
- Outcome
- full length cDNAe0
- Value
- 733 bpe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“full length cDNA of 733 bp encoding superoxide dismutase, designated as TaSOD, was isolated and characterized from wheat (Triticum aestivum).”
Claim · model-written summary
The TaSOD sequence has a manganese iron-binding domain.
- Taxon
- wheatTriticum aestivumt0
- Gene
- mtSODt0
- Outcome
- manganese iron-binding domaine0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A manganese iron-binding domain was observed in the sequence”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
Three copies of TaSOD occur in the wheat genome.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaSODe0
- Outcome
- copies of TaSOD genee0
- Value
- three copiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Mapping of wheat genome by southern blotting showed the presence of three copies of TaSOD gene.”
Claim · model-written summary
HDR77 TaSOD transcripts increased 1.3 fold at pollination with diurnal temperature change.
- Taxon
- wheatTriticum aestivumt0
- Material
- HDR77e0
- Gene
- superoxide dismutasee0
- Outcome
- transcripte0
- Direction
- increasee0
- Value
- 1.3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative transcript profiling of superoxide dismutase showed 1.3, 1.8, 1.9 fold increase in thermotolerant genotype (HDR77)”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
HDR77 TaSOD transcripts increased 1.8 fold at milky dough.
- Taxon
- wheatTriticum aestivumt0
- Material
- HDR77e0
- Gene
- superoxide dismutasee0
- Outcome
- transcripte0
- Direction
- increasee0
- Value
- 1.8e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative transcript profiling of superoxide dismutase showed 1.3, 1.8, 1.9 fold increase in thermotolerant genotype (HDR77)”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
HDR77 TaSOD transcripts increased 1.9 fold at seed hardening.
- Taxon
- wheatTriticum aestivumt0
- Material
- HDR77e0
- Gene
- superoxide dismutasee0
- Outcome
- transcripte0
- Direction
- increasee0
- Value
- 1.9e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative transcript profiling of superoxide dismutase showed 1.3, 1.8, 1.9 fold increase in thermotolerant genotype (HDR77)”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
PBW343 transcripts increased 1.1 fold at pollination.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative transcript profiling of superoxide dismutase showed 1.3, 1.8, 1.9 fold increase in thermotolerant genotype (HDR77), 1.1, 1.3, 1.5 fold increase in thermosusceptible (PBW343)”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
PBW343 transcripts increased 1.3 fold at milky dough.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative transcript profiling of superoxide dismutase showed 1.3, 1.8, 1.9 fold increase in thermotolerant genotype (HDR77), 1.1, 1.3, 1.5 fold increase in thermosusceptible (PBW343)”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
PBW343 transcripts increased 1.5 fold at seed hardening.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative transcript profiling of superoxide dismutase showed 1.3, 1.8, 1.9 fold increase in thermotolerant genotype (HDR77), 1.1, 1.3, 1.5 fold increase in thermosusceptible (PBW343)”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
HDR77 had greater TaSOD transcript abundance, SOD activity, antioxidant capacity and membrane stability after H2O2 than salicylic acid under heat shock.
- Taxon
- wheatTriticum aestivumt0
- Material
- HDR77e0
- Treatment
- H2O2 (10 mmole L -1 )e0
- Gene
- TaSODe0
- Outcome
- TaSOD transcript and high SOD activity, total antioxidant capacity and cell membrane stability indexe0
- Direction
- highe0
- Qualifier
- differential heat shocke0
- Comparator
- salicylic acid (5 mmole L -1 )e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Abundance of TaSOD transcript and high SOD activity, total antioxidant capacity and cell membrane stability index were observed in HDR77 in response to the exogenous application of H2O2 (10 mmole L -1 ) compared to that of salicylic acid (5 mmole L -1 ) under differential heat shock.”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
Three prominent SOD isoenzymes were expressed in HDR77 under heat stress.
- Taxon
- wheatTriticum aestivumt0
- Material
- HDR77e0
- Gene
- SODt0
- Outcome
- isoenzymese0
- Value
- threee0
- Qualifier
- heat stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three prominent isoenzymes in HDR77 and only two in PBW343 under the heat stress”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
Two SOD isoenzymes were expressed in PBW343 under heat stress.
- Taxon
- wheatTriticum aestivumt0
- Material
- PBW343e0
- Gene
- SODt0
- Outcome
- isoenzymese0
- Value
- twoe0
- Qualifier
- heat stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“three prominent isoenzymes in HDR77 and only two in PBW343 under the heat stress”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
SOD isoenzyme activity was maximal at pollination and seed hardening in both cultivars.
- Taxon
- wheatTriticum aestivumt0
- Organ / stage
- pollination and seed-hardening stagese0
- Outcome
- maximum activitye0
- Direction
- maximume0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“with maximum activity at pollination and seed-hardening stages in both the cultivars.”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
HDR77 had higher membrane stability and total antioxidant capacity than PBW343.
- Taxon
- wheatTriticum aestivumt0
- Material
- HDR77e0
- Outcome
- both the parameterse0
- Direction
- highe0
- Comparator
- PBW343e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“both the parameters were high in thermotolerant (HDR77) where abundance of SOD transcript was also observed as compared to thermosusceptible (PBW343).”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
The authors assert that superoxide dismutase regulates heat stress gene expression by balancing cellular H2O2.
- Taxon
- wheatTriticum aestivumt0
- Gene
- Superoxide dismutasee0
- Compound
- H2O2e0
- Outcome
- expression of various heat stress associated genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Superoxide dismutase regulates the expression of various heat stress associated genes through balancing the accumulation of H2O2 inside cells”
t0“mtSOD) in wheat (Triticum aestivum)”
Claim · model-written summary
The authors recommend further SOD characterization and over-expression in crop cultivars to improve abiotic stress tolerance.
- Taxon
- wheatTriticum aestivumt0
- Gene
- SODse0
- Outcome
- further characterize SODs and over-expressed in the desirable crop-cultivarse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there is a greater need to further characterize SODs and over-expressed in the desirable crop-cultivars with a view to enhance the tolerance capacity against various abiotic stresses.”
t0“mtSOD) in wheat (Triticum aestivum)”
The same questions in other papers
hydrogen peroxide · physiology: antioxidant enzymes · Triticum aestivum (wheat)Results differ
13 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
12 papers more in this group
See all 16 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.