Paper · 2006 · Plant Biotechnology

Nuclear localization of sweet cherry DREB1/CBF ortholog (CIG) and low temperature-inducible activity of CIG promoter

Hiroyasu Kitashiba; Yusuke Ban; Chikako Honda; et al.

4
claims
7
verbatim quotes
2
citations
3
research areas
✓
Open access

Abstract

The fluorescent signal of GFP fused to CIG-B, which is one of the three DREB1/CBF (dehydration responsive element binding protein 1/C-repeat binding factor) homologs isolated from sweet cherry, was observed only in nucleus. The expression of GFP driven by CIG-B promoter was up-regulated by low-temperature. These results suggested that nuclear localization signal (NLS) motif in the N-terminal region of CIG-B protein and inducer of CBF expression (ICE)-like motifs in CIG-B promoter could be responsible for nuclear targeting and low temperature perception, respectively.

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Claims 4

Observationobservedoriginal resultmeasured

Claim · model-written summary

The CIG-B GFP signal was observed only in the nucleus.

Taxon
sweet cherryPrunus aviume0
Organ / stage
nucleuse0
Gene
CIG-Be0
Outcome
fluorescent signal of GFP fused to CIG-Be0

Not reportedDirection

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus avium
Prunus avium · TaxonMPF · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The fluorescent signal of GFP fused to CIG-B, which is one of the three DREB1/CBF (dehydration responsive element binding protein 1/C-repeat binding factor) homologs isolated from sweet cherry, was observed only in nucleus”

✓ located verbatim in the source · abstract, characters 0–222 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Low temperature up-regulated expression driven by the CIG-B promoter.

Taxon
sweet cherryPrunus aviumt0
Treatment
low-temperaturee0
Gene
CIG-Be0
Outcome
expression of GFP driven by CIG-B promotere0
Direction
up-regulatede0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus avium
Prunus avium · TaxonMPF · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“The expression of GFP driven by CIG-B promoter was up-regulated by low-temperature”

✓ located verbatim in the source · abstract, characters 224–306 · show in text ↑
t0

“sweet cherry”

✓ located verbatim in the source · title, characters 24–36 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors suggest that the NLS motif in the CIG-B N-terminal region could mediate nuclear targeting.

Taxon
sweet cherryPrunus aviumt0
Gene
CIG-Be0
Outcome
nuclear targetinge0
Qualifier
nuclear localization signal (NLS) motif in the N-terminal regione0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus avium
Prunus avium · TaxonMPF · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“nuclear localization signal (NLS) motif in the N-terminal region of CIG-B protein and inducer of CBF expression (ICE)-like motifs in CIG-B promoter could be responsible for nuclear targeting and low temperature perception, respectively”

✓ located verbatim in the source · abstract, characters 337–572 · show in text ↑
t0

“sweet cherry”

✓ located verbatim in the source · title, characters 24–36 · show in text ↑
Mechanismauthor interpretationoriginal resultmeasured

Claim · model-written summary

The authors suggest that ICE-like motifs in the CIG-B promoter could mediate low temperature perception.

Taxon
sweet cherryPrunus aviumt0
Gene
CIG-Be0
Outcome
low temperature perceptione0
Qualifier
inducer of CBF expression (ICE)-like motifse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Prunus avium
Prunus avium · TaxonMPF · CC BY 2.5 · Wikimedia Commons ↗

Verbatim

e0

“inducer of CBF expression (ICE)-like motifs in CIG-B promoter could be responsible for nuclear targeting and low temperature perception, respectively”

✓ located verbatim in the source · abstract, characters 423–572 · show in text ↑
t0

“sweet cherry”

✓ located verbatim in the source · title, characters 24–36 · show in text ↑

cold · molecular: expression · Prunus avium (sweet cherry)Results agree

3 papers: 3 positive, 0 negative or no effect

2 papers in the same direction as this paper

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  2. positive
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.