Abstract
Nitric oxide (NO) is a unique reactive nitrogen molecule with an array of signaling functions that modulates plant developmental processes and stress responses. To explore the mechanisms by which NO modulates root development, we used a pharmacological approach and NO-deficient mutants to unravel the role of NO in establishing auxin distribution patterns necessary for stem cell niche homeostasis. Using the NO synthase inhibitor and Arabidopsis (Arabidopsis thaliana) NO biosynthesis mutants (nitric oxide-associated1 [noa1], nitrate reductase1 [nia1] and nia2, and nia1 nia2 noa1), we show that depletion of NO in noa1 reduces primary root elongation and increases flavonol accumulation consistent with elevated reactive oxygen species levels. The elevated flavonols are required for the growth effect, because the transparent testa4 mutation reverses the noa1 mutant root elongation phenotype. In addition, noa1 and nia1 nia2 noa1 NO-deficient mutant roots display small root meristems with abnormal divisions. Concomitantly, auxin biosynthesis, transport, and signaling are perturbed. We further show that NO accumulates in cortex/endodermis stem cells and their precursor cells. In endodermal and cortical cells, the noa1 mutant acts synergistically to the effect of the wuschel-related homeobox5 mutation on the proximal meristem, suggesting that NO could play an important role in regulating stem cell decisions, which has been reported in animals.
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Claims 11
Claim · model-written summary
NO depletion in noa1 reduced primary root elongation.
- Taxon
- Arabidopsis
Arabidopsist0 - Material
- noa1e0
- Treatment
- depletion of NOe0
- Outcome
- primary root elongatione0
- Direction
- reducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“depletion of NO in noa1 reduces primary root elongation and increases flavonol accumulation”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NO depletion increased flavonol accumulation in noa1.
- Taxon
- ArabidopsisArabidopsist0
- Material
- noa1e0
- Treatment
- depletion of NOe0
- Outcome
- flavonol accumulatione0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“depletion of NO in noa1 reduces primary root elongation and increases flavonol accumulation”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Transparent testa4 mutation reversed the noa1 root elongation phenotype.
- Taxon
- ArabidopsisArabidopsist0
- Material
- noa1e0
- Treatment
- transparent testa4 mutatione0
- Outcome
- noa1 mutant root elongation phenotypee0
- Direction
- reversese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the transparent testa4 mutation reverses the noa1 mutant root elongation phenotype.”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Elevated flavonols are required for the noa1 growth effect.
- Taxon
- ArabidopsisArabidopsist0
- Compound
- elevated flavonolse0
- Outcome
- growth effecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The elevated flavonols are required for the growth effect”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NO-deficient noa1 and triple-mutant roots had small meristems.
- Taxon
- ArabidopsisArabidopsist0
- Material
- noa1 and nia1 nia2 noa1e0
- Organ / stage
- rootse0
- Outcome
- small root meristemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“noa1 and nia1 nia2 noa1 NO-deficient mutant roots display small root meristems with abnormal divisions.”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NO-deficient mutant roots had abnormal divisions.
- Taxon
- ArabidopsisArabidopsist0
- Material
- noa1 and nia1 nia2 noa1e0
- Organ / stage
- rootse0
- Outcome
- abnormal divisionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“noa1 and nia1 nia2 noa1 NO-deficient mutant roots display small root meristems with abnormal divisions.”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Auxin biosynthesis was perturbed.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- auxin biosynthesise0
- Direction
- perturbede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Concomitantly, auxin biosynthesis, transport, and signaling are perturbed.”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Auxin transport was perturbed.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- transporte0
- Direction
- perturbede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Concomitantly, auxin biosynthesis, transport, and signaling are perturbed.”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Auxin signaling was perturbed.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- signalinge0
- Direction
- perturbede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Concomitantly, auxin biosynthesis, transport, and signaling are perturbed.”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
NO accumulated in cortex/endodermis stem cells and precursors.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- cortex/endodermis stem cells and their precursor cellse0
- Outcome
- NO accumulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO accumulates in cortex/endodermis stem cells and their precursor cells.”
t0“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
The noa1 mutant acted synergistically with wuschel-related homeobox5 mutation on the proximal meristem.
- Taxon
- ArabidopsisArabidopsist0
- Material
- noa1 mutante0
- Gene
- wuschel-related homeobox5e0
- Outcome
- proximal meristeme0
- Direction
- synergisticallye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the noa1 mutant acts synergistically to the effect of the wuschel-related homeobox5 mutation on the proximal meristem”
t0“Arabidopsis (Arabidopsis thaliana)”
The same questions in other papers
nitric oxide · growth · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 3 in the opposite direction
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- negative
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