Abstract
Methyl 3-(4-hydroxyphenyl)propionate (MHPP) is a root exudate that functions as a nitrification inhibitor and as a modulator of the root system architecture (RSA) by inhibiting primary root (PR) elongation and promoting lateral root formation. However, the mechanism underlying MHPP-mediated modulation of the RSA remains unclear. Here, we report that MHPP inhibits PR elongation in Arabidopsis (Arabidopsis thaliana) by elevating the levels of auxin expression and signaling. MHPP induces an increase in auxin levels by up-regulating auxin biosynthesis, altering the expression of auxin carriers, and promoting the degradation of the auxin/indole-3-acetic acid family of transcriptional repressors. We found that MHPP-induced nitric oxide (NO) production promoted reactive oxygen species (ROS) accumulation in root tips. Suppressing the accumulation of NO or ROS alleviated the inhibitory effect of MHPP on PR elongation by weakening auxin responses and perception and by affecting meristematic cell division potential. Genetic analysis supported the phenotype described above. Taken together, our results indicate that MHPP modulates RSA remodeling via the NO/ROS-mediated auxin response pathway in Arabidopsis. Our study also revealed that MHPP significantly induced the accumulation of glucosinolates in roots, suggesting the diverse functions of MHPP in modulating plant growth, development, and stress tolerance in plants.
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Claims 10
Claim · model-written summary
MHPP inhibited primary root elongation through elevated auxin signaling.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- MHPPe0
- Compound
- auxine0
- Outcome
- PR elongatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MHPP inhibits PR elongation in Arabidopsis (Arabidopsis thaliana) by elevating the levels of auxin expression and signaling”
Claim · model-written summary
MHPP increased auxin levels by upregulating biosynthesis.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- MHPPe0
- Outcome
- auxin levelse0
- Qualifier
- up-regulating auxin biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MHPP induces an increase in auxin levels by up-regulating auxin biosynthesis”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MHPP altered expression of auxin carriers.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- MHPPe1
- Gene
- auxin carrierse0
- Outcome
- expression of auxin carrierse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“altering the expression of auxin carriers”
e1“MHPP induces an increase in auxin levels”
e2“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MHPP promoted degradation of auxin/indole-3-acetic acid transcriptional repressors.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- MHPPe1
- Gene
- auxin/indole-3-acetic acid family of transcriptional repressorse0
- Outcome
- degradation of the auxin/indole-3-acetic acid family of transcriptional repressorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“promoting the degradation of the auxin/indole-3-acetic acid family of transcriptional repressors”
e1“MHPP induces an increase in auxin levels”
e2“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MHPP induced NO production that promoted ROS accumulation in root tips.
- Taxon
- ArabidopsisArabidopsise1
- Organ / stage
- root tipse0
- Treatment
- MHPP-induced nitric oxide (NO) productione0
- Compound
- nitric oxide (NO)e0
- Outcome
- reactive oxygen species (ROS) accumulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MHPP-induced nitric oxide (NO) production promoted reactive oxygen species (ROS) accumulation in root tips”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Suppressing NO accumulation alleviated MHPP inhibition of primary root elongation.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Suppressing the accumulation of NOe0
- Outcome
- inhibitory effect of MHPP on PR elongatione0
- Direction
- alleviatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppressing the accumulation of NO or ROS alleviated the inhibitory effect of MHPP on PR elongation”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Suppressing ROS accumulation alleviated MHPP inhibition of primary root elongation.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Suppressing the accumulation of NO or ROSe0
- Outcome
- inhibitory effect of MHPP on PR elongatione0
- Direction
- alleviatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppressing the accumulation of NO or ROS alleviated the inhibitory effect of MHPP on PR elongation”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Suppression of NO or ROS weakened auxin responses and perception.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Suppressing the accumulation of NO or ROSe0
- Compound
- auxine0
- Outcome
- auxin responses and perceptione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppressing the accumulation of NO or ROS alleviated the inhibitory effect of MHPP on PR elongation by weakening auxin responses and perception and by affecting meristematic cell division potential”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
Suppression of NO or ROS affected meristematic cell division potential.
- Taxon
- ArabidopsisArabidopsise1
- Treatment
- Suppressing the accumulation of NO or ROSe0
- Outcome
- meristematic cell division potentiale0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suppressing the accumulation of NO or ROS alleviated the inhibitory effect of MHPP on PR elongation by weakening auxin responses and perception and by affecting meristematic cell division potential”
e1“Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MHPP significantly increased glucosinolate accumulation in roots.
- Taxon
- ArabidopsisArabidopsise1
- Organ / stage
- rootse0
- Treatment
- MHPPe0
- Outcome
- accumulation of glucosinolatese0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MHPP significantly induced the accumulation of glucosinolates in roots”
e1“Arabidopsis (Arabidopsis thaliana)”
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6 papers in the same direction as this paper · 2 in the opposite direction
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See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.