Abstract
Abstract Background Reactive oxygen species (ROS) and calcium ions (Ca 2+ ) are representative signals of plant wound responses. Wounding triggers cell fate transition in detached plant tissues and induces de novo root organogenesis. While the hormonal regulation of root organogenesis has been widely studied, the role of early wound signals including ROS and Ca 2+ remains largely unknown. Results We identified that ROS and Ca 2+ are required for de novo root organogenesis, but have different functions in Arabidopsis explants. The inhibition of the ROS and Ca 2+ signals delayed root development in detached leaves. Examination of the auxin signaling pathways indicated that ROS and Ca 2+ did not affect auxin biosynthesis and transport in explants. Additionally, the expression of key genes related to auxin signals during root organogenesis was not significantly affected by the inhibition of ROS and Ca 2+ signals. The addition of auxin partially restored the suppression of root development by the ROS inhibitor; however, auxin supplementation did not affect root organogenesis in Ca 2+ -depleted explants. Conclusions Our results indicate that, while both ROS and Ca 2+ are key molecules, at least in part of the auxin signals acts downstream of ROS signaling, and Ca 2+ acts downstream of auxin during de novo root organogenesis in leaf explants.
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Claims 8
Claim · model-written summary
ROS was required for de novo root organogenesis.
- Taxon
- Arabidopsis
Arabidopsist0 - Compound
- ROSe0
- Outcome
- de novo root organogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS and Ca 2+ are required for de novo root organogenesis”
t0“Arabidopsis explants”
Claim · model-written summary
Calcium was required for de novo root organogenesis.
- Taxon
- ArabidopsisArabidopsist0
- Compound
- Ca 2+e0
- Outcome
- de novo root organogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS and Ca 2+ are required for de novo root organogenesis”
t0“Arabidopsis explants”
Claim · model-written summary
ROS inhibition delayed root development in detached leaves.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- detached leavese0
- Treatment
- inhibition of the ROSe0
- Outcome
- root developmente0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inhibition of the ROS and Ca 2+ signals delayed root development in detached leaves.”
t0“Arabidopsis explants”
Claim · model-written summary
Calcium signal inhibition delayed root development in detached leaves.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- detached leavese0
- Treatment
- Ca 2+ signalse0
- Outcome
- root developmente0
- Direction
- delayede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The inhibition of the ROS and Ca 2+ signals delayed root development in detached leaves.”
t0“Arabidopsis explants”
Claim · model-written summary
ROS and calcium did not affect auxin biosynthesis and transport.
- Taxon
- ArabidopsisArabidopsist0
- Outcome
- auxin biosynthesis and transporte0
- Direction
- did not affecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS and Ca 2+ did not affect auxin biosynthesis and transport in explants”
t0“Arabidopsis explants”
Claim · model-written summary
Inhibiting ROS and calcium did not significantly affect expression of auxin signal genes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- inhibition of ROS and Ca 2+ signalse0
- Outcome
- expression of key genes related to auxin signalse0
- Direction
- not significantly affectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of key genes related to auxin signals during root organogenesis was not significantly affected by the inhibition of ROS and Ca 2+ signals”
t0“Arabidopsis explants”
Claim · model-written summary
Auxin partly restored root development under ROS inhibition.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- addition of auxine0
- Outcome
- root developmente0
- Direction
- partially restorede0
- Comparator
- ROS inhibitore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The addition of auxin partially restored the suppression of root development by the ROS inhibitor”
t0“Arabidopsis explants”
Claim · model-written summary
Auxin supplementation did not affect roots in calcium-depleted explants.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- auxin supplementatione0
- Outcome
- root organogenesise0
- Direction
- did not affecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“auxin supplementation did not affect root organogenesis in Ca 2+ -depleted explants”
t0“Arabidopsis explants”
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