Abstract
Lateral root (LR) emergence represents a highly coordinated process in which the plant hormone auxin plays a central role. Reactive oxygen species (ROS) have been proposed to function as important signals during auxin-regulated LR formation; however, their mode of action is poorly understood. Here, we report that Arabidopsis roots exposed to ROS show increased LR numbers due to the activation of LR pre-branch sites and LR primordia (LRP). Strikingly, ROS treatment can also restore LR formation in pCASP1:shy2-2 and aux1 lax3 mutant lines in which auxin-mediated cell wall accommodation and remodeling in cells overlying the sites of LR formation is disrupted. Specifically, ROS are deposited in the apoplast of these cells during LR emergence, following a spatiotemporal pattern that overlaps the combined expression domains of extracellular ROS donors of the RESPIRATORY BURST OXIDASE HOMOLOGS (RBOH). We also show that disrupting (or enhancing) expression of RBOH in LRP and/or overlying root tissues decelerates (or accelerates) the development and emergence of LRs. We conclude that RBOH-mediated ROS production facilitates LR outgrowth by promoting cell wall remodeling of overlying parental tissues.
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Claims 9
Claim · model-written summary
ROS exposure increased lateral root numbers in Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsise0 - Organ / stage
- rootse0
- Treatment
- ROSe0
- Outcome
- LR numberse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis roots exposed to ROS show increased LR numbers due to the activation of LR pre-branch sites and LR primordia (LRP).”
Claim · model-written summary
ROS exposure activated lateral root pre-branch sites and primordia.
- Taxon
- ArabidopsisArabidopsise0
- Organ / stage
- rootse0
- Treatment
- ROSe0
- Outcome
- activation of LR pre-branch sites and LR primordia (LRP)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Arabidopsis roots exposed to ROS show increased LR numbers due to the activation of LR pre-branch sites and LR primordia (LRP).”
Claim · model-written summary
ROS treatment restored lateral root formation in pCASP1:shy2-2.
- Taxon
- ArabidopsisArabidopsist0
- Material
- pCASP1:shy2-2e0
- Treatment
- ROS treatmente0
- Outcome
- LR formatione0
- Direction
- restoree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS treatment can also restore LR formation in pCASP1:shy2-2 and aux1 lax3 mutant lines”
t0“Arabidopsis”
Claim · model-written summary
ROS treatment restored lateral root formation in aux1 lax3 mutants.
- Taxon
- ArabidopsisArabidopsist0
- Material
- aux1 lax3e0
- Treatment
- ROS treatmente0
- Outcome
- LR formatione0
- Direction
- restoree0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS treatment can also restore LR formation in pCASP1:shy2-2 and aux1 lax3 mutant lines”
t0“Arabidopsis”
Claim · model-written summary
ROS was deposited in the apoplast of cells overlying lateral root sites during emergence.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- apoplaste0
- Outcome
- ROS are deposited in the apoplast of these cells during LR emergencee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ROS are deposited in the apoplast of these cells during LR emergence, following a spatiotemporal pattern that overlaps the combined expression domains of extracellular ROS donors of the RESPIRATORY BURST OXIDASE HOMOLOGS (RBOH).”
t0“Arabidopsis”
Claim · model-written summary
The ROS deposition pattern overlapped RBOH expression domains.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RESPIRATORY BURST OXIDASE HOMOLOGS (RBOH)e0
- Outcome
- spatiotemporal patterne0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a spatiotemporal pattern that overlaps the combined expression domains of extracellular ROS donors of the RESPIRATORY BURST OXIDASE HOMOLOGS (RBOH)”
t0“Arabidopsis”
Claim · model-written summary
Disrupting RBOH expression decelerated lateral root development and emergence.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- disruptinge0
- Gene
- RBOHe0
- Outcome
- development and emergence of LRse0
- Direction
- deceleratese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disrupting (or enhancing) expression of RBOH in LRP and/or overlying root tissues decelerates (or accelerates) the development and emergence of LRs”
t0“Arabidopsis”
Claim · model-written summary
Enhancing RBOH expression accelerated lateral root development and emergence.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- enhancinge0
- Gene
- RBOHe0
- Outcome
- development and emergence of LRse0
- Direction
- acceleratese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disrupting (or enhancing) expression of RBOH in LRP and/or overlying root tissues decelerates (or accelerates) the development and emergence of LRs”
t0“Arabidopsis”
Claim · model-written summary
The authors conclude that RBOH-mediated ROS production facilitates lateral root outgrowth by promoting cell-wall remodeling.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- RBOHe0
- Outcome
- LR outgrowthe0
- Qualifier
- promoting cell wall remodeling of overlying parental tissuese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RBOH-mediated ROS production facilitates LR outgrowth by promoting cell wall remodeling of overlying parental tissues.”
t0“Arabidopsis”
The same questions in other papers
reactive oxygen species · growth · ArabidopsisPositive and negative results
6 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
5 papers more in this group
See all 9 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.