Abstract
The earliest proteolytic event affecting most proteins is the excision of the initiating Met (NME). This is an essential and ubiquitous cotranslational process tightly regulated in all eukaryotes. Currently, the effects of NME on unknown complex cellular networks and the ways in which its inhibition leads to developmental defects and cell growth arrest remain poorly understood. Here, we provide insight into the earliest molecular mechanisms associated with the inhibition of the NME process in Arabidopsis thaliana. We demonstrate that the developmental defects induced by NME inhibition are caused by an increase in cellular proteolytic activity, primarily induced by an increase in the number of proteins targeted for rapid degradation. This deregulation drives, through the increase of the free amino acids pool, a perturbation of the glutathione homeostasis, which corresponds to the earliest limiting, reversible step promoting the phenotype. We demonstrate that these effects are universally conserved and that the reestablishment of the appropriate glutathione status restores growth and proper development in various organisms. Finally, we describe a novel integrated model in which NME, protein N-alpha-acylation, proteolysis, and glutathione homeostasis operate in a sequentially regulated mechanism that directs both growth and development.
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Claims 7
Claim · model-written summary
NME inhibition caused developmental defects through increased cellular proteolytic activity.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianat0 - Treatment
- NME inhibitione0
- Outcome
- developmental defectse0
- Qualifier
- increase in cellular proteolytic activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the developmental defects induced by NME inhibition are caused by an increase in cellular proteolytic activity”
t0“NME process in Arabidopsis thaliana”
Claim · model-written summary
An increased number of proteins targeted for rapid degradation primarily drove proteolytic activity.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- increase in cellular proteolytic activitye0
- Qualifier
- increase in the number of proteins targeted for rapid degradatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an increase in cellular proteolytic activity, primarily induced by an increase in the number of proteins targeted for rapid degradation.”
t0“NME process in Arabidopsis thaliana”
Claim · model-written summary
Deregulated proteolysis increased the free amino acid pool and perturbed glutathione homeostasis.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- perturbation of the glutathione homeostasise0
- Qualifier
- increase of the free amino acids poole0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This deregulation drives, through the increase of the free amino acids pool, a perturbation of the glutathione homeostasis”
t0“NME process in Arabidopsis thaliana”
Claim · model-written summary
The glutathione perturbation was the earliest limiting and reversible step promoting the phenotype.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- phenotypee0
- Qualifier
- earliest limiting, reversible stepe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“which corresponds to the earliest limiting, reversible step promoting the phenotype.”
t0“NME process in Arabidopsis thaliana”
Claim · model-written summary
These effects were conserved across organisms.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- these effects are universally conservede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“these effects are universally conserved”
t0“NME process in Arabidopsis thaliana”
Claim · model-written summary
Restoring glutathione status restored growth and proper development across organisms.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Treatment
- reestablishment of the appropriate glutathione statuse0
- Outcome
- growth and proper developmente0
- Direction
- restorese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the reestablishment of the appropriate glutathione status restores growth and proper development in various organisms.”
t0“NME process in Arabidopsis thaliana”
Claim · model-written summary
The authors propose a sequential model linking NME, protein N-alpha-acylation, proteolysis and glutathione homeostasis to growth and development.
- Taxon
- Arabidopsis thalianaArabidopsis thalianat0
- Outcome
- novel integrated modele0
Not reportedComparatorValue
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a novel integrated model in which NME, protein N-alpha-acylation, proteolysis, and glutathione homeostasis operate in a sequentially regulated mechanism that directs both growth and development.”
t0“NME process in Arabidopsis thaliana”
The same questions in other papers
glutathione · growth · Arabidopsis thaliana (thale cress)Positive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.