Abstract
Using NO specific probe (MNIP-Cu), rapid nitric oxide (NO) accumulation as a response to auxin (IAA) treatment has been observed in the protoplasts from the hypocotyls of sunflower seedlings (Helianthus annuus L.). Incubation of protoplasts in presence of NPA (auxin efflux blocker) and PTIO (NO scavenger) leads to significant reduction in NO accumulation, indicating that NO signals represent an early signaling event during auxin-induced response. A surge in NO production has also been demonstrated in whole hypocotyl explants showing adventitious root (AR) development. Evidence of tyrosine nitration of cytosolic proteins as a consequence of NO accumulation has been provided by western blot analysis and immunolocalization in the sections of AR producing hypocotyl segments. Most abundant anti-nitrotyrosine labeling is evident in proteins ranging from 25-80 kDa. Tyrosine nitration of a particular protein (25 kDa) is completely absent in presence of NPA (which suppresses AR formation). Similar lack of tyrosine nitration of this protein is also evident in other conditions which do not allow AR differentiation. Immunofluorescent localization experiments have revealed that non-inductive treatments (such as PTIO) for AR develpoment from hypocotyl segments coincide with symplastic and apoplastic localization of tyrosine nitrated proteins in the xylem elements, in contrast with negligible (and mainly apoplastic) nitration of proteins in the interfascicular cells and phloem elements. Application of NPA does not affect tyrosine nitration of proteins even in the presence of an external source of NO (SNP). Tyrosine nitrated proteins are abundant around the nuclei in the actively dividing cells of the root primordium. Thus, NO-modulated rapid response to IAA treatment through differential distribution of tyrosine nitrated proteins is evident as an inherent aspect of the AR development.
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Claims 10
Claim · model-written summary
IAA rapidly increased nitric oxide in sunflower hypocotyl protoplasts.
- Taxon
- sunflower seedlings (Helianthus annuus L.)
Helianthus annuuse0 - Organ / stage
- hypocotylse0
- Treatment
- auxin (IAA) treatmente0
- Outcome
- nitric oxide (NO) accumulatione0
- Direction
- accumulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rapid nitric oxide (NO) accumulation as a response to auxin (IAA) treatment has been observed in the protoplasts from the hypocotyls of sunflower seedlings (Helianthus annuus L.)”
Claim · model-written summary
NPA and PTIO reduced nitric oxide accumulation in sunflower protoplasts.
- Taxon
- sunflowerHelianthus annuust0
- Treatment
- NPA (auxin efflux blocker) and PTIO (NO scavenger)e0
- Outcome
- NO accumulatione0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Incubation of protoplasts in presence of NPA (auxin efflux blocker) and PTIO (NO scavenger) leads to significant reduction in NO accumulation”
t0“sunflower hypocotyls”
Claim · model-written summary
Nitric oxide production surged in sunflower hypocotyl explants developing adventitious roots.
- Taxon
- sunflowerHelianthus annuust0
- Organ / stage
- whole hypocotyl explantse0
- Outcome
- NO productione0
- Direction
- surgee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A surge in NO production has also been demonstrated in whole hypocotyl explants showing adventitious root (AR) development”
t0“sunflower hypocotyls”
Claim · model-written summary
Cytosolic proteins in rooting sunflower hypocotyl segments showed tyrosine nitration.
- Taxon
- sunflowerHelianthus annuust0
- Organ / stage
- AR producing hypocotyl segmentse0
- Outcome
- tyrosine nitration of cytosolic proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Evidence of tyrosine nitration of cytosolic proteins as a consequence of NO accumulation has been provided by western blot analysis and immunolocalization in the sections of AR producing hypocotyl segments”
t0“sunflower hypocotyls”
Claim · model-written summary
The most abundant nitrotyrosine labeling was on 25-80 kDa proteins.
- Taxon
- sunflowerHelianthus annuust0
- Outcome
- anti-nitrotyrosine labelinge0
- Value
- 25-80 kDae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most abundant anti-nitrotyrosine labeling is evident in proteins ranging from 25-80 kDa”
t0“sunflower hypocotyls”
Claim · model-written summary
NPA eliminated tyrosine nitration of a 25 kDa protein during sunflower adventitious root formation.
- Taxon
- sunflowerHelianthus annuust0
- Treatment
- NPAe0
- Outcome
- Tyrosine nitration of a particular proteine0
- Direction
- completely absente0
- Value
- 25 kDae0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tyrosine nitration of a particular protein (25 kDa) is completely absent in presence of NPA (which suppresses AR formation)”
t0“sunflower hypocotyls”
Claim · model-written summary
NPA suppressed adventitious root formation in sunflower hypocotyls.
- Taxon
- sunflowerHelianthus annuust0
- Treatment
- NPAe0
- Outcome
- AR formatione0
- Direction
- suppressese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NPA (which suppresses AR formation)”
t0“sunflower hypocotyls”
Claim · model-written summary
Noninductive PTIO treatment coincided with tyrosine nitrated proteins in sunflower xylem elements and negligible nitration in interfascicular and phloem cells.
- Taxon
- sunflowerHelianthus annuust0
- Organ / stage
- xylem elementse0
- Treatment
- PTIOe0
- Outcome
- localization of tyrosine nitrated proteinse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“non-inductive treatments (such as PTIO) for AR develpoment from hypocotyl segments coincide with symplastic and apoplastic localization of tyrosine nitrated proteins in the xylem elements, in contrast with negligible (and mainly apoplastic) nitration of proteins in the interfascicular cells and phloem elements”
t0“sunflower hypocotyls”
Claim · model-written summary
NPA did not affect protein tyrosine nitration despite an external nitric oxide source.
- Taxon
- sunflowerHelianthus annuust0
- Treatment
- NPAe0
- Outcome
- tyrosine nitration of proteinse0
- Direction
- does not affecte0
- Qualifier
- external source of NO (SNP)e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Application of NPA does not affect tyrosine nitration of proteins even in the presence of an external source of NO (SNP)”
t0“sunflower hypocotyls”
Claim · model-written summary
Tyrosine nitrated proteins accumulated around nuclei in dividing root primordium cells.
- Taxon
- sunflowerHelianthus annuust0
- Organ / stage
- root primordiume0
- Outcome
- Tyrosine nitrated proteinse0
- Direction
- abundante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Tyrosine nitrated proteins are abundant around the nuclei in the actively dividing cells of the root primordium”
t0“sunflower hypocotyls”
The same questions in other papers
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- no effect
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1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.