Abstract
Overexpression of a tobacco glutathione S-transferase with glutathione peroxidase activity (GST/GPX) in transgenic tobacco (Nicotiana tabacum L.) enhanced seedling growth under a variety of stressful conditions. In addition to increased GST and GPX activity, transgenic GST/GPX-expressing (GST+) seedlings had elevated levels of monodehydroascorbate reductase activity. GST+ seedlings also contained higher levels of glutathione and ascorbate than wild-type seedlings and the glutathione pools were more oxidized. Thermal or salt-stress treatments that inhibited the growth of wild-type seedlings also caused increased levels of lipid peroxidation. These treatments had less effect on the growth of GST+ seedling growth and did not lead to increased lipid peroxidation. Stress-induced damage resulted in reduced metabolic activity in wild-type seedlings while GST+ seedlings maintained metabolic activity levels comparable to seedlings grown under control conditions. These results indicate that overexpression of GST/GPX in transgenic tobacco seedlings provides increased glutathione-dependent peroxide scavenging and alterations in glutathione and ascorbate metabolism that lead to reduced oxidative damage. We conclude that this protective effect is primarily responsible for the ability of GST+ seedlings to maintain growth under stressful conditions.
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Claims 13
Claim · model-written summary
GST/GPX overexpression enhanced tobacco seedling growth under stress.
- Taxon
- tobacco
Nicotiana tabacume0 - Treatment
- Overexpression of a tobacco glutathione S-transferase with glutathione peroxidase activity (GST/GPX)e0
- Gene
- GST/GPXe0
- Outcome
- seedling growthe0
- Direction
- enhancede0
- Qualifier
- stressful conditionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of a tobacco glutathione S-transferase with glutathione peroxidase activity (GST/GPX) in transgenic tobacco (Nicotiana tabacum L.) enhanced seedling growth under a variety of stressful conditions”
Claim · model-written summary
GST/GPX-expressing seedlings had increased GST and GPX activity.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- GST+e0
- Gene
- GST and GPXe0
- Outcome
- GST and GPX activitye0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“increased GST and GPX activity, transgenic GST/GPX-expressing (GST+) seedlings”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
GST+ seedlings had elevated monodehydroascorbate reductase activity.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- GST+e0
- Gene
- monodehydroascorbate reductasee0
- Outcome
- monodehydroascorbate reductase activitye0
- Direction
- elevatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transgenic GST/GPX-expressing (GST+) seedlings had elevated levels of monodehydroascorbate reductase activity”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
GST+ seedlings had higher glutathione and ascorbate levels than wild type.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- GST+e0
- Outcome
- levels of glutathione and ascorbatee0
- Direction
- highere0
- Comparator
- wild-type seedlingse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GST+ seedlings also contained higher levels of glutathione and ascorbate than wild-type seedlings”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
GST+ seedlings had more oxidized glutathione pools.
- Taxon
- tobaccoNicotiana tabacume2
- Material
- GST+e1
- Outcome
- glutathione poolse0
- Direction
- more oxidizede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the glutathione pools were more oxidized”
e1“GST+ seedlings also contained higher levels of glutathione and ascorbate than wild-type seedlings”
e2“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Thermal or salt stress inhibited wild-type seedling growth.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- wild-type seedlingse0
- Treatment
- Thermal or salt-stress treatmentse0
- Outcome
- growth of wild-type seedlingse0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thermal or salt-stress treatments that inhibited the growth of wild-type seedlings”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Thermal or salt stress increased lipid peroxidation in wild-type seedlings.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- wild-type seedlingse0
- Treatment
- Thermal or salt-stress treatmentse0
- Outcome
- levels of lipid peroxidatione0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Thermal or salt-stress treatments that inhibited the growth of wild-type seedlings also caused increased levels of lipid peroxidation”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Thermal or salt stress had less effect on GST+ seedling growth.
- Taxon
- tobaccoNicotiana tabacume2
- Material
- GST+e0
- Treatment
- Thermal or salt-stress treatmentse1
- Outcome
- growth of GST+ seedling growthe0
- Direction
- less effecte0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These treatments had less effect on the growth of GST+ seedling growth”
e1“Thermal or salt-stress treatments”
e2“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Thermal or salt stress did not increase lipid peroxidation in GST+ seedlings.
- Taxon
- tobaccoNicotiana tabacume2
- Material
- GST+e0
- Treatment
- Thermal or salt-stress treatmentse1
- Outcome
- lipid peroxidatione0
- Direction
- did not lead to increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“These treatments had less effect on the growth of GST+ seedling growth and did not lead to increased lipid peroxidation”
e1“Thermal or salt-stress treatments”
e2“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Stress damage reduced metabolic activity in wild-type seedlings.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- wild-type seedlingse0
- Treatment
- Stress-induced damagee0
- Outcome
- metabolic activitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Stress-induced damage resulted in reduced metabolic activity in wild-type seedlings”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
GST+ seedlings maintained metabolic activity comparable to control-grown seedlings under stress.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- GST+e0
- Outcome
- metabolic activity levelse0
- Direction
- maintainede0
- Comparator
- seedlings grown under control conditionse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GST+ seedlings maintained metabolic activity levels comparable to seedlings grown under control conditions”
e1“tobacco (Nicotiana tabacum L.)”
Claim · model-written summary
Authors attribute reduced oxidative damage to enhanced glutathione-dependent peroxide scavenging and altered antioxidant metabolism.
- Taxon
- tobaccoNicotiana tabacume0
- Gene
- GST/GPXe0
- Outcome
- oxidative damagee0
- Qualifier
- glutathione-dependent peroxide scavenging and alterations in glutathione and ascorbate metabolisme0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“overexpression of GST/GPX in transgenic tobacco seedlings provides increased glutathione-dependent peroxide scavenging and alterations in glutathione and ascorbate metabolism that lead to reduced oxidative damage”
Claim · model-written summary
Authors attribute maintained growth under stress primarily to the protective antioxidant effect.
- Taxon
- tobaccoNicotiana tabacume1
- Material
- GST+e0
- Outcome
- maintain growth under stressful conditionse0
- Qualifier
- this protective effecte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this protective effect is primarily responsible for the ability of GST+ seedlings to maintain growth under stressful conditions”
e1“tobacco (Nicotiana tabacum L.)”
The same questions in other papers
salinity · physiology: antioxidant enzymes · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
- positive
- negative
- no effect
salinity · physiology: oxidative · Nicotiana tabacum (tobacco)Positive and negative results
4 papers in the same direction as this paper · 3 in the opposite direction
- negative
- negative
- negative
4 papers more in this group
See all 8 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.