Abstract
Blue light (BL) rapidly and strongly inhibits hypocotyl elongation during the photomorphogenic response known as de-etiolation, the transformation of a dark-grown seedling into a pigmented, photoautotrophic organism. In Arabidopsis thaliana, high-resolution studies of hypocotyl growth accomplished by computer-assisted electronic image capture and analysis revealed that inhibition occurs in two genetically independent phases, the first beginning within 30 sec of illumination. The present work demonstrates that phototropin (nph1), the photoreceptor responsible for phototropism, is largely responsible for the initial, rapid inhibition. Signaling from phototropin during the curvature response is dependent upon interaction with NPH3, but the results presented here demonstrate that NPH3 is not necessary for phototropin-dependent growth inhibition. Activation of anion channels, which transiently depolarizes the plasma membrane within seconds of BL, is an early event in the cryptochrome signaling pathway leading to a phase of growth inhibition that replaces the transient phototropin-dependent phase after approximately 30 min of BL. Surprisingly, cry1 and cry2 were found to contribute equally and non-redundantly to anion-channel activation and to growth inhibition between 30 and 120 min of BL. Inspection of the inhibition kinetics displayed by nph1 and nph1cry1 mutants revealed that the cryptochrome phase of inhibition is delayed in seedlings lacking phototropin. This result indicates that BL-activation of phototropin influences cryptochrome signaling leading to growth inhibition. Mutations in the NPQ1 gene, which inhibit BL-induced stomatal opening, do not affect any aspect of the growth inhibition within the first 120 min examined here, and NPQ1 does not affect the activation of anion channels.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 9
Claim · model-written summary
Blue-light hypocotyl growth inhibition occurs in two genetically independent phases, the first beginning within 30 seconds of illumination.
- Taxon
- Arabidopsis thaliana
Arabidopsis thalianae0 - Outcome
- inhibitione1
- Qualifier
- occurs in two genetically independent phasese1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, high-resolution studies of hypocotyl growth”
e1“revealed that inhibition occurs in two genetically independent phases, the first beginning within 30 sec of illumination”
Claim · model-written summary
Phototropin (nph1) is largely responsible for the initial, rapid blue-light inhibition of hypocotyl growth.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- phototropin (nph1)e2
- Outcome
- initial, rapid inhibitione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, high-resolution studies of hypocotyl growth”
e2“The present work demonstrates that phototropin (nph1), the photoreceptor responsible for phototropism, is largely responsible for the initial, rapid inhibition”
Claim · model-written summary
NPH3 is not necessary for phototropin-dependent growth inhibition.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- NPH3e3
- Outcome
- phototropin-dependent growth inhibitione3
- Qualifier
- not necessarye3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, high-resolution studies of hypocotyl growth”
e3“the results presented here demonstrate that NPH3 is not necessary for phototropin-dependent growth inhibition”
Claim · model-written summary
Activation of anion channels is an early event in the cryptochrome signalling pathway leading to growth inhibition.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- cryptochromee4
- Outcome
- growth inhibitione4
- Qualifier
- Activation of anion channelse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, high-resolution studies of hypocotyl growth”
e4“Activation of anion channels, which transiently depolarizes the plasma membrane within seconds of BL, is an early event in the cryptochrome signaling pathway leading to a phase of growth inhibition”
Claim · model-written summary
Cryptochrome 1 and cryptochrome 2 contribute equally and non-redundantly to anion-channel activation and growth inhibition between 30 and 120 min of blue light.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- cry1 and cry2e5
- Outcome
- growth inhibitione5
- Qualifier
- contribute equally and non-redundantlye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, high-resolution studies of hypocotyl growth”
e5“cry1 and cry2 were found to contribute equally and non-redundantly to anion-channel activation and to growth inhibition between 30 and 120 min of BL”
Claim · model-written summary
The cryptochrome phase of growth inhibition is delayed in seedlings lacking phototropin.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- seedlings lacking phototropine6
- Outcome
- cryptochrome phase of inhibitione6
- Direction
- delayede6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, high-resolution studies of hypocotyl growth”
e6“Inspection of the inhibition kinetics displayed by nph1 and nph1cry1 mutants revealed that the cryptochrome phase of inhibition is delayed in seedlings lacking phototropin”
Claim · model-written summary
Blue-light activation of phototropin influences cryptochrome signalling that leads to growth inhibition.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- phototropine7
- Outcome
- growth inhibitione7
- Qualifier
- BL-activation of phototropin influences cryptochrome signalinge7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, high-resolution studies of hypocotyl growth”
e7“This result indicates that BL-activation of phototropin influences cryptochrome signaling leading to growth inhibition”
Claim · model-written summary
NPQ1 mutations do not affect any aspect of blue-light growth inhibition within the first 120 min.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Treatment
- Mutations in the NPQ1 genee1
- Gene
- NPQ1e1
- Outcome
- growth inhibition within the first 120 mine1
- Direction
- do not affecte1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, high-resolution studies of hypocotyl growth”
e1“Mutations in the NPQ1 gene, which inhibit BL-induced stomatal opening, do not affect any aspect of the growth inhibition within the first 120 min examined here”
Claim · model-written summary
NPQ1 does not affect the activation of anion channels in blue light.
- Taxon
- Arabidopsis thalianaArabidopsis thalianae0
- Gene
- NPQ1e1
- Outcome
- activation of anion channelse1
- Direction
- does not affecte1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In Arabidopsis thaliana, high-resolution studies of hypocotyl growth”
e1“NPQ1 does not affect the activation of anion channels”
The same questions in other papers
loss of function · disease · Arabidopsis thaliana (thale cress)Positive and negative results
50 papers in the same direction as this paper · 31 in the opposite direction
- negative
- positive
- positive
78 papers more in this group
See all 82 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.