Abstract
The auxin indole-3-acetic acid (IAA) has been shown to promote the biosynthesis of the active gibberellin (GA(1)) in shoots of pea (Pisum sativum). We used northern analysis to investigate the timing of IAA-induced changes in transcript levels of PsGA3ox1 (Mendel's LE), PsGA2ox1, PsGA2ox2, and PsGA20ox1, key genes for the later stages of GA(1) biosynthesis and metabolism in pea. Rapid (2-4 h) changes occurred in the transcript levels of PsGA3ox1, PsGA2ox1, and PsGA2ox2 after treatment with IAA. [(14)C]GA(1) metabolism studies in decapitated shoots indicated that IAA inhibits GA(1) deactivation, consistent with the suppression of PsGA2ox1 (SLN) transcript levels by IAA. Studies with the sln mutant also indicated that PsGA2ox1 activity is involved in GA(1) deactivation in decapitated shoots. Culture of excised internode tissue in the presence of auxin clearly demonstrated that internode tissue is a site of GA(1) biosynthesis per se. Excised internode tissue cultured in the presence/absence of cycloheximide showed that de novo protein synthesis is required for IAA-induced increases in PsGA3ox1 transcript levels. Auxin dose response studies indicated that IAA concentration is a critical determinant of GA(1) biosynthesis over 1 to 2 orders of magnitude, and a range of auxins was shown to affect the GA pathway.
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Claims 9
Claim · model-written summary
IAA promoted GA(1) biosynthesis in pea shoots.
- Taxon
- pea (Pisum sativum)
Lathyrus oleraceuse0 - Organ / stage
- shootse0
- Treatment
- indole-3-acetic acid (IAA)e0
- Outcome
- biosynthesis of the active gibberellin (GA(1))e0
- Direction
- promotee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The auxin indole-3-acetic acid (IAA) has been shown to promote the biosynthesis of the active gibberellin (GA(1)) in shoots of pea (Pisum sativum)”
Claim · model-written summary
IAA rapidly changed PsGA3ox1, PsGA2ox1 and PsGA2ox2 transcript levels within 2-4 h.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- IAAe0
- Outcome
- transcript levels of PsGA3ox1, PsGA2ox1, and PsGA2ox2e0
- Value
- 2-4 he0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Rapid (2-4 h) changes occurred in the transcript levels of PsGA3ox1, PsGA2ox1, and PsGA2ox2 after treatment with IAA”
t0“Pea”
Claim · model-written summary
IAA inhibited GA(1) deactivation in decapitated pea shoots.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- IAAe0
- Outcome
- GA(1) deactivatione0
- Direction
- inhibitse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA inhibits GA(1) deactivation”
t0“Pea”
Claim · model-written summary
IAA suppressed PsGA2ox1 transcript levels in pea.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- IAAe0
- Outcome
- PsGA2ox1 (SLN) transcript levelse0
- Direction
- suppressione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppression of PsGA2ox1 (SLN) transcript levels by IAA”
t0“Pea”
Claim · model-written summary
PsGA2ox1 activity was implicated in GA(1) deactivation in decapitated pea shoots.
- Taxon
- PeaLathyrus oleraceust0
- Organ / stage
- decapitated shootse0
- Gene
- PsGA2ox1e0
- Outcome
- GA(1) deactivatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“PsGA2ox1 activity is involved in GA(1) deactivation in decapitated shoots”
t0“Pea”
Claim · model-written summary
Excised pea internodes were a site of GA(1) biosynthesis.
- Taxon
- PeaLathyrus oleraceust0
- Organ / stage
- internode tissuee0
- Outcome
- GA(1) biosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“internode tissue is a site of GA(1) biosynthesis per se”
t0“Pea”
Claim · model-written summary
De novo protein synthesis was required for IAA-induced increases in PsGA3ox1 transcripts in pea internodes.
- Taxon
- PeaLathyrus oleraceust0
- Gene
- PsGA3ox1e0
- Outcome
- PsGA3ox1 transcript levelse0
- Qualifier
- de novo protein synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“de novo protein synthesis is required for IAA-induced increases in PsGA3ox1 transcript levels”
t0“Pea”
Claim · model-written summary
IAA concentration determined GA(1) biosynthesis over 1 to 2 orders of magnitude in pea.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- IAA concentratione0
- Outcome
- GA(1) biosynthesise0
- Value
- 1 to 2 orders of magnitudee0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA concentration is a critical determinant of GA(1) biosynthesis over 1 to 2 orders of magnitude”
t0“Pea”
Claim · model-written summary
A range of auxins affected the GA pathway in pea.
- Taxon
- PeaLathyrus oleraceust0
- Treatment
- a range of auxinse0
- Outcome
- GA pathwaye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a range of auxins was shown to affect the GA pathway”
t0“Pea”
The same questions in other papers
indole-3-acetic acid · molecular: metabolites · Lathyrus oleraceus (pea)Positive and negative results
11 papers in the same direction as this paper · 6 in the opposite direction · 1 reporting no difference from an alternative
- negative
- negative
- negative
15 papers more in this group
See all 19 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.