Abstract
Development of pathogen-resistant crops, such as fungus-resistant cotton, has significantly reduced chemical application and improved crop yield and quality. However, the mechanism of resistance to cotton pathogens such as Verticillium dahliae is still poorly understood. In this study, we characterized a cotton gene (HDTF1) that was isolated following transcriptome profiling during the resistance response of cotton to V. dahliae. HDTF1 putatively encodes a homeodomain transcription factor, and its expression was found to be down-regulated in cotton upon inoculation with V. dahliae and Botrytis cinerea. To characterise the involvement of HDTF1 in the response to these pathogens, we used virus-induced gene silencing (VIGS) to generate HDTF1-silenced cotton. VIGS reduction in HDTF1 expression significantly enhanced cotton plant resistance to both pathogens. HDTF1 silencing resulted in activation of jasmonic acid (JA)-mediated signaling and JA accumulation. However, the silenced plants were not altered in the accumulation of salicylic acid (SA) or the expression of marker genes associated with SA signaling. These results suggest that HDTF1 is a negative regulator of the JA pathway, and resistance to V. dahliae and B. cinerea can be engineered by activation of JA signaling.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
HDTF1 putatively encodes a homeodomain transcription factor.
- Taxon
- cotton
Gossypium hirsutume1 - Gene
- HDTF1e0
- Outcome
- homeodomain transcription factore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HDTF1 putatively encodes a homeodomain transcription factor”
e1“cotton gene (HDTF1)”
Claim · model-written summary
Cotton HDTF1 expression decreased after inoculation with V. dahliae.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- inoculation with V. dahliaee0
- Interactor
- V. dahliae
Verticillium dahliaee0pathogen - Gene
- HDTF1e1
- Outcome
- expressione0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression was found to be down-regulated in cotton upon inoculation with V. dahliae and Botrytis cinerea”
e1“cotton gene (HDTF1)”
Claim · model-written summary
Cotton HDTF1 expression decreased after inoculation with Botrytis cinerea.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- inoculation with V. dahliae and Botrytis cinereae0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Gene
- HDTF1e1
- Outcome
- expressione0
- Direction
- down-regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“its expression was found to be down-regulated in cotton upon inoculation with V. dahliae and Botrytis cinerea”
e1“cotton gene (HDTF1)”
Claim · model-written summary
HDTF1 silencing enhanced cotton resistance to V. dahliae.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- VIGS reduction in HDTF1 expressione0
- Interactor
- Verticillium dahliaeVerticillium dahliaee1pathogen
- Gene
- HDTF1e0
- Outcome
- cotton plant resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VIGS reduction in HDTF1 expression significantly enhanced cotton plant resistance to both pathogens”
e1“Verticillium dahliae and Botrytis cinerea in cotton”
Claim · model-written summary
HDTF1 silencing enhanced cotton resistance to Botrytis cinerea.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- VIGS reduction in HDTF1 expressione0
- Interactor
- Botrytis cinereaBotrytis cinereae1pathogen
- Gene
- HDTF1e0
- Outcome
- cotton plant resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“VIGS reduction in HDTF1 expression significantly enhanced cotton plant resistance to both pathogens”
e1“Verticillium dahliae and Botrytis cinerea in cotton”
Claim · model-written summary
HDTF1 silencing activated JA-mediated signaling.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- HDTF1 silencinge0
- Gene
- HDTF1e0
- Outcome
- jasmonic acid (JA)-mediated signalinge0
- Direction
- activatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HDTF1 silencing resulted in activation of jasmonic acid (JA)-mediated signaling”
e1“cotton gene (HDTF1)”
Claim · model-written summary
HDTF1 silencing increased JA accumulation.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- HDTF1 silencinge0
- Outcome
- JA accumulatione0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HDTF1 silencing resulted in activation of jasmonic acid (JA)-mediated signaling and JA accumulation”
e1“cotton gene (HDTF1)”
Claim · model-written summary
HDTF1-silenced cotton did not show altered SA accumulation or SA-associated marker gene expression.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- silenced plantse0
- Outcome
- accumulation of salicylic acid (SA) or the expression of marker genes associated with SA signalinge0
- Direction
- not alterede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the silenced plants were not altered in the accumulation of salicylic acid (SA) or the expression of marker genes associated with SA signaling”
e1“cotton gene (HDTF1)”
Claim · model-written summary
The authors infer that HDTF1 negatively regulates the JA pathway.
- Taxon
- cottonGossypium hirsutume1
- Gene
- HDTF1e0
- Outcome
- JA pathwaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HDTF1 is a negative regulator of the JA pathway”
e1“cotton gene (HDTF1)”
Claim · model-written summary
The authors propose engineering cotton resistance by activating JA signaling.
- Taxon
- cottonGossypium hirsutume1
- Outcome
- resistance to V. dahliae and B. cinerea can be engineered by activation of JA signalinge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“resistance to V. dahliae and B. cinerea can be engineered by activation of JA signaling”
e1“cotton gene (HDTF1)”
The same questions in other papers
loss of function · physiology: hormones · Gossypium hirsutum (cotton)Positive and negative results
4 papers in the same direction as this paper · 9 in the opposite direction
- negative
- negative
- negative
10 papers more in this group
See all 14 papers →loss of function · Verticillium dahliae · Gossypium hirsutum (cotton)Positive and negative results
33 papers in the same direction as this paper · 59 in the opposite direction
- negative
- negative
- no effect
89 papers more in this group
See all 93 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.