Abstract
Verticillium wilt causes massive annual losses of cotton yield, but the mechanism of cotton resistance to Verticillium dahliae is complex and poorly understood. In this study, a comparative proteomic analysis was performed in resistant cotton (Gossypium barbadense cv7124) on infection with V. dahliae. A total of 188 differentially expressed proteins were identified by mass spectrometry (MALDI-TOF/TOF) analysis and could be classified into 17 biological processes based on Gene Ontology annotation. Most of these proteins were implicated in stimulus response, cellular processes and metabolic processes. Based on the proteomic analysis, several genes involved in secondary metabolism, reactive oxygen burst and phytohormone signaling pathways were identified for further physiological and molecular analysis. The roles of the corresponding genes were further characterized by employing virus-induced gene silencing (VIGS). Based on the results, we suggest that the production of gossypol is sufficient to affect the cotton resistance to V. dahliae. Silencing of GbCAD1, a key enzyme involving in gossypol biosynthesis, compromised cotton resistance to V. dahliae. Reactive oxygen species and salicylic acid signaling may be also implicated as regulators in cotton responsive to V. dahliae according to the analysis of GbSSI2, an important regulator in the crosstalk between salicylic acid and jasmonic acid signal pathways. Moreover, brassinosteroids and jasmonic acid signaling may play essential roles in the cotton disease resistance to V. dahliae. The brassinosteroids signaling was activated in cotton on inoculation with V. dahliae and the disease resistance of cotton was enhanced after exogenous application of brassinolide. Meanwhile, jasmonic acid signaling was also activated in cotton after inoculation with V. dahliae and brassinolide application. These data provide highlights in the molecular basis of cotton resistance to V. dahliae.
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Claims 11
Claim · model-written summary
Proteomics identified 188 differentially expressed proteins.
- Taxon
- cotton
Gossypium hirsutume0t0t1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“188 differentially expressed proteins were identified”
t0“Cotton”
t1“Cotton”
Claim · model-written summary
Proteins were classified into 17 biological processes.
- Taxon
- cottonGossypium hirsutume0t0t1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“classified into 17 biological processes”
t0“Cotton”
t1“Cotton”
Claim · model-written summary
Most proteins related to stimulus, cellular and metabolic processes.
- Taxon
- cottonGossypium hirsutume0t0t1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Most of these proteins were implicated in stimulus response, cellular processes and metabolic processes”
t0“Cotton”
t1“Cotton”
Claim · model-written summary
Gossypol production was suggested to affect cotton resistance.
- Taxon
- cottonGossypium hirsutume0
- Interactor
- V. dahliae
Verticillium dahliaee0pathogen - Compound
- gossypole0
- Outcome
- cotton resistance to V. dahliaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“production of gossypol is sufficient to affect the cotton resistance to V. dahliae”
Claim · model-written summary
GbCAD1 silencing compromised cotton resistance to V. dahliae.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Silencing of GbCAD1e0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- GbCAD1e0
- Outcome
- cotton resistance to V. dahliaee0
- Direction
- compromisede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of GbCAD1, a key enzyme involving in gossypol biosynthesis, compromised cotton resistance to V. dahliae”
Claim · model-written summary
GbSSI2 analysis implicated ROS and SA signaling in cotton response.
- Taxon
- cottonGossypium hirsutume0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Gene
- GbSSI2e0
- Compound
- salicylic acide0
- Outcome
- cotton responsive to V. dahliaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Reactive oxygen species and salicylic acid signaling may be also implicated as regulators in cotton responsive to V. dahliae according to the analysis of GbSSI2”
Claim · model-written summary
Brassinosteroid and jasmonic signaling may contribute to resistance.
- Taxon
- cottonGossypium hirsutume0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Compound
- brassinosteroids and jasmonic acide0
- Outcome
- cotton disease resistance to V. dahliaee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“brassinosteroids and jasmonic acid signaling may play essential roles in the cotton disease resistance to V. dahliae”
Claim · model-written summary
V. dahliae inoculation activated brassinosteroid signaling.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- inoculation with V. dahliaee0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Outcome
- brassinosteroids signalinge0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“brassinosteroids signaling was activated in cotton on inoculation with V. dahliae”
Claim · model-written summary
Brassinolide application enhanced cotton disease resistance.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- exogenous application of brassinolidee0
- Outcome
- disease resistance of cottone0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“disease resistance of cotton was enhanced after exogenous application of brassinolide”
Claim · model-written summary
V. dahliae inoculation activated jasmonic acid signaling.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- inoculation with V. dahliaee0
- Interactor
- V. dahliaeVerticillium dahliaee0pathogen
- Outcome
- jasmonic acid signalinge0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“jasmonic acid signaling was also activated in cotton after inoculation with V. dahliae”
Claim · model-written summary
Brassinolide application activated jasmonic acid signaling.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- brassinolide applicatione0
- Outcome
- jasmonic acid signalinge0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“jasmonic acid signaling was also activated in cotton after inoculation with V. dahliae and brassinolide application”
The same questions in other papers
brassinolide · disease · Gossypium hirsutum (cotton)Results agree
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58 papers in the same direction as this paper · 34 in the opposite direction
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