Abstract
Cotton fiber elongation is a complex developmental process regulated by hormonal and metabolic signals. Auxin (indole-3-acetic acid, IAA) and brassinosteroid (BR) play crucial roles in cotton fiber development, but the mechanism by which they coordinate to regulate fiber elongation remains unclear. In this study, we determined that IAA levels gradually increase during fiber development and are higher in long-fiber varieties. In vitro ovule culture experiments revealed that IAA promotes fiber elongation in a dose-dependent manner. Transcriptome analysis showed that IAA activates BR biosynthesis and the BR signaling pathway, implying crosstalk between the two hormones. Interestingly, IAA could partially rescue fiber elongation caused by BR deficiency due to brassinazole treatment and in pag1 mutants, and BR supplementation partially alleviated fiber inhibition resulting from impaired IAA transport or IAA deficiency. However, neither hormone fully compensated for the absence of the other, indicating that both serve non-redundant roles in fiber elongation. Additionally, inhibiting glucose signaling by suppressing hexokinase activity through N-acetylglucosamine impaired fiber growth, but this could be rescued by exogenous application of either IAA or BR, suggesting that glucose acts upstream of IAA and BR, which cooperatively regulate the elongation of cotton fibers.
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Claims 14
Claim · model-written summary
IAA levels increased during fiber development.
- Taxon
- cotton
Gossypium hirsutumt0 - Organ / stage
- fiber developmente0
- Outcome
- IAA levelse0
- Direction
- increasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA levels gradually increase during fiber development”
t0“cotton”
Claim · model-written summary
IAA levels were higher in long-fiber varieties.
- Taxon
- cottonGossypium hirsutumt0
- Material
- long-fiber varietiese0
- Outcome
- IAA levelse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA levels gradually increase during fiber development and are higher in long-fiber varieties.”
t0“cotton”
Claim · model-written summary
IAA promoted fiber elongation in a dose-dependent manner in ovule culture.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- IAAe0
- Outcome
- fiber elongatione0
- Direction
- promotese0
- Qualifier
- dose-dependent mannere0
- Setting
- In vitro ovule culturee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In vitro ovule culture experiments revealed that IAA promotes fiber elongation in a dose-dependent manner.”
t0“cotton”
Claim · model-written summary
IAA activated BR biosynthesis.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- IAAe0
- Outcome
- BR biosynthesise0
- Direction
- activatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis showed that IAA activates BR biosynthesis and the BR signaling pathway”
t0“cotton”
Claim · model-written summary
IAA activated the BR signaling pathway.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- IAAe0
- Outcome
- BR signaling pathwaye0
- Direction
- activatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis showed that IAA activates BR biosynthesis and the BR signaling pathway”
t0“cotton”
Claim · model-written summary
IAA partially rescued fiber elongation under brassinazole-induced BR deficiency.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- IAAe0
- Outcome
- fiber elongatione0
- Direction
- rescuee0
- Qualifier
- partiallye0
- Comparator
- BR deficiency due to brassinazole treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA could partially rescue fiber elongation caused by BR deficiency due to brassinazole treatment”
t0“cotton”
Claim · model-written summary
IAA partially rescued fiber elongation in pag1 mutants.
- Taxon
- cottonGossypium hirsutumt0
- Material
- pag1 mutantse0
- Treatment
- IAAe0
- Outcome
- fiber elongatione0
- Direction
- rescuee0
- Qualifier
- partiallye0
- Comparator
- pag1 mutantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“IAA could partially rescue fiber elongation caused by BR deficiency due to brassinazole treatment and in pag1 mutants”
t0“cotton”
Claim · model-written summary
BR supplementation partially alleviated fiber inhibition from impaired IAA transport.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- BR supplementatione0
- Outcome
- fiber inhibitione0
- Direction
- alleviatede0
- Qualifier
- partiallye0
- Comparator
- impaired IAA transporte0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BR supplementation partially alleviated fiber inhibition resulting from impaired IAA transport or IAA deficiency.”
t0“cotton”
Claim · model-written summary
BR supplementation partially alleviated fiber inhibition from IAA deficiency.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- BR supplementatione0
- Outcome
- fiber inhibitione0
- Direction
- alleviatede0
- Qualifier
- partiallye0
- Comparator
- IAA deficiencye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“BR supplementation partially alleviated fiber inhibition resulting from impaired IAA transport or IAA deficiency.”
t0“cotton”
Claim · model-written summary
Neither IAA nor BR fully compensated for absence of the other in fiber elongation.
- Taxon
- cottonGossypium hirsutumt0
- Outcome
- non-redundant roles in fiber elongatione0
- Qualifier
- absence of the othere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“neither hormone fully compensated for the absence of the other, indicating that both serve non-redundant roles in fiber elongation.”
t0“cotton”
Claim · model-written summary
Hexokinase suppression by N-acetylglucosamine impaired fiber growth.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- N-acetylglucosaminee0
- Gene
- hexokinasee0
- Outcome
- fiber growthe0
- Direction
- impairede0
- Qualifier
- inhibiting glucose signalinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“inhibiting glucose signaling by suppressing hexokinase activity through N-acetylglucosamine impaired fiber growth”
t0“cotton”
Claim · model-written summary
Exogenous IAA rescued fiber growth impaired by glucose signaling inhibition.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- exogenous application of either IAA or BRe0
- Outcome
- fiber growthe1
- Direction
- rescuede0
- Comparator
- inhibiting glucose signalinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this could be rescued by exogenous application of either IAA or BR”
e1“inhibiting glucose signaling by suppressing hexokinase activity through N-acetylglucosamine impaired fiber growth”
t0“cotton”
Claim · model-written summary
Exogenous BR rescued fiber growth impaired by glucose signaling inhibition.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- BRe0
- Outcome
- fiber growthe1
- Direction
- rescuede0
- Comparator
- inhibiting glucose signalinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this could be rescued by exogenous application of either IAA or BR”
e1“inhibiting glucose signaling by suppressing hexokinase activity through N-acetylglucosamine impaired fiber growth”
t0“cotton”
Claim · model-written summary
Glucose was inferred to act upstream of IAA and BR in regulating cotton fiber elongation.
- Taxon
- cottonGossypium hirsutume0
- Outcome
- elongation of cotton fiberse0
- Qualifier
- glucose acts upstream of IAA and BRe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suggesting that glucose acts upstream of IAA and BR, which cooperatively regulate the elongation of cotton fibers.”
The same questions in other papers
indole-3-acetic acid · quality: grain · Gossypium hirsutum (cotton)Results differ
8 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- positive
- no effect
7 papers more in this group
See all 11 papers →N-acetylglucosamine · quality: grain · Gossypium hirsutum (cotton)Results agree
1 paper in the same direction as this paper
- negative
brassinolide · disease · Gossypium hirsutum (cotton)Results agree
3 papers in the same direction as this paper
- positive
- positive
- positive
brassinolide · quality: grain · Gossypium hirsutum (cotton)Results agree
6 papers in the same direction as this paper
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.