Abstract
In plants, glucose (Glc) plays important roles, as a nutrient and signal molecule, in the regulation of growth and development. However, the function of Glc in fiber development of upland cotton (Gossypium hirsutum) is unclear. Here, using gas chromatography-mass spectrometry (GC-MS), we found that the Glc content in fibers was higher than that in ovules during the fiber elongation stage. In vitro ovule culture revealed that lower Glc concentrations promoted cotton fiber elongation, while higher concentrations had inhibitory effects. The hexokinase inhibitor N-acetylglucosamine (NAG) inhibited cotton fiber elongation in the cultured ovules, indicating that Glc-mediated fiber elongation depends on the Glc signal transduced by hexokinase. RNA sequencing (RNA-seq) analysis and hormone content detection showed that 150mM Glc significantly activated brassinosteroid (BR) biosynthesis, and the expression of signaling-related genes was also increased, which promoted fiber elongation. In vitro ovule culture clarified that BR induced cotton fiber elongation in a dose-dependent manner. In hormone recovery experiments, only BR compensated for the inhibitory effects of NAG on fiber elongation in a Glc-containing medium. However, the ovules cultured with the BR biosynthetic inhibitor brassinazole and from the BR-deficient cotton mutant pag1 had greatly reduced fiber elongation at all the Glc concentrations tested. This demonstrates that Glc does not compensate for the inhibition of fiber elongation caused by BR biosynthetic defects, suggesting that the BR signaling pathway works downstream of Glc during cotton fiber elongation. Altogether, our study showed that Glc plays an important role in cotton fibre elongation, and crosstalk occurs between Glc and BR signaling during modulation of fiber elongation.
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Claims 13
Claim · model-written summary
Glc content in cotton fibers was higher than in ovules during the fiber elongation stage.
- Taxon
- cotton
Gossypium hirsutumt0 - Organ / stage
- fiberse0
- Outcome
- Glc contente0
- Direction
- highere0
- Comparator
- ovulese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“the Glc content in fibers was higher than that in ovules during the fiber elongation stage”
Claim · model-written summary
Lower Glc concentrations promoted cotton fiber elongation in cultured ovules.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- lower Glc concentrationse0
- Outcome
- cotton fiber elongatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“lower Glc concentrations promoted cotton fiber elongation, while higher concentrations had inhibitory effects”
Claim · model-written summary
Higher Glc concentrations had inhibitory effects on cotton fiber elongation in cultured ovules.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- higher concentrationse0
- Outcome
- fiber elongatione0
- Direction
- inhibitory effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“lower Glc concentrations promoted cotton fiber elongation, while higher concentrations had inhibitory effects”
Claim · model-written summary
The hexokinase inhibitor N-acetylglucosamine (NAG) inhibited cotton fiber elongation in cultured ovules.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- hexokinase inhibitor N-acetylglucosamine (NAG)e0
- Outcome
- cotton fiber elongatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“The hexokinase inhibitor N-acetylglucosamine (NAG) inhibited cotton fiber elongation in the cultured ovules”
Claim · model-written summary
Glc-mediated fiber elongation depends on the Glc signal transduced by hexokinase.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- hexokinasee0
- Outcome
- Glc-mediated fiber elongatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“indicating that Glc-mediated fiber elongation depends on the Glc signal transduced by hexokinase”
Claim · model-written summary
150 mM Glc significantly activated brassinosteroid biosynthesis in cotton ovules.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- 150mM Glce0
- Outcome
- brassinosteroid (BR) biosynthesise0
- Direction
- activatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“150mM Glc significantly activated brassinosteroid (BR) biosynthesis”
Claim · model-written summary
150 mM Glc also increased the expression of brassinosteroid signaling-related genes.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- 150mM Glce0
- Gene
- signaling-related genese0
- Outcome
- expression of signaling-related genese0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“150mM Glc significantly activated brassinosteroid (BR) biosynthesis, and the expression of signaling-related genes was also increased”
Claim · model-written summary
BR induced cotton fiber elongation in a dose-dependent manner in cultured ovules.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- BRe0
- Outcome
- cotton fiber elongatione0
- Direction
- inducede0
- Qualifier
- dose-dependent mannere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“BR induced cotton fiber elongation in a dose-dependent manner”
Claim · model-written summary
Among hormones tested, only BR compensated for the inhibitory effects of NAG on fiber elongation in a Glc-containing medium.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- BRe0
- Outcome
- inhibitory effects of NAG on fiber elongatione0
- Direction
- compensatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“only BR compensated for the inhibitory effects of NAG on fiber elongation in a Glc-containing medium”
Claim · model-written summary
Ovules cultured with the BR biosynthetic inhibitor brassinazole had greatly reduced fiber elongation at all Glc concentrations tested.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- BR biosynthetic inhibitor brassinazolee0
- Outcome
- fiber elongatione0
- Direction
- greatly reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“the ovules cultured with the BR biosynthetic inhibitor brassinazole and from the BR-deficient cotton mutant pag1 had greatly reduced fiber elongation at all the Glc concentrations tested”
Claim · model-written summary
Ovules from the BR-deficient cotton mutant pag1 had greatly reduced fiber elongation at all Glc concentrations tested.
- Taxon
- cottonGossypium hirsutume0
- Material
- BR-deficient cotton mutant pag1e0
- Outcome
- fiber elongatione0
- Direction
- greatly reducede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“the ovules cultured with the BR biosynthetic inhibitor brassinazole and from the BR-deficient cotton mutant pag1 had greatly reduced fiber elongation at all the Glc concentrations tested”
Claim · model-written summary
Glc did not compensate for the inhibition of fiber elongation caused by BR biosynthetic defects.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Glce0
- Outcome
- inhibition of fiber elongation caused by BR biosynthetic defectse0
- Direction
- does not compensatee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“Glc does not compensate for the inhibition of fiber elongation caused by BR biosynthetic defects”
Claim · model-written summary
The BR signaling pathway works downstream of Glc during cotton fiber elongation.
- Taxon
- cottonGossypium hirsutume0
- Outcome
- cotton fiber elongatione0
- Qualifier
- BR signaling pathway works downstream of Glce0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“cotton”
e0“suggesting that the BR signaling pathway works downstream of Glc during cotton fiber elongation”
The same questions in other papers
N-acetylglucosamine · quality: grain · Gossypium hirsutum (cotton)Results agree
1 paper in the same direction as this paper
- negative
brassinazole · quality: grain · Gossypium hirsutum (cotton)Results agree
2 papers in the same direction as this paper
- negative
- negative
brassinolide · quality: grain · Gossypium hirsutum (cotton)Results agree
6 papers in the same direction as this paper
- positive
- positive
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.