Abstract
Fibers are the main economic product of cotton (Gossypium hirsutum). As an ideal model for studying plant cell development, many factors that influence cotton fiber have been revealed. Previous studies have demonstrated the important role of sphingolipids in fiber development through chemical and genetic methods, but the regulatory module by which sphingolipids regulate fiber development remains unclear. Here, we used the sphingolipid biosynthesis inhibitor fumonisin B1 (FB1) and found that it effectively suppressed the expression of an orthologue of the trihelix transcription factor SIP1 clade-like (GhSIL) in upland cotton. Lipid-protein binding assays also revealed that ceramide (t18:0/24:0) and 24-epibrassinolide (BL) bind to GhSIL protein in vitro. GhSIL was preferentially expressed in elongating fibers. The transcriptome and reverse transcription quantitative PCR analysis revealed that the expression of brassinosteroid synthesis genes was significantly decreased in GhSIL downregulated cotton fibers during the fiber elongation stage. Yeast 1-hybrid assay, dual-luciferase (LUC) assay, and chromatin immunoprecipitation (ChIP) assays showed that GhSIL can directly bind to the GT1-box element in the brassinosteroid-6-oxidase 2 (GhBR6OX2) promoter region to enhance its expression. Overexpression of GhSIL and GhBR6OX2 promoted fiber elongation, consistent with the results of exogenous application of BL in cotton ovule culture in vitro. By contrast, suppressing GhSIL and GhBR6OX2 expression inhibited fiber elongation, which was similar to the exogenous application of brassinazole (BRZ). These results illuminated that sphingolipids regulate fiber cell elongation through the GhSIL-GhBR6OX2-brassinolide module, which reveals a node in the molecular mechanism by which sphingolipids regulate plant growth and development.
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Claims 12
Claim · model-written summary
Fumonisin B1 suppressed GhSIL expression in upland cotton.
- Taxon
- upland cottone0
- Treatment
- fumonisin B1 (FB1)e0
- Gene
- GhSILe0
- Outcome
- expression of an orthologue of the trihelix transcription factor SIP1 clade-like (GhSIL)e0
- Direction
- suppressede0
Not reportedComparator
Verbatim
e0“the sphingolipid biosynthesis inhibitor fumonisin B1 (FB1) and found that it effectively suppressed the expression of an orthologue of the trihelix transcription factor SIP1 clade-like (GhSIL) in upland cotton”
Claim · model-written summary
Ceramide bound GhSIL protein in vitro.
- Taxon
- cotton
Gossypium hirsutumt0 - Gene
- GhSILe0
- Compound
- ceramide (t18:0/24:0)e0
- Outcome
- bind to GhSIL proteine0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ceramide (t18:0/24:0) and 24-epibrassinolide (BL) bind to GhSIL protein in vitro”
t0“cotton”
Claim · model-written summary
24-epibrassinolide bound GhSIL protein in vitro.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhSILe0
- Compound
- 24-epibrassinolide (BL)e0
- Outcome
- bind to GhSIL proteine0
- Setting
- in vitroe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ceramide (t18:0/24:0) and 24-epibrassinolide (BL) bind to GhSIL protein in vitro”
t0“cotton”
Claim · model-written summary
GhSIL was preferentially expressed in elongating fibers.
- Taxon
- cottonGossypium hirsutumt0
- Organ / stage
- elongating fiberse0
- Gene
- GhSILe0
- Outcome
- preferentially expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhSIL was preferentially expressed in elongating fibers”
t0“cotton”
Claim · model-written summary
GhSIL downregulation significantly decreased brassinosteroid synthesis gene expression during fiber elongation.
- Taxon
- cottonGossypium hirsutume0
- Organ / stage
- fiber elongation stagee0
- Treatment
- GhSIL downregulatede0
- Gene
- brassinosteroid synthesis genese0
- Outcome
- expression of brassinosteroid synthesis genese0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression of brassinosteroid synthesis genes was significantly decreased in GhSIL downregulated cotton fibers during the fiber elongation stage”
Claim · model-written summary
GhSIL bound the GT1-box of the GhBR6OX2 promoter and enhanced its expression.
- Taxon
- cottonGossypium hirsutumt0
- Gene
- GhSILe0
- Outcome
- enhance its expressione0
- Qualifier
- bind to the GT1-box element in the brassinosteroid-6-oxidase 2 (GhBR6OX2) promoter regione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhSIL can directly bind to the GT1-box element in the brassinosteroid-6-oxidase 2 (GhBR6OX2) promoter region to enhance its expression”
t0“cotton”
Claim · model-written summary
GhSIL overexpression promoted cotton fiber elongation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- Overexpression of GhSILe0
- Outcome
- fiber elongatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of GhSIL and GhBR6OX2 promoted fiber elongation”
t0“cotton”
Claim · model-written summary
GhBR6OX2 overexpression promoted cotton fiber elongation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- GhBR6OX2e0
- Outcome
- fiber elongatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of GhSIL and GhBR6OX2 promoted fiber elongation”
t0“cotton”
Claim · model-written summary
Exogenous BL promoted cotton fiber elongation in ovule culture.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- exogenous application of BLe0
- Outcome
- fiber elongatione0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of GhSIL and GhBR6OX2 promoted fiber elongation, consistent with the results of exogenous application of BL in cotton ovule culture in vitro”
Claim · model-written summary
Suppressing GhSIL expression inhibited cotton fiber elongation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- suppressing GhSILe0
- Outcome
- fiber elongatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppressing GhSIL and GhBR6OX2 expression inhibited fiber elongation”
t0“cotton”
Claim · model-written summary
Suppressing GhBR6OX2 expression inhibited cotton fiber elongation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- GhBR6OX2 expressione0
- Outcome
- fiber elongatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppressing GhSIL and GhBR6OX2 expression inhibited fiber elongation”
t0“cotton”
Claim · model-written summary
Exogenous brassinazole inhibited cotton fiber elongation.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- exogenous application of brassinazole (BRZ)e0
- Outcome
- fiber elongatione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“suppressing GhSIL and GhBR6OX2 expression inhibited fiber elongation, which was similar to the exogenous application of brassinazole (BRZ)”
t0“cotton”
The same questions in other papers
brassinazole · quality: grain · Gossypium hirsutum (cotton)Results agree
2 papers in the same direction as this paper
- negative
- negative
class · quality: grain · Gossypium hirsutum (cotton)Positive and negative results
4 papers in the same direction as this paper · 6 in the opposite direction
- positive
- positive
- positive
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.