Abstract
C5 volatile compounds, derived from fatty acids, are among the most important contributors to consumer liking of fresh tomatoes. Despite their important roles in flavour, the genes responsible for C5 volatile synthesis have yet to be identified. This work shows that their synthesis is catalysed in part by a 13-lipoxygenase (LOX), TomloxC, the same enzyme responsible for synthesis of C6 volatiles. C5 synthesis is independent of hydroperoxide lyase (HPL); moreover, HPL knockdown significantly increased C5 volatile synthesis. This LOX-dependent, HPL-independent pathway functions in both fruits and leaves. Synthesis of C5 volatiles increases in leaves following mechanical wounding but does not increase in response to infection with Xanthomonas campestris pv. vesicatoria. Large reductions in C5 and C6 volatiles in antisense TomloxC knockdown plants were observed but those reductions did not alter the development of disease symptoms, indicating that these volatiles do not have an important defensive function against this bacterial pathogen.
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Claims 10
Claim · model-written summary
TomloxC catalyses part of C5 volatile synthesis.
- Taxon
- tomato
Solanum lycopersicumt0 - Gene
- TomloxCe0
- Outcome
- synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“their synthesis is catalysed in part by a 13-lipoxygenase (LOX), TomloxC”
t0“tomato”
Claim · model-written summary
TomloxC also catalyses C6 volatile synthesis.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- TomloxCe1
- Outcome
- synthesis of C6 volatilese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the same enzyme responsible for synthesis of C6 volatiles”
e1“a 13-lipoxygenase (LOX), TomloxC, the same enzyme”
t0“tomato”
Claim · model-written summary
C5 synthesis is independent of hydroperoxide lyase.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- hydroperoxide lyase (HPL)e0
- Outcome
- C5 synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“C5 synthesis is independent of hydroperoxide lyase (HPL)”
t0“tomato”
Claim · model-written summary
HPL knockdown significantly increased C5 volatile synthesis.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- HPL knockdowne0
- Gene
- HPLe0
- Outcome
- C5 volatile synthesise0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“HPL knockdown significantly increased C5 volatile synthesis”
t0“tomato”
Claim · model-written summary
The LOX-dependent, HPL-independent C5 pathway functions in fruits and leaves.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- fruits and leavese0
- Outcome
- pathway functions in both fruits and leavese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This LOX-dependent, HPL-independent pathway functions in both fruits and leaves”
t0“tomato”
Claim · model-written summary
Mechanical wounding increased leaf C5 volatile synthesis.
- Taxon
- tomatoSolanum lycopersicumt0
- Organ / stage
- leavese0
- Treatment
- mechanical woundinge0
- Outcome
- Synthesis of C5 volatilese0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Synthesis of C5 volatiles increases in leaves following mechanical wounding”
t0“tomato”
Claim · model-written summary
Xanthomonas infection did not increase leaf C5 volatile synthesis.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- infection with Xanthomonas campestris pv. vesicatoriae0
- Interactor
- Xanthomonas campestris pv. vesicatoria
_(25868012152).jpg?width=60)
Xanthomonas campestrise0pathogen - Outcome
- Synthesis of C5 volatilese1
- Direction
- does not increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_(25868012152).jpg?width=500)
Verbatim
e0“does not increase in response to infection with Xanthomonas campestris pv. vesicatoria”
e1“Synthesis of C5 volatiles increases in leaves”
t0“tomato”
Claim · model-written summary
Antisense TomloxC knockdown greatly reduced C5 volatiles.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- antisense TomloxC knockdowne0
- Gene
- TomloxCe0
- Outcome
- C5e0
- Direction
- reductionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Large reductions in C5 and C6 volatiles in antisense TomloxC knockdown plants were observed”
t0“tomato”
Claim · model-written summary
Antisense TomloxC knockdown greatly reduced C6 volatiles.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- antisense TomloxC knockdowne0
- Gene
- TomloxCe0
- Outcome
- C6 volatilese0
- Direction
- reductionse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Large reductions in C5 and C6 volatiles in antisense TomloxC knockdown plants were observed”
t0“tomato”
Claim · model-written summary
Reduced C5 and C6 volatiles did not alter bacterial disease symptom development.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- those reductionse0
- Interactor
- Xanthomonas campestris pv. vesicatoriaXanthomonas campestrise1pathogen
- Outcome
- development of disease symptomse0
- Direction
- did not altere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those reductions did not alter the development of disease symptoms”
e1“Xanthomonas campestris pv. vesicatoria”
t0“tomato”
The same questions in other papers
infection · quality: phytochemicals · Solanum lycopersicum (tomato)Positive and negative results
9 papers in the same direction as this paper · 7 in the opposite direction
- positive
- positive
- positive
13 papers more in this group
See all 17 papers →mechanical · quality: phytochemicals · Solanum lycopersicum (tomato)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.